Beihai People's Hospital

Pyroptosis inhibition via Fe single-atom nanozymes is promising for inflammation therapy, but the common Fe-N4 configuration restricts oxygen intermediate desorption and lacks cooperative sites, thus limiting catalytic performance. To overcome this, we develop a FeMn dual-atom nanozyme supported on oxygen-nitrogen-doped bamboo-like carbon nanotubes (FeMnDA/BCNT). Through the precise alignment of Fe and Mn 3dz2 orbital energy levels by the electron-delocalized BCNT support in the FeMn-N/O active center, thereby lowering the dissociation energy barrier for *O2 or *H2O molecules, promoting O─O bond cleavage to bypass toxic ─OOH species, and thus accelerating the enzyme-like kinetics. Combined with a hierarchical porous bamboo-like structure of the BCNT that enhances high specific surface, atom exposure, and mass transfer, the FeMnDA/BCNT nanozymes exhibit potent superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx)-like activities. Further encapsulation with a macrophage membrane ([MM]FeMnDA/BCNT) confers excellent biocompatibility and active targeting ability toward inflammatory sites. The resulting [MM]FeMnDA/BCNT nanozymes target the inflammatory microenvironment, scavenges ROS, restores mitochondrial function, and suppresses NLRP3 inflammasome activation, thereby inhibiting pyroptosis. In vivo, [MM]FeMnDA/BCNT nanozymes show good biocompatibility and efficacy in treating osteoarthritis, acute liver injury, and acute kidney injury. This work provides a novel strategy for inflammatory disease therapy using a biomimetic dual-atom nanozyme.

The objective was to assess whether amylase (AMY) and other laboratory parameters have a prognostic value for patients with Paraquat (PQ) poisoning. Standard mean difference and 95% confidence intervals were calculated to compare the endpoints. In total, 17 studies comprising 1673 patients were enrolled, 731 patients (43.69%) were defined as survivors, while 942 patients (56.31%) were considered non-survivors. The levels of AMY and plasma PQ were significantly higher in non-survivors when compared to subjects in the survival group. The white blood cell count, concentration of C-reactive protein, creatinine, blood urea nitrogen, alanine aminotransferase and asparagine transaminase were significantly increased in the non-survivor group, while serum potassium, pH and PaCO2 were decreased in the non-survivor group. In addition, PaO2 values were similar between the two groups. Notably, heterogeneity was a key feature across the analyses: most primary and secondary prognostic indicators exhibited high to extreme heterogeneity (I² range: 0.0%-96.7%), with the highest heterogeneity observed for CRP (I²=96.7%) and PaO2 (I²=90.1%). A lower level of AMY and plasma PQ in poisoning patients might have a good prognosis value.

Graphic abstract. The correlation diagrams showing the genetic relation between tuffaceous rocks and Li-rich karst bauxite deposits. • The Upper Permian Linghao Formation tuffaceous rocks and Pingguo Li-rich karst bauxite deposits in western Guangxi, China represent contemporaneous-heterophase deposits formed at ∼259 Ma. • A major volcanic eruption episode supplied the source materials for both the Linghao Formation tuffaceous rocks and Li-rich karst bauxite deposits. • The primary volcanic ash source exhibits definitive arc-related with minor mantle-derived contributions, thereby identifying the Paleo-Tethyan magmatic arc as the dominant provenance. Karst bauxite deposits are an important potential lithium resource, distinct from grantie-pegmatite, brine, and volcanic-sedimentary lithium. Pingguo Li-rich karst bauxite deposits in Youjiang Basin, South China represent major deposits hosted in the Heshan Formation deposited on the Late Permian carbonate platform. Contemporaneous-heterophase tuffaceous rocks of the Upper Permian Linghao Formation distribute widely surrounding the Pingguo Carbonate Platform. The tuffaceous rocks provide a definitive tracer for the provenance of Li-rich karst bauxite deposits. Integrated geochemical, zircon U-Pb chronology, and in situ Hf isotopic analyses reveal that both tuffaceous rocks and Pingguo Li-rich karst bauxite deposits formed synchronously at ∼ 259 Ma from the same source. The correlations of Zr-Hf, Nb-Ta, and Th/Sc-Zr/Sc confirm the genetic relation between tuffaceous rocks and Li-rich karst bauxite. Zircon trace elements fingerprint arc-related signature of the parent source. Critically, the Hf isotope compositions of the tuff (ε Hf (t)≈-4.0, T DM2 ≈1.5 Ga) indicate unequivocally with the Paleo-Tethyan magmatic arc, demonstrating that volcanic ash derived from Paleo Tethys. The provenance of Li-rich karst bauxite deposits in Pingguo Carbonate Platform was volcanic ash from Paleo-Tethyan arc. The volcanic ash covered the carbonate platform during the latest Middle Permian and early Late Permian and resulted in the formation of Li-rich karst bauxite, revealing the Paleo-Tethyan arc as the main source for the significant deposits.

The post-coronavirus disease 2019 (COVID-19) pulmonary sequelae have garnered public concern. We conducted a multicenter cross-sectional study in outpatient and health exam populations from 23 clinical centers (including university-affiliated/provincial general hospitals, municipal general hospitals, county hospitals, and specialized hospitals) in China (2019-2023), to assess temporal trends and potential influencing factors in the detection of CT-diagnosed pulmonary nodules, pleural effusion, pneumonia, and suspected lung tumors, cancer and viral pneumonia, clarifying pandemic impacts on lung health. Dynamic comparisons across key phases including initial outbreak, vaccine rollout, population-wide vaccination, and major adjustment of pandemic control policies, were performed. This study analyzed 1,616,750 clinical samples (1,102,605 outpatient, 514,145 health examination; 885,945 males, 730,805 females). Pulmonary nodule detection rose progressively, with surges in 2020-2021 and 2023, plateauing in 2021-2022. Outpatients and males showed steeper increases. University-affiliated/provincial hospitals had sharpest increases vs. municipal and county tiers. Specialized hospitals matched general hospital rates. AI boosted detection rates. CT-suspected lung tumors/cancer remained low and stable, unrelated to nodule trends. These results underscore 2019-2023 pulmonary nodule detection surges linked to SARS-CoV-2 infections and AI adoption. COVID-19 vaccination did not accelerate detection but may have slowed it short-term. Long-term studies on infection, vaccine impacts and pandemic-detected nodules' outcomes are urgently needed.

This study takes the children's animation IP “Brave Brother Ah Niu” as a case study, where the animation IP refers not merely to a single work, but rather to a core content asset capable of multi-dimensional development and possessing long-term commercial value, such as a well-known character, story, or worldview. This case is used to explore the path for children's animation IP to empower the deep integration of cultural tourism based on Bourdieu's field theory. In response to the current challenges of fragmentation, superficial symbolization, and capital feedback disruption in development, a three-field transformation framework of “cultural production-media practice-industrial consumption” is constructed, and an integration mechanism of “empowerment-translation-feedback” is proposed. This study provides a replicable theoretical model and practical path for the Ah Niu IP and similar regional children's animation IPs to overcome the bottleneck of cultural tourism integration, achieve cultural inheritance, and enhance the industrial value.

ObjectiveThis study aimed to investigate the correlation between anti-phospholipase A2 receptor (PLA2R) levels in serum and urine with clinical parameters, particularly albuminuria, in patients with idiopathic membranous nephropathy (IMN).MethodsWe retrospectively analyzed data from 30 patients with biopsy-proven PLA2R-related IMN diagnosed between 2016 and 2022 at two medical centers. Serum and urine anti-PLA2R antibody levels were measured using enzyme-linked immunosorbent assay (ELISA). We assessed the correlation between antibody levels and clinical parameters, including plasma albumin, 24-hour urine protein quantification, and estimated glomerular filtration rate (eGFR). Patients were staged according to the Ehrenreich-Churg classification.ResultsSerum anti-PLA2R antibody levels showed a significant negative correlation with plasma albumin (r = -0.469, P < 0.05), whereas urine anti-PLA2R antibody levels exhibited a weak, but significant, positive correlation with 24-hour urine protein excretion (r = 0.362, P = 0.049). Patients with higher serum anti-PLA2R antibody titers had significantly lower plasma albumin levels (20.63 ± 4.79 g/L) compared to those with lower titers (27.71 ± 6.78 g/L) (P < 0.05). Conversely, patients with higher urine anti-PLA2R antibody titers had significantly higher 24-hour urine protein quantification (9.22 ± 4.17 g) compared to those with lower titers (5.32 ± 3.09 g) (P < 0.05).ConclusionIn patients with IMN, serum anti-PLA2R antibody concentrations are inversely associated with plasma albumin, while urine anti-PLA2R antibody levels are positively associated with 24-hour urine protein quantification. These findings suggest that combined assessment of serum and urine anti-PLA2R antibody levels may provide valuable insights into disease activity and albuminuria in IMN. Further studies with larger cohorts are needed to validate these findings and explore their potential clinical implications.

This study aims to evaluate the impact of the traditional Chinese medicine formula “Pinggan Jiedu Formula” combined with antiretroviral therapy (ART) on the transcriptional and immune status of hepatitis B virus (HBV) in patients with HIV/HBV co-infection. A total of 62 patients with co-infection of HIV/HBV were included in the study and randomly divided into two groups. In the combined treatment group, 31 patients received PGJDP combined with ART treatment for 24 weeks and then continued to receive ART treatment alone. In the control group, 31 cases received ART treatment throughout the course. The study employed real-time quantitative polymerase chain reaction (RT-qPCR) to detect HBV DNA, HIV RNA, HBV RNA and HIV DNA, and to evaluate the viral replication level and transcriptional activity. Flow cytometry was used to detect the count of CD4 + T cells and the CD4/CD8 ratio in peripheral blood, reflecting the immune status of the patients. The level of HBsAg in serum was detected by enzyme-linked immunosorbent assay (ELISA) to monitor the effect of antiviral treatment. Meanwhile, transient elastography was used to detect liver stiffness (LSM) and calculate the FIB-4 index to evaluate the degree of liver fibrosis. The research results show that compared with the control group, the PGJDP combined treatment group significantly reduced the HBV RNA level, significantly decreased the HBsAg level, significantly increased the CD4 + T cell count, and significantly improved the CD4/CD8 ratio. The PGJDP combined treatment group also showed significant improvement in liver fibrosis indicators. In terms of the incidence of abnormal liver function, the PGJDP combined treatment group was significantly lower than the control group. These results suggest that the combination therapy of PGJDP has potential therapeutic benefits in inhibiting HBV transcription and enhancing the immune response. • To evaluate the effects of “Pinggan Jiedu Prescription” (PGJDP) in combination with antiretroviral therapy (ART) on hepatitis B virus (HBV) transcription and immune status in individuals co-infected with human immunodeficiency virus (HIV) and HBV. • PGJDP combination therapy group showed enhanced hepatic protection and a significantly lower incidence of liver function abnormalities. • Providing a novel therapeutic strategy for HIV-HBV co-infected patients through the integration of PGJDP with ART.

BACKGROUNDChronic hepatitis B (CHB) affects millions worldwide, and many patients develop depression and anxiety. The disease’s long-term nature, potential complications, and social stigma contribute to these mental-health issues. However, previous studies on this link differed in their methods and results, making it hard to draw clear conclusions. This study aimed to analyze factors associated with CHB through meta-analysis of previous studies to help improve patients’ mental health.AIMTo systematically search, screen, and comprehensively analyze existing relevant research through meta-analysis of previous studies to assess the correlation of the previously identified factors found to be associated with comorbid depression and anxiety in patients with CHB, with the goal of improving the patients' mental-health status.METHODSThis study strictly adhered to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses statement. Relevant literature from the inception of the PubMed, EMBASE, Cochrane Library, and Web of Science databases up to January 2025 was comprehensively searched. The search used medical subject headings combined with free-text terms, supplemented by literature tracing. The search terms covered vocabulary related to CHB, such as depression, anxiety, and risk factors. The literature was selected according to strict inclusion and exclusion criteria. Two researchers independently and blindly screened the literature and extracted key information. STATA12.0 and RevMan 5.3 software were used to perform meta-analysis of the extracted data, and an appropriate model based on the heterogeneity of the studies was selected.RESULTSThe study included 14 articles from five countries (China, United States, Turkey, Australia, and Vietnam), involving 4494 patients with CHB. The analysis revealed that the incidence of comorbid depression in patients with CHB was 30.1% [95% confidence interval (95%CI): 0.182-0.420], the incidence of comorbid anxiety was 40.2% (95%CI: 0.126-0.678), and the overall incidence of negative emotions was 33.7% (95%CI: 0.182-0.420). Further research identified eight factors significantly associated with depression and anxiety. Among them, high educational level was a protective factor against comorbid anxiety and depression in patients with CHB [odds ratio (OR) = 0.43, 95%CI: 0.20-0.93, P < 0.05]. The risk of comorbid depression and anxiety was higher for older patients (OR = 3.05, 95%CI: 1.90-4.90, P < 0.05), longer treatment duration (OR = 3.11, 95%CI: 1.72-5.62, P > 0.05), presence of comorbidities (OR = 2.65, 95%CI: 1.21-5.77, P < 0.05), poor sleep quality (Fisher’s Z = 0.48, 95%CI: 0.41-0.55, Summary r = 0.447, P < 0.05), emotional instability (Fisher’s Z = 0.42, 95%CI: 0.29-0.55, summary r = 0.393, P < 0.05), a high number of hepatitis relapses (OR = 4.07, 95%CI: 2.81-5.89, P < 0.05), and a high degree of hepatitis (OR = 4.28, 95%CI: 1.76-10.41, P < 0.05).CONCLUSIONMultiple factors were significantly associated with comorbid depression and anxiety in patients with CHB. Clinically, it is essential to identify high-risk patients at the earliest opportunity and implement effective intervention to enhance patients’ mental health and optimize CHB disease management and treatment.