Biotika (Slovakia)

The study of the type material as well as the additional specimens collected in the Rumija Mts., southeastern Montenegro revealed that Bathyscia mikati Udržal, 1995 belongs to the genus Bathyscidius Jeannel, 1910. New combination, Bathyscidius mikati (Udržal, 1995) comb. nov. is established. The species redescription, morphological comparison with related species, revised distribution, the map and the catalogue for the genus are provided.

1Transplant Center, Faculty Hospital Martin, Martin/SLOVAK REPUBLIC, 2Clinic Of Transplant And Vascular Surgery, Martin faculty hospital, Martin/SLOVAK REPUBLIC, 3Biocyt, Banská Bystrica/SLOVAK REPUBLIC, 4Detp Of Radiology, Faculty hospital Martin, Martin/SLOVAK REPUBLIC

Sporulation conditions (sporulation period and the sporulation broth) of the elaiophylin producer Streptomyces melanosporofaciens were optimized. The best culture sporulation was on agar broths with dextrin or hydrolyzed corn starch as carbon sources.

Penicillin G acylase (PGA) from Escherichia coli was cross-linked with mannan dialdehydes. Conjugates were prepared with molecular masses varying from 140 to 580 kDa and containing from 18 to 50% (w/w) saccharides, the values depending on the reaction conditions (mannan/enzyme ratio), and by using mannans with different degrees of oxidation and weight-average molecular mass (M macro(w)). The pH- and thermo-stability of all preparations of glycosylated enzyme were improved remarkably, whereby the influence of the character of the linked mannan dialdehyde, its content, as well as the molecular mass of prepared glycoconjugates, on the stability of PGA, was evaluated. PGA glycosylated with the most oxidized mannan up to an M(w) of 490 kDa, containing 41% (w/w) saccharides, and retaining 90% of its original catalytic activity, showed the highest stability. The half-life of this PGA preparation increased significantly: 13-fold at pH 3, 7-fold at pH 10, and 3.5-fold at pH 8 (all at 37 degrees C), compared with the native enzyme. At higher temperatures (50 degrees C) even more significant stabilization was evident, a 16-fold increase in half-life, from 18 min to 289 min, at pH 8, being measured.