GeneTiCA (Czechia)

Insulin-degrading enzyme (IDE) is a protein with proteolytic and non-proteolytic functions that regulates glucose homeostasis. In the fasted state, glucagon regulates glycemia through induction of hepatic gluconeogenesis. The rate of hepatic gluconeogenesis is elevated in subjects with type 2 diabetes (T2D) compared with healthy subjects. Interestingly, subjects with T2D show decreased expression of hepatic IDE. However, the role of IDE on the regulation of hepatic gluconeogenesis is completely unknow. We hypothesize that IDE deficiency alters glucagon signaling and thereby gluconeogenesis. To test this hypothesis, we used mouse liver tissues and cultured hepatocytes with total or partial IDE deficiency. The glucagon signaling pathway, expression of gluconeogenic genes, glucose production, and transcriptomic analysis were performed in control and IDE-KO hepatocytes. Total or partial loss of IDE in liver tissues or cultured mouse hepatocytes resulted in lower levels of the glucagon receptor (GCGR) and the cAMP-response element binding protein (CREB). However, glucagon stimulation increased the phosphorylation of CREB, despite lower levels of cAMP in IDE-deficient mouse hepatocytes. The activation of CREB was associated with an upregulation of the gluconeogenic genes Pck1 and G6pc (~ 200% and ~ 70% respectively) and higher glucose production in IDE-deficient mouse hepatocytes. Finally, genetic depletion of IDE in HepG2 hepatocytes led to upregulation of genes involved in cellular functions related to membranes, organelles and signaling receptors. These findings may be of relevance to better understand the regulation of hepatic gluconeogenesis and the use of IDE as a potential therapeutic target for the treatment of T2D.

The study of the uterine microbiome through the identification and characterization of the study about uterine microbiome through the identification and characterization of microorganisms allows advances in understanding the local microbiota and, from that, makes it possible to improve the cows reproductive efficiency.The uterus was considered a sterile environment for a long time, current research has shown there is a dynamic local microbiota composed of a great diversity of microorganisms such as bacteria, protozoa and fungi.The uterine microbial composition varies due to several factors like the phase of the estrous cycle, number of calvings, age, breed, genetics, stress, gestational period, puberty, nutrition, dystocia, assistance during birth, presence of contamination, season, accommodation, systemic diseases and, mainly, inflammatory diseases in the postpartum period, classified as metritis, subclinical endometritis and clinical endometritis.Healthy uteruses have higher bacterial diversity in gender and species when compared to clinical and subclinical endometritis.24 genders of bacteria were isolated in the uterus of healthy animals, the most common being Staphylococcus, Escherichia and Bacillus.In cows with clinical endometritis, 22 genders were found, the main ones being Trueperela Escherichia and Staphylococcus.And only 12 bacterial genders have been identified in cows with subclinical endometritis: Bacillus, Enterococcus, Pseudomonas.Uterine diseases can cause a decrease in milk production, fertility redution, discarding of milk and animals and thus, the uterine microbiome comprehension, as well as the impact of bacteria on the development of metritis and endometritis, is necessary to improve the cows reproductive efficiency.

A parceria de estudantes da Universidade Federal do Ceará- Campus Sobral, membros da Sociedade de Genética Médica de Sobral (SAGEMS) com o Centro de Hemoterapia do Ceará (Hemoce), permitiu a participação desses estudantes de medicina nos ambulatórios de hematologia. Nesse contexto, durante o estágio, eles exploraram a infraestrutura do local e participaram ativamente das consultas médicas. Portanto, este trabalho consiste no relato da experiência desses acadêmicos nessa instituição.

modernizao da agricultura e do abandono de atividades agrcolas que acabam levando a diminuio de diversidade gentica.Portanto, este trabalho visa identificar os principais artigos cientficos

primers foram testadas em plantas de tabaco.No avano dessa pesquisa, realizou-se a busca em bancos de sequncias genticas, anlise in silico, testes de bancada como PCR, eletroforese, clonagem, clivagem, agroinfiltrao e sequenciamento.Os resultados para o silenciamento do gene-alvo se demonstraram positivos e corroboram com outros autores.

Very few cases of constitutional interstitial deletions of the proximal short arm of chromosome 3 have been reported; however, the proximal 3p deletion is emerging as a clinically recognizable syndrome. We present an intrachromosomal insertion of 3p12.3p14.1 in a phenotypic normal man (46,XY,ins(3)(p25p12.3p14.1)) which is responsible for the unbalanced karyotype in 2 affected offspring, one with a 3p12.3p14.1 interstitial deletion and the other with a reciprocal duplication. The exceptionality of these 2 reciprocal recombinants contributes to a better definition of the proximal 3p deletion syndrome and its duplication counterpart.

Americanae nace como un proyecto conjunto que surge dentro de la Red Europea de Información y Documentación sobre América Latina (REDIAL), y que ha afrontado la Biblioteca de la Agencia Española de Cooperación Internacional para el Desarrollo (AECID). Esta nueva biblioteca virtual hace más accesibles los libros digitales de tema americanista a los investigadores y usuarios interesados de cualquier parte del mundo.

Mudancas no padrao de expressao das moleculas de adesao sao observadas no desenvolvimento tumoral em diferentes tipos de câncer, estando relacionadas com invasao e metastase. O objetivo deste estudo foi analisar e comparar os padroes de expressao de a- e b-cateninas, E- e N-caderinas em tumores malignos e benignos do epitelio ovariano de superficie, visando estabelecer possiveis correlacoes entre a expressao destas moleculas, o estadio tumoral e a manifestacao de metastases, nas neoplasias malignas. Preparacoes de imunoistoquimica foram obtidas a partir de 88 biopsias (39 benignas e 49 malignas) incluidas em parafina, procedentes de mulheres submetidas a cirurgia para retirada dos ovarios no periodo de 1993 a 2001, no hospital da Universidade Estadual de Campinas. Estas foram avaliadas segundo o numero de celulas positivas, as estruturas celulares e a intensidade da reacao para cada uma das moleculas analisadas. Os resultados mostraram que a maioria dos tumores malignos apresentou reacoes imunoistoquimicas de maior intensidade (p=0,01) para b-catenina, alem de maior numero de celulas positivas para a-catenina (p=0,04). Os carcinomas endometrioides apresentaram expressao aumentada de b-catenina, tanto em numero de celulas positivas (p=0,04) como tambem na intensidade das reacoes (p=0,01). Os tumores malignos onde a marcacao para E-caderina manteve-se preservada na membrana corresponderam aos casos que nao evoluiram para metastases, enquanto os tumores malignos que apresentaram expressao nula ou restrita ao citoplasma, para esta proteina, evoluiram como recidivas da doenca (p=0.04). Considerando estes dados, a E-caderina pode ser um bom marcador de prognostico para câncer de ovario. Paralelamente, a expressao de b- e a-cateninas aparece estatisticamente aumentada nos tumores malignos, sugerindo a possivel utilizacao destes como marcadores discriminantes de malignidade. Quanto a N-caderina, os dados mostraram que a expressao desta esta relacionada mais precisamente ao tipo histologico dos tumores, do que com o estadio tumoral ou evolucao dos casos Abstract

Several parameters of the reproductive function were studied in albino rats of the Wistar strain fed an essential fatty acid-deficient (EFAD) diet, a linoleic acid-sufficient (EFAS) diet or a commercial diet (CD). The frequency of irregular estrous cycles, with an increased incidence of prolonged diestrus, was significantly higher in EFAD and EFAS groups than in the CD group. There were no significant differences between EFAD and EFAS rats in any of the estrous cycle parameters studied. The EFAD rats showed the following changes in reproductive performance in respect to EFAS and CD dams: prolonged period of gestation, reduced litter size and increased pup mortality during delivery and the first 48 neonatal hours. From this time to weaning, EFAD and EFAS pups had a similar rate of mortality and were different in this respect from the CD group. These results support previous reports indicating that linoleic acid is essential to the maintenance of normal pregnancy and the survival of newborn rats. Nevertheless, we cannot conclude that impairment of the estrous cycle and sucking mortality until weaning were primarily due to the absence of linoleic acid in the maternal diet. The possible role of alpha-linolenic acid in the reproductive is discussed.