Fujian Blood Center

To explore the application value of reticulocyte hemoglobin equivalent (Ret-He) for diagnosing latent iron deficiency in female plateletpheresis donors. A total of 230 female plateletpheresis donors in Fujian Blood Center from January to February 2022 were selected as the research group and divided into three groups: normal group, iron depletion (ID) group and iron deficient erythropoiesis (IDE) group, according to the severity of iron deficiency. The level of hemoglobin (HGB), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), coefficient of variation of red cell distribution width (RDW-CV) and Ret-He were measured by using the Sysmex XN automated hematology analyzer. Chemiluminescence immunoassay was used to detect iron biochemical indexes. Receiver operating characteristic(ROC) curve analysis was performed to evaluate the diagnosic value of relevant indicators in female blood donors with latent iron deficiency. Ret-He in ID group was 32.55 (31.15,33.10)pg, which was significantly lower than that in the normal group ［33.80(32.73，34.70)pg］ （P <0.05）, and significantly higher than that in IDE group ［30.40(28.70,31.50)pg］ （P <0.05）. ROC analysis in diagnosis of IDE demonstrated that the area under the curves (AUCs) of HGB, MCV, MCH, RDW-CV and Ret-He were 0.892, 0.843, 0.909, 0.890, 0.931, respectively. When the critical value of Ret-He was 32.05 pg, its sensitivity and specificity were 85.90% and 92.60%, respectively. However, all red blood cell parameters had poor diagnostic value for ID. Ret-He is a perfect predictor for latent iron deficiency in female blood donors. Detection of Ret-He can advance the diagnosis of iron deficiency in female blood donors to the IDE stage.

To investigate the correlation of iron metabolic parameters with platelet counts in blood donors. A total of 400 blood donors who met requirements of apheresis platelet donation were collected, and their hematological parameters were analyzed. The donors were divided into low ferritin group and normal group, the differences of hematological parameters between the two groups were compared, and the correlation of iron metabolic parameters and routine hematology parameters with platelet counts were analyzed. Whether male or female, low ferritin group had higher platelet counts than normal group (P < 0.01). Among the iron metabolic parameters, the platelet counts was negatively correlated with serum ferritin (SF), serum iron (SI), and transferrin saturation (TSAT) (r =-0.162, r =-0.153, r =-0.256), and positively correlated with total iron binding capacity (TIBC) and unsaturated iron binding capacity (UIBC) (r =0.219, r =0.294) in female blood donors. Platelet counts was also negatively correlated with SF, SI and TSAT (r =-0.188, r =-0.148, r =-0.224) and positively correlated with UIBC (r =0.220) in male blood donors. Among the routine hematology parameters, platelet counts was negatively correlated with mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), and reticulocyte hemoglobin equivalent (Ret-He) in female blood donors (r =-0.236, r =-0.267, r =-0.213, r =-0.284). Platelet counts was also negatively correlated with MCH, MCHC and Ret-He in male blood donors (r =-0.184, r =-0.221, r =-0.209). In blood donors with low C-reactive protein level, the lower the iron store capacity, the lower the iron utilization, and the platelet counts tends to rise.

Abstract Endometriosis (EM) is a chronic gynecological disorder that causes infertility and chronic pelvic pain. The aim of the current study was to identify markers of efferocytosis with utility for EM diagnosis.RNA sequencing profile and single-cell sequencing (scRNA-seq) data were collated from the Gene Expression Omnibus (GEO) database and 46 efferocytosis-related genes (ERGs) from Genecards. Results of single-cell, differential expression and Weighted Gene Co-expression Network Analysis (WGCNA) were combined into a Venn diagram to identify 41 intersecting genes. LGALS2, EGR1 and CLINT1 were shown to be key EM markers by least absolute shrinkage and selection operator (LASSO) and support vector machine-recursive feature elimination (SVM-RFE) algorithms. Area under the curve (AUC) values were 0.9 for LGALS2, 0.81 for EGR1 and 0.76 for CLINT1, indicating good diagnostic efficacy. Functional annotation analysis revealed the markers to be enriched in cell cycle, DNA repair, neuroactive ligand-receptor interactions, cell cycle, chromosomal segregation and other pathways. Drug-gene interaction network indicated that beta-D-glucose, pseudoephedrine and fostamatinib were potential therapeutic agents, exposing the possibility of personalized medicine for EM. RT-qPCR showed LGALS2 and EGR1 to be more highly expressed in ectopic than in eutopic endometrium. LGALS2 and EGR1 are introduced as potential novel targets for risk prediction, non-invasive diagnosis and health care personalization in EM. The potential for personalized medicine (PPPM) to treat EM patients is illuminated.

BackgroundSyphilis infections among volunteer blood donors increased rapidly in recent years. It is important to analyze the demographics of seropositive donor groups and help to recruit donors from low-risk population.ObjectiveThe aim of this study was to analyze the syphilis prevalence among volunteer blood donors in Jinan Blood Center and give direction to blood recruitment.Methods and MaterialsA cross-sectional study was conducted among blood donors in Jinan, China. Socio-demographic data and blood donation testing data from January 2007 to December 2021 were extracted from the database of blood management software of Jinan Blood Center for analysis. All blood samples were screened by ELISA, and those anti-TP-positive samples were counted and analyzed by sex, age, educational background, occupation and blood donation times. Logistic regression was used to explore risk factors associated with syphilis infection.ResultsTotally 700,757 blood samples were collected in the study during 2007 to 2021, 2290 cases were detected anti-TP positive with a positive rate of 0.33%. Female, 35–44 years old, with a lower education degree, farmers and first-time donors were the high-risk subgroups.ConclusionConsultation and identification of high-risk population groups should be improved. Measures should be taken to make the donor recruitment more professional and detailed.

To explore the effect of storage time on arginase level, and the possible source of arginase in suspended red blood cells (RBC). The arginase and myeloperoxidase (MPO) levels in suspended RBC and control plasma were detected by ELISA. The free hemoglobin level in suspended RBC and control plasma were detected by colorimetric method. The relationship between arginase level, MPO level and free hemoglobin level in suspended RBC was analyzed by the related methods. The arginase and free hemoglobin levels in suspended RBC were higher than those in control plasma. Otherwise, MPO level was not significantly different between suspended RBC and control plasma. All of them did not increase along with prolonging of storage time. There was not a significant correlation between arginase level and free hemoglobin level in suspended RBC of different storage time (r = 0.03), but arginase level positively correlated with MPO level in the suspended RBC of different storage time (r = 0.76). The arginase level in suspended RBC storaged for different time increases significantly, but not along with prolonging of storage time. The main possible source of arginase in the suspended RBC is the residual white blood cell, especially neutrophils.

多药耐药(multidrug resistance,MDR)是白血病化疗失败的主要原因之一,已成为白血病化疗及白血病患者长期无病生存的主要障碍.其机制复杂,涉及多方面因素.对多药耐药相关蛋白基因(multidrug resistance-associated protein gene, MRP) 的研究已有较多的报道[1-3].MRP家族已被证实有7个成员MRP1～ MRP7[4].其中关于MRP1研究较多 .MRP2的作用是转运谷胱甘肽(GSH)结合物和化疗药物,比如顺铂、足叶乙甙以及长春新碱;MRP3的作用是泵出氨甲蝶呤和足叶乙甙而非GSH.有关MRP2和MRP3在急性白血病中表达及其与MDR的关系,国内外研究报道较少.本文就急性白血病患者MRP2和MRP3的表达情况作如下报道。