Boryszew (Poland)

Abstract Zoological gardens play a crucial role in the conservation of endangered species. Non-human primates (NHPs) in captivity are exposed to close human contact and various stressors, increasing their susceptibility to numerous diseases, mostly zoonotic. In this study, we conducted a parasitological analysis of primates in five zoological gardens in Poland.Fecal samples were collected from 182 animals, representing 37 NHP species, living in 59 groups. The samples were analyzed using a concentrated smear technique, rapid antigen immunochromatographic tests, and molecular diagnostics targeting protozoa. Additionally, sequencing was conducted on samples that tested positive for Giardia intestinalis.Parasites were detected in 78% of samples. Microscopic examination revealed the presence of nematodes (Trichuris spp., Capillaria spp., Strongylida suborder), cestode (Hymenolepis diminuta), and protozoa (G. intestinalis, Trichostomadidae family, intestinal amoebas, and Blastocystis spp.). Molecular analysis confirmed the presence of genomic DNA from G. intestinalis, Pentatrichomonas hominis, and Entamoeba spp. No Cryptosporidium spp. or Entamoeba histolytica DNA were detected. Sequencing identified G. intestinalis subtype B in six NHPs species. As a result of statistical analysis, it turned out that parasites occurred more frequently in Catarrhini parvorder and in primates with a terrestrial lifestyle.This study presents investigation of intestinal parasites in captive primates in Poland, covering multiple zoological gardens and employing comprehensive molecular diagnostics. Our findings highlight the necessity of regular parasitological screening of primates and further research into transmission pathways and zoonotic potential.

• In-situ surface roughness measurement system (GMS) integrated into the production cell. • GMS with a confocal sensor validated using certified calibration specimens. • Reliable roughness measurements demonstrated under industrial conditions. • Enhanced accuracy through robot path correction and advanced data filtering. The Geometric Measurement System (GMS) was developed and integrated into a robotic production cell for real-time geometric and surface measurements. This article explores the evaluation of the GMS in conducting in-situ roughness assessments using a confocal sensor with a resolution of 0.1 µm and a measuring range of ±5 mm. Surface roughness measurements were performed immediately after turning and milling, extending the system’s capabilities to detect surface quality before final finishing operations. The expansion includes the evaluation of profile roughness parameters ( Ra , Rsk , Rku ) based on ISO 21920-2, real-time verification of surface compliance with defined tolerances, and automatic data archiving in a process history database to support traceability and adaptive corrections. The study demonstrates the system’s effectiveness in analyzing challenging surfaces, including those made of difficult-to-machine alloys like NiTi and geometrically complex components such as turbine blades.

This article aims at a quantitative assessment of the performances of a panel of exchange-correlation functionals, including semilocal (BLYP and PBE), global hybrids (B3LYP, PBE0, M06, BHandHLYP, M06-2X, and M06-HF), and range-separated hybrids (CAM-B3LYP, LC-ωPBE, LC-BLYP, ωB97X, and ωB97X-D), in predicting the vibrationally resolved absorption spectra of BF2-carrying compounds. To this end, for 19 difluoroborates as examples, we use, as a metric, the vibrational reorganization energy (λvib) that can be determined based on the computationally efficient linear coupling model (a.k.a. vertical gradient method). The reference values of λvib were determined by employing the CC2 method combined with the cc-pVTZ basis set for a representative subset of molecules. To validate the performances of CC2, comparisons with experimental data have been carried out as well. This study shows that the vibrational reorganization energy, involving Huang-Rhys factors and normal-mode frequencies, can indeed be used to quantify the reliability of functionals in the calculations of the vibrational fine structure of absorption bands, i.e., an accurate prediction of the vibrational reorganization energy leads to absorption band shapes better fitting the selected reference. The CAM-B3LYP, M06-2X, ωB97X-D, ωB97X, and BHandHLYP functionals all deliver vibrational reorganization energies with absolute relative errors smaller than 20% compared to CC2, whereas 10% accuracy can be achieved with the first three functionals. Indeed, the set of examined exchange-correlation functionals can be divided into three groups: (i) BLYP, B3LYP, PBE, PBE0, and M06 yield inaccurate band shapes (λvib,TDDFT < λvib,CC2), (ii) BHandHLYP, CAM-B3LYP, M06-2X, ωB97X, and ωB97X-D provide accurate band shapes (λvib,TDDFT ≈ λvib,CC2), and (iii) LC-ωPBE, LC-BLYP, and M06-HF deliver rather poor band topologies (λvib,TDDFT > λvib,CC2). This study also demonstrates that λvib can be reliably estimated using the CC2 model and the relatively small cc-pVDZ basis set. Therefore, the linear coupling model combined with the CC2/cc-pVDZ level of theory can be used as a very efficient approach to determine λvib values that can be used to select the most adequate functional for more accurate vibronic calculations, e.g., including more refined models and environmental effects.