American Public Gardens Association

The COVID-19 pandemic demonstrated both the transformative capacity of vaccine innovation and the persistent inequities that accompany emergency access, underscoring the critical need for stronger collaboration between global health governance and the vaccine industry. Influenza pandemics remain inevitable threats. The continued emergence of avian influenza strains such as H5N1 reinforces the necessity of robust preparedness. This perspective examines the underutilization of private sector vaccine manufacturers in current pandemic influenza frameworks and identifies three central areas where industry participation is indispensable: predictable vaccine demand through robust seasonal influenza programs, economic incentives that de-risk investments in research and development, and diversification of vaccine platforms to expand response capacity. In addition, regionalizing manufacturing, advancing collaborative regulatory models, and negotiating export waivers are presented as potential mechanisms to strengthen equity and supply security. The review highlights demand-based tiered pricing and Advance Purchase Agreements as practical tools to align commercial incentives with public health priorities. Furthermore, it makes the case for embedding private sector representation and knowledge into top-level decision-making and preparedness planning, ensuring investment in innovation is aligned with global health objectives. Ultimately, true pandemic influenza readiness depends on building a sustained seasonal influenza market, embedding private sector engagement into governance structures, and fostering mutual trust to ensure timely access and equitable protection for populations worldwide.

The ecological and developmental history of the Chicago, Illinois, region has affected the current distribution of forests therein. These same factors, along with systemic and long‐lasting racial segregation, have shaped the distribution of the urban populations that benefit from the ecosystem services provided by urban forests. This study demonstrates that forest patch history is related to forest attributes like tree species composition, tree density, canopy height, and structural heterogeneity—all of which are important predictors of a forest's ability to provide ecosystem services. However, this effect of forest history was only seen in forest cores, as forest edges were similar regardless of patch history. We also found that forests in minoritized communities tended to be less able to support high levels of ecosystem services. This research indicates that, when improving green equity, it is important to consider the variable capacity of forests to provide ecosystem services.

A gardening methodology using double-cropped cool-season vegetables and warm-season turfgrass, thereby capitalizing on the ideal growing season for each, was developed in field trials and tested in volunteers’ landscapes. Broccoli (Brassica oleracea), lettuce (Lactuca sativa), and Swiss chard (Beta vulgaris subsp. Cicla) were planted into an established hybrid bermudagrass lawn (Cynodon dactylon (L.) Pers. × C. transvaalensis Burtt-Davy ‘Tifsport’) in September. The vegetables were planted into tilled strips, 5 cm × 10 cm holes and 10 cm × 10 cm holes in the turf. All treatments produced harvestable yield, though the yield of vegetables planted in the tilled treatments and larger holes was greater than in smaller holes. Efforts to reduce turfgrass competition with vegetables by the application of glyphosate or the use of the Veggie Lawn Pod (an easily installed plastic cover on the lawn) did not increase yield. Tilled treatments left depressions that discouraged spring turfgrass recovery. The double-crop was tested by seven volunteers on their lawns. Though lawn-planted vegetables did not produce as much yield as those planted in the volunteers’ gardens, the volunteers were enthusiastic about this methodology. The volunteers reported that lawn vegetables were more difficult to plant but not more difficult to maintain, and they were easier to harvest than vegetables in their gardens. All volunteers reported satisfactory recovery of their lawns in the spring.

Clostridioides difficile is an important enteric pathogen that causes significant morbidity and mortality in humans. With community‐acquired infections on the rise, it is important to identify reservoirs of the pathogen. Companion animals can be asymptomatic carriers of C. difficile and may therefore represent a reservoir, but epidemiological studies of C. difficile within the pet‐owner unit are needed, along with validated methods to detect C. difficile in both people and animals. The goal of this study was to assess the performance of commercial qPCR assays and a multiplex PCR for C. difficile compared to toxigenic culture. These assays were tested on up to 103 fecal samples from puppies, a population in which the prevalence of C. difficile is the highest. The sensitivities, specificities, positive predictive values and negative predictive values were respectively 84.2%, 87.7%, 61.5%, and 95.9% for the Cepheid GeneXpert; 66.7%, 66.7%, 29.6%, and 90.9% for the DiaSorin Simplexa; and 94.4%, 85.0%, 65.4%, and 98.1%, for the multiplex qPCR. The agreement was highest between the GeneXpert and the multiplex PCR (90.1% agreement, with a kappa statistic of 0.77). For diagnostic purposes, the positive predictive values of the assays were low. However, the high sensitivities of the assays could render them useful for epidemiologic purposes.

Shortnose Sturgeon = SNS (Acipenser brevirostrum) is a small diadromous species with most populations living in large Atlantic coast rivers and estuaries of North America from New Brunswick, Canada, to GA, USA. There are no naturally land-locked populations, so all populations require access to fresh water and salt water to complete a natural life cycle. The species is amphidromous with use of fresh water and salt water (the estuary) varied across the species range, a pattern that may reflect whether freshwater or saltwater habitats provide optimal foraging and growth conditions. Migration is a dominant behaviour during life history, beginning when fish are hatchling free embryos (southern SNS) or larvae (northeastern and far northern SNS). Migration continues by juveniles and non-spawning adult life stages on an individual time schedule with fish moving between natal river and estuary to forage or seek refuge, and by spawning adults migrating to and from riverine spawning grounds. Coastal movements by adults throughout the range (but particularly in the Gulf of Maine = GOM and among southern rivers) suggest widespread foraging, refuge use, and widespread colonization of new rivers. Colonization may also be occurring in the Potomac River, MD–VA–DC (mid-Atlantic region). Genetic studies (mtDNA and nDNA) identified distinct individual river populations of SNS, and recent range-wide nDNA studies identified five distinct evolutionary lineages of SNS in the USA: a northern metapopulation in GOM rivers; the Connecticut River; the Hudson River; a Delaware River–Chesapeake Bay metapopulation; and a large southern metapopulation (SC rivers to Altamaha River, GA). The Saint John River, NB, Canada, in the Bay of Fundy (north of the GOM), is the sixth distinct genetic lineage within SNS. Life history information from telemetry tracking supports the genetic information documenting extensive movement of adults among rivers within the three metapopulations. However, individual river populations with spawning adults are still the best basal unit for management and recovery planning. The focus on individual river populations should be complemented with attention to migratory processes and corridors that foster metapopulation level risks and benefits. The species may be extirpated at the center of the range, i.e., the mid-Atlantic region (Chesapeake Bay, MD–VA, and probably, NC), but large rivers in VA, including the James and Potomac rivers, need study. The largest SNS populations in GOM and northeastern rivers, like the Kennebec, Hudson, and Delaware rivers, typically have tens of thousands of adults. This contrasts with southern rivers, where rivers typically have much fewer (<2500) adults, except for the Altamaha River (>6000 adults). River damming in the 19th and 20th Centuries extirpated some populations, and also, created two dysfunctional segmented populations: the Connecticut River SNS in CT–MA and the Santee-Cooper rivers–Lake Marion SNS in SC. The major anthropogenic impact on SNS in marine waters is fisheries bycatch. The major impacts that determine annual recruitment success occur in freshwater firstly, where adult spawning migrations and spawning are blocked or spawning success is affected by river regulation and secondly, where poor survival of early life stages is caused by river dredging, pollution, and unregulated impingement-entrainment in water withdrawal facilities. Climate warming has the potential to reduce abundance or eliminate SNS in many rivers, particularly in the South. In 1998, the National Marine Fisheries Service (NMFS) recommended management of 19 rivers as distinct population segments (DPSs) based on strong fidelity to natal rivers. A Biological Assessment completed in 2010 reaffirmed this approach. NMFS has not formally listed DPSs under the ESA and the species remains listed as endangered range-wide in the USA.

Slow growth rate of plantlets, few micro-shoots per explant, and slow root growth rate are restrictions of in vitro propagation of poinsettia ( Euphorbia pulcherrima Willd. ex Koltz). The purpose of this research was to develop an efficient in vitro proliferation technique for poinsettia ‘Prestige™ Red’. Explants (apical buds and axillary buds) placed on Murashige and Skoog (MS) basal medium containing only 6-benzylaminopurine (BA) and combinations of BA and indole-3-acetic acid (IAA) mostly produced red callus, which is productive and some white and gray–green calluses at the base of plantlets after 1 month, whereas explants in a medium without plant growth regulators (PGRs) produced no callus. Addition of IAA into the rooting medium increased rooting efficiency; plantlets grown in half-strength MS salts and vitamins with 28.5 μM IAA initiated rooting 11 days earlier than the plantlets grown with no PGRs. Optimization of PGR concentrations during poinsettia micropropagation helped resolve previous restrictions of in vitro poinsettia proliferation. Chemical names used: 6-benzylaminopurine (BA); indole-3-acetic acid (IAA)

Foals may present to a referral hospital with the primary diagnosis of uroperitoneum (UP), or they may develop UP while hospitalized for other reasons. Historical, physical, laboratory, and diagnostic variables of foals presenting with UP were compared to those developing UP while hospitalized. Emphasis was placed on the presence of electrolyte abnormalities, evidence of sepsis or infection, and development of anesthetic complications during surgical correction of the defect. Foals developing UP while in the hospital frequently had a history of dystocia and presented at a very young age (< 48 hours) with primary clinical signs compatible with intrauterine compromise or presumed hypoxic or ischemic insult with or without sepsis. Foals referred with suspected UP often had additional problems unrelated to the urinary system. These foals had hyponatremia and hyperkalemia on presentation, whereas foals receiving intravenous fluid therapy consisting of a balanced electrolyte solution did not develop the classical pattern of electrolyte abnormalities, yet a similar increase in serum creatinine and, frequently, decreasing urine production were noted. Infection was present in 63% of the foals, and 78% of foals revealed signs suggestive of sepsis or infection. Intrauterine compromise, presumed hypoxia or ischemia, and sepsis may predispose foals to development of UP. Anesthetic complications occurred in 16% of the foals undergoing surgical correction of the defect, although hyperkalemia was only present in half of the foals with anesthetic complications.

Micropropagation of three Echinacea species, E. angustifolia DC., E. pallida Nutt., and E. purpurea Moench., was investigated as a potential means of germplasm preservation of species faced with overcollection in the wild and rapid clonal propagation of elite individuals with unique medicinal or ornamental properties. Very high contamination rates occurred with shoot-tip explants but not with nodal segments. Contamination rates for seed explants were inversely related to the number of seedcoat layers removed, ranging from 100% contamination from intact seeds to near 0% contamination from excised embryos. Dormancy of seed explants was also eliminated when the pericarp and integument were removed. Addition of benzyladenine (BA) to the culture medium induced shoot multiplication and inhibited root formation in all three species. Shoot multiplication rates were low (1-3 shoots per culture) when seed explants were placed on a medium with BA levels low enough to avoid adventitious shoot formation (0.45 μ m ). Shoot count was higher on half-strength Murashige and Skoog (MS) minerals, while leaf size was greater on full-strength MS minerals. Cultures did not perform well in Woody Plant Medium. Reducing subculture frequency from 4 to 2 weeks increased shoot multiplication from 1.4 to 1.8 shoots per subculture and total shoots produced per subculture after 12 weeks from 2.8 to 23.9. Rooting occurred readily on shoots isolated from E. purpurea cultures and was not promoted by addition of IBA to the rooting medium. Rooting was low and nil on shoots from cultures of E. angustifolia and E. pallida , respectively. Methods described in this study allow rapid multiplication of three Echinacea species and subsequent rooting of E. purpurea . Future improvements in root induction treatments will allow these methods to be used effectively for micropropagation and maintenance of disease-free germplasm of Echinacea species. Chemical names used: N -(phenylmethyl)-1 H -purine-6-amine (BA); 1 H -indole-3-butyric acid (IBA).