Canada Foundation for Innovation

When an isometric contraction is sustained at a submaximal torque, activation of the motoneuron pool increases, making it difficult to interpret neural excitability alterations. Thus, more recently, isometric contractions with maintained electromyographic (EMG) activity (matched-EMG) are being used to induce fatigue; however, little is known about the neurophysiological adjustments that occur to satisfy the requirements of the task. For our study, 16 participants performed a 10-min sustained isometric elbow flexion contraction at 20% maximal voluntary contraction (MVC) torque or the level of integrated biceps brachii EMG recorded at 20% MVC torque. Surface EMG was used to assess global median frequency, and four fine-wire electrode pairs were used to obtain motor unit (MU) discharge rate from biceps brachii. Torque or EMG steadiness was also assessed throughout the fatiguing contractions. MU discharge rate increased and torque steadiness decreased during the matched-torque contraction; however, MU discharge rate decreased during the matched-EMG contraction, and no changes occurred for EMG steadiness. Data pooled for the two contractions revealed a decrease in global median frequency. Lastly, a greater loss of MVC torque was observed immediately after the matched-torque compared with matched-EMG contraction. These findings indicate that, during a matched-torque fatiguing contraction, the nervous system increases MU discharge rates at the cost of poorer steadiness to maintain the requisite torque. In contrast, during a matched-EMG fatiguing contraction, a reduction of MU discharge rates allows for maintenance of EMG steadiness.

Incus necrosis is a common complication following stapes surgery and is associated with impaired microcirculation. The objective of this study was to investigate the vascular anatomy of the human incus by using light microscopy, micro-computed tomography (micro-CT), and synchrotron phase-contrast imaging (SR-PCI) for a novel three-dimensional (3D) analysis of the middle ear, mucosal folds, major vascular pathways, and intraosseous vascular bone channels. One-hundred-and-fifty temporal bones from the Uppsala collection were analyzed under light microscopy. Twenty temporal bones underwent high-resolution micro-CT scanning, and an additional seven specimens underwent SR-PCI at the Canadian Lightsource in Saskatoon, Canada. One of these specimens was from an individual who had undergone stapes surgery. Data were processed with volume-rendering software to create 3D reconstructions using scalar opacity mapping for bone transparency, cropping, and soft tissue analyses. Micro-CT and SR-PCI with 3D rendering revealed the extensive vascular plexus within the un-decalcified incus bone communicating with the exterior surface. The relationship between the vessels, lenticular process, and incudostapedial joint were clearly observed. SR-PCI allowed for histologic-level detail while preserving the specimen and its 3D relationships. SR-PCI with 3D reconstructions confirmed the main vascular supply to the lenticular process along the intraosseous lenticular vessels. This is the first synchrotron analysis of a patient having undergone stapes surgery, and it suggests that incus necrosis associated with stapes surgery may be caused by a disruption of the lenticular blood flow induced by the prosthesis loop, and not by strangulation of mucosal vessels as has been previously described.

Tear meniscus height (TMH) is an indirect measurement of tear film volume. This study investigated the temporal changes in the TMH during the blink interval in the morning (8-9 am) and at the end of the day (5-6 pm) in both soft contact lens (CL) and nonlens wearers (NLW). Fifty participants (25 CL; 25 NLW) were evaluated for their subjective symptoms, TMH, noninvasive break up time, and bulbar hyperemia at the am and pm visits on the same day. The TMH was measured at set intervals between 2 and 15 sec during the blink interval, using an optical coherence tomographer. The NLW group revealed no changes in a variety of symptoms during the day, whereas the CL group reported an increase in dryness (P=0.03) and grittiness (P=0.02) over the day. For both groups, the TMH and calculated tear meniscus volume revealed lower values immediately after the blink and increased progressively afterwards, mainly due to reflex tearing. The am tear meniscus volume values tended to be higher than the pm values for both groups, but this was not significant (NLW P=0.13; CL P=0.82). Noninvasive break up time deteriorated during the day for both groups but was only significant for the CL group (P=0.002), whereas bulbar hyperemia revealed no statistically significant change for either group. Reflex tearing may play a substantial role in the TMH differences observed over the blink interval. Standardization of the time when a TMH measurement is performed will be valuable in comparing tear film clinical studies.

With the drive to cast higher quality, many minimills are adopting mould powder as a lubricant for the continous casting of steel billets. Over the past three decades considerable experience has been accumulated on the relationship between mould behaviour and billet quality for oil lubrication, but comparatively few studies have been conducted for mould powder lubrication. This study, conducted at a Canadian minimill, involved instrumenting four faces of a copper mould with thermocouples and monitoring mould temperatures during casting of 208 × 208 mm billets with mould flux lubrication. Billet samples were also taken to coincide with periods of measurements. Mould temperatures were monitored for two different mould powder compositions, for different mould oscillation frequencies, two mould cooling water velocities, and a range of steel compositions. An inverse heat conduction model was developed to calculate mould heat transfer from the measured temperatures. In this paper, which is the first part of a two part series, details of the inverse heat conduction model and mould heat transfer data are presented. The results obtained for mould flux lubrication have been compared with those for mould heat transfer for oil lubrication. For peritectic steels, with carbon content in the range 0·12–0·14%, it was found that lubricant type has little influence on the measured mould heat flux distribution at the centreline of a face. The peak mould heat flux was found to be approximately 2500 kW m-2 . In contrast, for medium carbon steels, mould heat transfer with mould powder was significantly lower than when oil was employed as a lubricant. For instance, at the meniscus, the peak heat flux with mould powder was approximately 2500 kW m-2 , which was half that recorded with oil as a lubricant. The influence of oscillation frequency, mould cooling water velocity, and mould powder type on mould heat flux has also been presented.