Bioveta (Czechia)

A novel and simple isocratic reversed phase HPLC method with UV and fluorimetric detection for the simultaneous determination of the active anthelmintic components pyrantel pamoate, praziquantel, fenbendazole, its degradation product oxfendazole, and the antioxidant butylhydroxyanisole (BHA) has been developed and validated. The chromatography was performed using a Phenomenex Luna 3 μm phenyl–hexyl column (150 × 3.0 mm), and the mobile phase was composed of 0.5% triethylamine at pH 9.0 and acetonitrile 55 : 45 (v/v) at a flow rate of 1.0 ml min−1. The UV detection was performed at 290 nm for pyrantel, oxfendazole and fenbendazole and at 220 nm for praziquantel. For BHA, fluorescence detection was used with excitation and emission wavelengths of 287 nm and 322 nm, respectively. The method was validated and was applied for the determination of active compounds in various dosage forms of veterinary formulations.

Sera of 204 wild boars (Sus scrofa), shot by hunters in the South-Moravian district of Beclav during 1993-2001, were tested by microagglutination reaction using safranin-stained antigens of Francisella tularensis and Brucella abortus: 10.8% and 8.7% seroreactors, respectively, were detected. The highest (17%) prevalence of tularaemia antibodies was found in wild boars during 1993–1994 at the beginning of a widespread outbreak of tularaemia in South Moravia that started in 1994, a nonsignificantly lower (13%) seroprevalence in 1995–1996 during the continuing epizootic, whereas it decreased markedly to 3% in the years 1997–2001 during the disappearance of the epizootic. Brucella sp. antibodies were significantly most frequent (15%) in wild boars in the years 1995–1996. This Brucella seroreactivity has been attributed to B. suis biotype 2 (B. melitensis biovar Suis biotype 2 according to new nomenclature) infection, because B. abortus in both cattle and humans (Bang’s disease) was eradicated in the former Czechoslovakia in 1964. The hare brucellosis (B. suis biotype 2) has occurred in the Beclav district in a number of natural foci revealing an increased activity since 1994.