Catholic University in Ruzomberok

Accurate biodosimetry is essential for effective radiological triage, precise clinical monitoring, and assessment of risks from diagnostic exposures. Immunofluorescent detection of phosphorylated histone H2AX (γH2AX) and p53-binding protein 1 (53BP1) DNA double-strand break repair foci provides high sensitivity for radiation dose assessment within the first hours after exposure. However, inter-laboratory reproducibility of γH2AX/53BP1-based biodosimetry remains limited, and the contribution of pharmacological modifiers is unresolved. Here, we conducted an inter-laboratory comparison of in vitro radiation dose-response relationships of foci yields measured in cryopreserved umbilical cord blood lymphocytes (UCBLs) and freshly isolated peripheral blood lymphocytes (PBLs) from healthy donors using fluorescent microscopy with emphasis on workflow harmonization and reproducibility across laboratories. Under low-dose γ irradiation, both UCBLs and PBLs exhibited a strong linear, dose-dependent induction of γH2AX, 53BP1, and co-localized foci, with co-localization emerging as the most sensitive endpoint. γH2AX pan-nuclear staining was observed exclusively in UCBLs and functioned as a distinct endpoint under the examined conditions. Under harmonized low-dose conditions, calyculin A at a non-toxic concentration of 1 nM did not provide measurable stabilization or enhancement of ionizing radiation-induced foci (IRIF) yields. Although IRIF yields differed between the two laboratories, dose-response slopes were highly concordant, demonstrating reproducibility under harmonized experimental conditions. These findings demonstrate that inter-laboratory reproducibility of γH2AX/53BP1-based biodosimetry is achieved primarily through disciplined workflow harmonization rather than through pharmacological enhancement. By reinforcing assay reproducibility and biological consistency, this work supports the translational applicability of IRIF-based biodosimetry for broader application in radiation exposure assessment.

Previous research has explored the impact of corrective feedback provided by human instructors on the writing skills development of ESL/EFL (English as a second/foreign language) learners. There also has been a growing trend towards employing corrective feedback generated by Meta AI for the same pedagogical purposes. However, no studies to date have examined the effect of such feedback on reducing grammatical errors in English writing among language learners., Therefore, the present study aims to examine the effects of three distinct types of corrective feedback, direct, indirect, and metalinguistic generated by Meta AI in conjunction with the WhatsApp mobile application, on grammatical errors in the written English of first-year undergraduate university students. The study employed random sampling to select four sections of undergraduates, comprising a total of 227 students. Three sections were assigned as the experimental group and one as the control group. Section A (N=59) received direct corrective feedback, Section B (N=53) received indirect corrective feedback, Section C (N=58) received metalinguistic corrective feedback, and Section D acted as the control group (N=57). Pictures were given to the students to compose a story in English, totalling 300 words, to collect data from them through a pretest and post-test. The data were analysed in terms of morphological, syntactic, and orthographic errors, employing the theoretical framework of Corder’s (1974) and Dulay’s (1982) taxonomies. The frequency of errors was recorded, and a repeated-measures ANOVA (analysis of variance) was used to analyse the data in SPSS (version 26). The statistical analysis revealed that the mean of errors decreased in the post-test writings of each section. Significantly, the metalinguistic corrective feedback produced by Meta AI proved effective in reducing errors compared to both direct and indirect feedback methods, in addition to the control group. This study suggests that integrating AI-generated metalinguistic feedback into English learning and teaching curricula could enhance error correction and learning outcomes in higher education.

The study focuses on the growing phenomenon of parent sharenting. The research data was obtained by using a structured interview method. The research population comprised 11 respondents, ten females and one male aged AM=35 years. All respondents are parents of children between 2 and 25 years old, who use social networking sites and have at least once shared content about their children or adolescents in an online social networking environment. The research is unique in its focus, as there is a lack of research on the topic in Slovakia. The results of our research have shown that the age of parents does not influence the frequency or form of sharenting. The research results suggest that parents are mainly guided by personal preferences when sharing content about their children. Most of the parents consider sharing content about their children on social media a natural part of their lives. We found that parents are concerned about not knowing who can access the shared content. The risks associated with sharing content about children on social media perceived by parents are mainly misuse of photos and child abduction. Most parents inform their children about what content they want to share about them.

Children and young people’s use of the Internet inevitably involves the possibility of encountering what we collectively refer to as “harmful” or “toxic” content. In our research sample, approximately 40% of respondents reported such experiences. One prominent category of harmful content includes material promoting extreme thinness, including pro-anorexia and pro-bulimia websites. Among all respondents, 5% encountered such content daily, with a significant gender difference—9% of girls compared with 1% of boys. The likelihood of exposure also increased with age. Young Internet users experiencing emotional difficulties or a strong tendency to seek out novel and risky experiences reported more frequent exposure to harmful content. While an accepting family environment appears to be a possible protective factor, the low correlation suggests this influence is limited.

Aim. This research investigates the effectiveness of constructivist-based learning in developing the writing skills of EFL students. Methods. A sample of 60 second-year students from the Department of English Language at “Aleksander Moisiu” University was selected. The sample was divided into two groups, an experimental group (n=30) and a control group (n=30), on the basis of English proficiency levels. The instruments used for this study include an EFL writing skills test (pre- and posttest) and the Writing Quality Scale (Stuart and Barnett, 2024) for scoring the written essays. The control group received instruction through a traditional teaching method, whereas the experimental group was taught in a constructivist learning environment. The analysis focused on the integration of new linguistic input into students’ writing tasks, assessing the lexical, grammatical, and textual elements they utilised to enhance their writing techniques. Results. A comparison of the scores between the two groups revealed statistically significant differences in favour of the experimental group in terms of EFL writing skills. The findings suggest that students' writing skills improved over time with the implementation of the constructivist approach. Conclusion. This study views the constructivist approach as a successful framework for teaching writing skills. However, the study’s limitations should be addressed, and other areas of learning should be explored to further verify the positive effects of constructivism in language education.

Abstract This article is intended to be an interpretation to the question of truth in Kierkegaardian writings under the pseudonym of Johannes Climacus (mainly from Concluding Unscientific Postscript), and intended to present, in accordance with his insightful considerations of his time, the most essential points of what might be called a Kierkegaardian diatribe addressed to modernity. At the same time, his ideas are confronted with other thinkers in a dialectical way in order to contrast them and to delimit their meaning. The distinguished Dane suggests surreptitiously, but also directly, a vindication of what is human based on a search for Christian truth, decision-making, the passion to exist, intensive spirituality, and the convictions that give meaning to human existence. All this in the face of the characteristics of modernity, such as excessive rationality, reflection, scientific objectivity, abstract subjectivity and, in general, intellectual confusion with respect to the categories proper to the Christian religion

Abstract This study presents a novel experimental platform designed to systematically investigate the effects of low-frequency (LF) magnetic fields (MFs) on biological systems. By overcoming key methodological challenges, including variability in environmental conditions and poor reproducibility, this platform sets a new standard for experimental reliability. It integrates precise temperature regulation, high MF homogeneity, and a modular structure, that ensures adaptability to diverse experimental setups. The platform was validated using Saccharomyces cerevisiae CCY 21-4-99 as a model organism, cultivated under controlled conditions with and without MF exposure. The minimal growth variations observed between chambers confirm the ability of the platform to maintain reproducible conditions and support statistically robust experimental designs. The platform can be applied to diverse biological systems and can be technically adapted to different experimental requirements, including different electromagnetic field sources. It provides a highly controlled environment for investigating the cellular and subcellular effects of LF MF, creating a solid foundation for future research into its biological mechanisms and potential applications.

The elite swimmer of the Slovak Republic (T.P.), a participant in the 2023 World Swimming Championships in Fukuoka, has since 2012 recorded a gradual increase in performance in the swimming disciplines of 400 m freestyle with a resulting time of 3:49.03 (795 points, SCM) and 800 m freestyle with a resulting with a time of 8:12.87 (771 points, LCM). From the point of view of the annual training macrocycle of ATC 2023, he achieved his best sports performances in the 400m freestyle with a final time of 3:53.88 (747 points, SCM) and in the 800m freestyle with a fi-nal time of 8:07.68 (751 points, SCM). We identified the general and special training variables that were most closely related to the swimmer's athletic performance through a retrospective analysis. For interaction identification and prediction, we applied non-parametric techniques (CHAID de-cision trees and Spearman's correlation coefficient). The available data was analyzed using the technique to examine at the swimmers' workout records from the ATC 2023 annual training cycle. The available data, which include the volume of race pace (STI 105), elemental arm swimming (STI 106), and volume of anaerobic endurance (STI 104), as well as the number of starts (GTI 112) and training units (GTI 111), were analyzed by looking at the swimmers' training diaries from the ATC 2023 annual training cycle.

Introduction: Type 2 diabetes is a chronic disease that contributes to the occurrence of limitations in the patient's life. In order to assess the quality of life of patients with diabetes, the following aspects should be taken into account: age, gender, education, presence of complications, Material and methods: The study was conducted using the diagnostic survey method. The research tools were: a survey questionnaire consisting of metrics and clinical parameters, the ADDQoL questionnaire, The study was conducted on a group of 104 patients (including 58 women and 46 men) in the Diabetes Clinic at the Provincial Hospital in Bielsko-Biała in the period from April 20, 2018 to September 1, 2018. Results: Diabetes has been shown to have a negative impact on all 19 ADDQoL domains. The most negative feelings concern the future, freedom in eating, and enjoying leisure activities. Conclusions: Patients with type 2 diabetes have a negative impact on their quality of life. Patients with diabetes require greater focus on educational activities aimed at adhering to a rational diet and psychological support.