Gruppo Scienzia Machinale (Italy)

Polyhydroxyalkanoates (PHAs) are microbially produced aliphatic polyesters investigated for tissue engineering thanks to their biocompatibility, processability, and suitable mechanical properties. Taking advantage of these properties, the present study investigates the development by 3D printing of bacterial poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) scaffolds loaded with β-tricalcium phosphate (β-TCP) for bone tissue regeneration. PHBV blending with poly(lactide-co-glycolide) (PLGA) (30wt%) was exploited to enhance material processability via an optimized computer-aided wet-spinning approach. In particular, PHBV/PLGA blends were loaded with different β-TCP percentages (up to 15wt%) by suspending the ceramic particles into the polymeric solution. The composite materials were successfully fabricated into 3D scaffolds with a fully interconnected porous architecture, as demonstrated by scanning electron microscopy. The ceramic phase had a significant effect on PHBV crystallization as shown by differential scanning calorimetry, as well as on scaffold mechanical properties with a significant increase of compressive modulus in the case of 5wt% β-TCP loading. In addition, in vitro cell culture experiments demonstrated that β-TCP loading led to a significant increase of the viability of human mesenchymal stem/stromal cells grown on the scaffolds. Taken together, our data suggest that microbial PHBV processability, biomechanical performance, and bioactivity can be improved through combined PLGA blending and β-TCP loading.

Tissue engineering represents an advanced therapeutic approach for the treatment of bone tissue defects. Polyhydroxyalkanoates are a promising class of natural polymers in this context thanks to their biocompatibility, processing versatility, and mechanical properties. The aim of this study is the development by computer-aided wet-spinning of novel poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)-based composite scaffolds for bone engineering. In particular, PHBV scaffolds are loaded with hydroxyapatite (HA), an osteoinductive ceramic, in order to tailor their biological activity and mechanical properties. PHBV blending with poly(lactide-co-glycolide) (PLGA) is also explored to increase the processing properties of the polymeric mixture used for composite scaffold fabrication. Different HA percentages, up to 15% wt., can be loaded into the PHBV or PHBV/PLGA scaffolds without compromising their interconnected porous architecture, as well as the polymer morphological and thermal properties, as demonstrated by scanning electron microscopy, thermogravimetric analysis, and differential scanning calorimetry. In addition, HA loading results in increased scaffold compressive stiffness to levels comparable to those of trabecular bone tissue, as well as in higher in vitro MC3T3-E1 cell viability and production of mineralized extracellular matrix, in comparison to what observed for unloaded scaffolds. The observed mechanical and biological properties suggest the suitability of the developed scaffolds for bone engineering.

Abstract The development of tailored additive manufacturing (AM) approaches is resulting in novel strategies for engineering the macro/microstructural details of potential medical devices made of biocompatible polymers. The aim of this work is the employment of a multifunctional AM machine for fabricating poly(methyl methacrylate) (PMMA) constructs with a predefined shape and porous architecture, by either melt‐ or solution‐extrusion AM. In particular, PMMA porous scaffolds are manufactured by employing fused filament fabrication (FFF) or computer‐aided wet‐spinning (CAWS). The comparative characterization of the two developed PMMA implant prototypes demonstrates significant differences in terms of porous structure, polymer surface topography, material composition, glass transition temperature, and mechanical properties. As a consequence, FFF‐printed PMMA samples support a faster in vitro proliferation of a murine fibroblast cell line in comparison to PMMA scaffolds by CAWS. The results achieved encourage further research on the developed processing protocols, aimed at the development of tailored PMMA implants for personalized surgery applications.

The recent close approach of comet C/2020 F3 (NEOWISE) allowed us to study the morphology of its inner coma. From the measurement of the dust ejection velocityon spiral structures expanding around the nucleus, we estimated a mean deprojectedexpansion velocity Vd= 1.11+/-0.08 km s^-1. Assuming that a new shell formed after every rotation of the comet, a rotation period of 7.8+/-0.2 hours was derived. The spin axis orientation was estimated at RA 210+/-10d, Dec. +3+/-10d. The comamorphology appears related to two strong, diametrically opposite emissions located at mid-latitudes on the nucleus. A qualitative modelling of the coma produced consistent results with a wide range of dust sizes (0.80 to 800 micro-m), with inversely correlated densities (0.003 to 3.0 g cm^-3). Images taken with Vj and r-Sloan filters showed a greater concentration of dust in the first two shells, and an increasing density of radicals emitting in the B and V band-passes from the third shell outwards. Striae-like structures in the tail suggest that dust particles have different sizes.

ABSTRACTElectromagnetics (EM) has been used extensively for Volcanic Hosted Massive Sulphide (VHMS) exploration in Australia. Exploring under conductive cover introduces significant limitations when using EM to identify bedrock conductivity anomalies that may be associated with VHMS deposits. We present an alternative approach, whereby robust geological modelling of the Airborne Electromagnetic (AEM) data plays a major role in the exploration strategy in the Bryah and Yerrida Basins of central Western Australia. The AEM is not only used for the identification of bedrock conductors but also forms a critical dataset constraining a robust basin-wide geological model. This model is used to identify priority areas for follow up surface geophysics and geochemistry. A patchwork of AEM surveys, covering portions of the Bryah and Yerrida basins, has been acquired by various explorers and contractors during the last decade. Systems and system specifications vary greatly. Accordingly, accurate geological interpretation of a basin-scale area, flown using various systems, cannot be derived from either raw data or fast/approximate conductivity products provided by contractors. All datasets require reconciliation with a common workflow and robust modelling strategy. Historic AEM data acquired with different systems along the edges of the tenure have been reprocessed and inverted. The remaining central block awaits the contractor's arrival before the data is subject to the same workflow. The end result will be a seamless basin-wide 3D conductivity model (extending over 6500 km2), which will inform the geological interpretation and subsequent follow-up exploration efforts. The preliminary 3D models already allow clear identification and modelling of the pyritic shale horizons, enabling the anomalous geochemistry and strongly conductive nature of these units to be discounted in the targeting process

A new integrated system consisting of a testing machine, denominated Diaptometro, and of an algorithm for metallic materials identification via instrumented spherical indentation testing is presented. The identification is carried out by analyzing the experimental load – penetration depth curve ( curve) from shallow indentation tests, thus preventing any contact condition effects in the material properties estimation. Accuracy in the experimental data collecting and the curve representation were recognized to be the key factors at the basis of reliable and accurate estimations. A new equipment with accuracies of 0.075% and 0.1% for the penetration depth and indentation load respectively was specifically built-up, whereas a four-terms power expansion is proposed for modeling the curve in the identification procedure. Experimental validation campaigns confirm the effectiveness of the adopted solutions.

Kinematic control of robot manipulators requires that joint mechanical limits are taken into account in order to avoid the interruption of the task at hand if joint limits are reached. A novel approach to this problem is presented and compared with state of the art techniques. The proposed control scheme allows to explicitly include in the specification of the task position, velocity and acceleration costraints for the joints. An application to an existing redundant arm is discussed and experimental results are presented.