- Research Article
1- 10.5363/tits.23.9_74
Views of Industry on the Issues of Digital Sequence Information on Genetic Resources Under the Convention on Biological Diversity
- Sep 01, 2018
- TRENDS IN THE SCIENCES
- Ayumu Inoue
BioIndustry Association
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- 10.1007/s10529-008-9847-6
Construction of plasmids suitable for in vitro synthesis of full-length mRNAs having a 3′-poly(A)+tail
- Sep 23, 2008
- Biotechnology Letters
- Taisuke Matsuo + 8 more +8
In vitro synthesized mRNAs are often used as calibrants in gene expression analysis. In the case of an analytical procedure for gene expression containing a process of reverse transcription such as microarray analysis, full-length mRNAs having a 3'-poly(A)+tail are required as calibrants. However, the preparation of full-length mRNAs having a 3'-poly(A)+tail by using ordinary plasmid vectors is difficult. In this study, we established two plasmid vectors in which the nucleotide sequence corresponding to the 3'-poly(A)+tail were included. By simply inserting full-length cDNAs lacking their 3'-poly(A)+tail into established plasmid vectors, full-length mRNAs having a 3'-poly(A)+tail were successfully prepared.
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