Borlaug Institute for South Asia

The green revolution enhanced global food production, ensuring food security for a rapidly growing population. However, overreliance on traditional fertilizers has led to environmental degradation and poor nutrient use efficiency. This necessitates a paradigm shift toward sustainable agriculture. Nanofertilizers are a frontier technology having the potential to overcome such challenges by optimizing nutrient delivery and reducing environmental footprint. This review critically assesses the mechanistic basis of nanofertilizers, beginning with synthesis methods and formulation techniques which enable their controlled and targeted nutrient release. It further discusses, the fate and transformation of nanofertilizers in soil and atmospheric matrices. Also, interaction with microbial populations, along with mechanisms of uptake, translocation, and cellular internalization in plants. The review also explores pivotal areas of nanotoxicology, detailing phytotoxicity mechanisms, defining dose-limiting toxicity thresholds, and deliberation on mitigation options. A brief overview of the developing regulatory schemes is also presented, coupled with the present landscape of innovation and future prospects. Such an integration emphasizes the significance of multidisciplinary approach, linking strong regulatory controls with technological development to safely and efficiently harness nanofertilizers for a sustainable and climate-resilient food future.

The most common cropping production system in South Asia, transplanted puddled rice followed by conventional-tillage wheat, is highly unsustainable, extremely energy-intensive, and emits a large amount of greenhouse gases. The practices used in conservation agriculture, including diversified cropping rotations, residue retention, zero-tillage direct-seeded rice, and zero-tillage wheat, can increase crop productivity while reducing energy use requirements and carbon footprints. Therefore, to promote a sustainable and energy-efficient conservation agriculture-based system with a less energy-intensive rice–wheat system, contrasting tillage and residue management scenarios were evaluated in this study. The treatments include triple cropping systems of zero-tillage direct-seeded rice (ZTDSR) during the rainy season, followed by zero-tillage rice–wheat–mungbean (ZTRWM) in winter, as well as zero-tillage rice–lentil–mungbean (ZTRLM), zero-tillage rice–chickpea–mungbean (ZTRCM), and zero-tillage rice–mungbean–mustard (ZTRMM) along with the conventional-tillage rice–wheat (CTRW) system. Zero-tillage systems exhibited significantly lower operational energy for irrigation (~40%), sowing (~26%), and land preparation (100%) compared to a conventional-tillage (CT) system. Compared to the conventional-tillage rice–wheat system, zero-tillage cropping systems achieved significantly higher system biomass yields. The zero-tillage system also increased wheat yields, resulting in a significant reduction in resources (fuel, fertilizer, and machinery) under zero-tillage (ZT) interventions. More than 60% of energy utilization came from crop residue, irrespective of the diverse cropping production systems. The maximum net energy returns, energy ratios, energy productivity, and energy intensity were recorded with the zero-tillage rice–wheat system. Zero-tillage production systems had significantly lower carbon footprints, higher carbon efficiency, and better carbon sustainability index than the conventional-tillage (CT) management system. Thus, it can be concluded that triple-zero-tillage production systems, along with residue management, yield lower net energy output, greenhouse gas emissions, and carbon footprints as compared to conventional-tillage-based systems.

La canola es un tipo de colza (Brassica napus L. y B. rapa L.), la cual fue mejorada genéticamente en Canadá, se logró que la semilla tuviera menos de 2% de ácido erúcico en el aceite y menos de 30 micromoles de glucosinolatos por gramo de pasta y de esta forma ser aprovechada en la alimentación humana y animal. También, la disponibilidad limitada de agua es un problema recurrente, lo cual obliga a los productores a buscar opciones de cultivos de bajo consumo de agua como la canola. El objetivo del estudio fue identificar variedades y líneas elite de canola de alto potencial de rendimiento, que permita su recomendación a productores para su siembra. La investigación se estableció en el Campo Experimental Norman E. Borlaug-INIFAP, en Ciudad Obregón, Sonora, México. La siembra se realizó el 24 de noviembre de 2020, en el ciclo agrícola otoño-invierno 2020-2021. Se realizó un ensayo de rendimiento de 10 líneas elite y seis variedades de canola; también parcelas demostrativas de cinco líneas elite y cinco variedades de canola. Se establecieron los caracteres agronómicos: inicio de floración (20% de las plantas presentan las primeras flores), madurez fisiológica (80% de las plantas presentan coloración típica) y se registraron en días después de la siembra (DDS); acame, altura de planta, peso hectolítrico (PH) y rendimiento de grano (kg ha-1). Las variedades de canola con mayores rendimientos fueron Centenario, Aztecan y Canorte. Se identificaron líneas elites sobresalientes como SEL-M-F3-445 y SEL-F3-135 que superaron en rendimiento y comportamiento agronómico a las variedades comerciales, por lo que son importantes para fines de liberación de nuevas variedades. Por los rendimientos obtenidos de las diferentes variedades evaluadas, es factible la recomendación para su siembra a los productores de la región de Ciudad Obregón, Sonora.

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Yogurt is one of the most nutritious probiotic food and serves as a medium for nutrients supplementation. The prevalent deficiency of iron and vitamin A within the population prompted the creation of retinol acetate and iron-fortified yogurt. In the yogurt-making process, temperatures typically remain below 100°C for 5 to 10 minutes, and the fortified milk exhibited remarkable heat stability, surpassing even the effects of sterilization treatment (140°C for over 20 minutes). Sensory evaluations of the fortified yogurts yielded scores comparable to the control yogurt. The fortified variety set in a similar time frame, and the quantity of microorganisms used in the inoculation mirrored that of the control yogurt. Acetaldehyde, a key flavoring compound, was produced in a similar manner to the control yogurt (p>0.05). Physico-chemical properties of the fortified yogurt closely resembled those of the control, with improvements seen in viscosity and textural attributes, though these values were statistically similar (p>0.05). The fortified yogurt demonstrated stability along with consistent quality, texture, and sensory appeal, suggesting its potential for commercialization to address nutrient deficiencies.

Various methodologies are used to estimate the impact of changing climatic factors, such as precipitation, temperature, and solar radiation, on crop production and water demand. In this study, the changes in rice yield, water demand, and crop phenology were estimated with varying CO2 concentration and an ensemble of general circulation models (GCMs), using a decision support system for agrotechnology transfer (DSSAT), a crop growth model. The measured CO2 concentration of 400 ppm from the Keeling curve, was used as the default CO2 concentration to estimate yield, water demand, and phenology. These outputs, obtained with the default concentration, were compared with the results from climate change scenarios’ concentrations. Further, the outputs corresponding to the ensembled GCMs’ climate data were obtained, and the results were compared with the ensembled crop model outputs simulated with each GCM. The yield was found to increase with the increase in CO2 concentration up to a certain threshold, whereas water demand and phenology were observed to decrease with the increase in CO2 concentration. The two approaches of the ensemble technique to obtain final outputs from DSSAT results did not show a large difference in the predictions.

Wheat (Triticum aestivum L.) production is adversely impacted by Septoria nodorum blotch (SNB), a fungal disease caused by Parastagonospora nodorum. Wheat breeders are constantly up against this biotic challenge as they try to create resistant cultivars. The genome-wide association study (GWAS) has become an efficient tool for identifying molecular markers linked with SNB resistance. This technique is used to acquire an understanding of the genetic basis of resistance and to facilitate marker-assisted selection. In the current study, a total of 174 bread wheat accessions from South Asia and CIMMYT were assessed for SNB reactions at the seedling stage in three greenhouse experiments at CIMMYT, Mexico. The results indicated that 129 genotypes were resistant to SNB, 39 were moderately resistant, and only 6 were moderately susceptible. The Genotyping Illumina Infinium 15K Bead Chip was used, and 11,184 SNP markers were utilized to identify marker–trait associations (MTAs) after filtering. Multiple tests confirmed the existence of significant MTAs on chromosomes 5B, 5A, and 3D, and the ones at Tsn1 on 5B were the most stable and conferred the highest phenotypic variation. The resistant genotypes identified in this study could be cultivated in South Asian countries as a preventative measure against the spread of SNB. This work also identified molecular markers of SNB resistance that could be used in future wheat breeding projects.