Guangzhou Eighth People's Hospital

The influence of preoperative psychological profiles on weight loss following sleeve gastrectomy (SG) has not been thoroughly examined, particularly with respect to sex differences. To analyze the preoperative psychological profiles of individuals with obesity and explore whether sex differences exist in the associations between preoperative psychological factors and weight loss after SG. This prospective cohort study involved participants scheduled for SG at an affiliated hospital between November 2020 and September 2022, with a follow-up period of 1-year post-surgery. Preoperative psychological profiles were assessed using validated scales, covering anxiety, depression, self-esteem, internalized weight bias, eating behaviors, sleep quality, and quality of life. Independent t-tests and Pearson correlation analyses were used to assess sex differences and associations between psychological factors and %TWL at 1-year post-surgery. Univariate and multivariate regression analyses were then performed to identify independent predictors of %TWL at 1-year post-surgery, with significance defined as p < .05. In total, 491 patients with obesity undergoing SG were enrolled, of whom 452 (314 women and 138 men) completed the study. Preoperatively, participants exhibited high levels of internalized weight bias, moderate alterations in eating behaviors, and poor sleep quality and quality of life. Compared to women, men demonstrated significantly lower levels of anxiety, depression, internalized weight bias, and emotional eating, but demonstrated significantly higher health transition scores (i.e., self-perceived improvement in general health compared to one year ago). Among women, 1-year postoperative weight loss was significantly positively associated with preoperative anxiety and internalized weight bias, but negatively associated with self-esteem and quality of life. Conversely, no statistically significant associations were found between preoperative psychological factors and weight loss outcomes in men. In multivariate regression analysis, no psychological factors independently predicted %TWL at 1-year post-surgery in women. Sex differences were observed in the associations between preoperative psychological factors and weight loss after SG. Among women, psychological factors demonstrated stronger associations with weight loss after SG; however, none of these remained significant predictors in the multivariate regression model. No such associations were observed in males. These findings suggest that the impact of preoperative psychological factors on postoperative weight loss may be complex and merit further investigation.

Abstract Dengue fever, an acute Aedes ‐borne infectious disease caused by the dengue virus (DENV), is a substantial global health challenge, necessitating the development of swift and effective surveillance methodologies. We have developed a Recombinase Aided Amplification (RAA)–Cas12a assay that exhibits superior analytical sensitivity compared with real‐time PCR while maintaining comparable specificity. In clinical specimen testing, the assay demonstrated a sensitivity of 92.3% and a specificity of 100%, using a signal‐to‐noise ratio of ≥2.0 as the positivity threshold. Furthermore, the RAA–Cas12a assay displayed remarkable sensitivity in detecting DENV in both adult and larval mosquitoes, with field data from mosquito surveillance aligning closely with the emergence of local dengue cases. Characterized by its simplicity, rapidity, and precision, the RAA–Cas12a approach emerges as a highly promising technology for monitoring virus carriage in field‐collected mosquitoes, thereby functioning as both an early warning system and a crucial indicator for effective dengue outbreak management. The practicality and cost‐effectiveness of this methodology provide a robust foundation for the establishment of an efficient global collaborative surveillance and risk assessment platform to mitigate the rapid spread of dengue.

Introduction: This study investigated the role of FCGR3A in pediatric inflammatory bowel disease (IBD) through integrated transcriptomic analysis and experimental validation, aiming to provide new mechanistic insights and therapeutic strategies. Methods: Transcriptomic datasets of pediatric IBD were obtained from the Gene Expression Omnibus database, and differentially expressed genes (DEGs) were identified. Weighted gene co-expression network analysis (WGCNA) was applied to screen disease-related modules, followed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses of overlapping DEGs. Hub genes were determined using protein-protein interaction networks, least absolute shrinkage and selection operator regression, and random forest algorithms. Their expression was verified by meta-analysis across multiple datasets, and diagnostic value was assessed using receiver operating characteristic (ROC) curves. The relationship between hub genes and immune infiltration was further explored. Functional assays included lipopolysaccharide (LPS)-stimulated intestinal epithelial and THP-1 macrophage models, as well as a dextran sulfate sodium (DSS)-induced murine colitis model. Results: WGCNA revealed 370 module genes associated with pediatric IBD, enriched in inflammatory pathways. Seven candidate genes were identified, among which FCGR3A showed a strong association with pediatric IBD. Immune infiltration analysis demonstrated that FCGR3A expression correlated with M1 macrophage enrichment. In vitro, FCGR3A was upregulated in LPS-induced epithelial cells, and its knockdown inhibited M1 macrophage polarization. In vivo, FCGR3A was highly expressed in DSS-induced murine colitis, promoting M1 polarization and disease progression. ROC analysis indicated strong predictive value (AUC = 0.968). Conclusion: FCGR3A may serve as a pivotal regulator in pediatric IBD by driving M1 macrophage polarization, representing a promising biomarker and potential therapeutic target.

The diagnostic performance of the Liver Imaging Reporting and Data System (LI-RADS) version 2018 (v2018), its modifications modified LI-RADS (mLI-RADS) and revised LI-RADS (rLI-RADS), for diagnosing hepatocellular carcinoma (HCC) remains poorly understood and requires further validation. This multicentre study aimed to evaluate the diagnostic performance of three algorithms in diagnosing HCC. We included 1092 untreated patients at risk for developing HCC who underwent gadoxetic acid-enhanced MRI across three independent cohorts from January 2020 to December 2022. Two readers independently interpreted each hepatic lesion and recorded their imaging features. The readers' judgements regarding whether the lesion was HCC or not were also noted. Non-HCC cases were confirmed based on histologic and clinical follow-up data, while HCC cases were pathologically confirmed. Diagnostic performance metrics were compared using bootstrap resampling and generalised estimating equations. Additionally, the diagnostic odds ratio (DOR) was evaluated. Of 1313 lesions, 52.3% (687/1313) were diagnosed as HCC. For all hepatic lesions, mLI-RADS achieved higher sensitivity (82.9%) and accuracy (84.1%) than LI-RADS v2018 (sensitivity, 79.9%, p = 0.024; accuracy, 83.2%) and rLI-RADS (sensitivity, 81.3%; accuracy, 83.8%), while maintaining a similar positive predictive value (mLI-RADS, 86.2%; LI-RADS v2018, 86.9%; rLI-RADS, 86.9%). The DORs were 28.3 (95% CI: 21.1-38.0) for mLI-RADS, 27.8 (95% CI: 20.6-37.7) for LI-RADS v2018 and 26.0 (95% CI: 19.3-35.0) for rLI-RADS. The readers' judgement exhibited higher accuracy than that of three algorithms (87.7%: 83.2%-84.1%). For the diagnosis of HCC in an HBV-predominant cohort, mLI-RADS showed higher performance compared with LI-RADS v2018 and rLI-RADS. Reader judgement achieved higher accuracy than all algorithms, highlighting the role of clinical expertise. NCT06663904.

Erectile dysfunction following non-nerve-sparing prostatectomy is prevalent. Although adipose-derived stem cells (ASCs) have shown therapeutic potential for erectile dysfunction, the underlying mechanisms remain unclear. To investigate the therapeutic effects of exogenous stem cells and their recruitment of endogenous stem cells in the context of postprostatectomy erectile dysfunction, we established a bilateral cavernous nerve injury (BCNI) rat model. The rats were divided into the sham surgery, BCNI + PBS, and BCNI+ASCs groups. Intracavernous pressure/mean arterial pressure ratios were compared between groups. In vivo imaging revealed ASC retention in the corpora cavernosa. Penile histomorphology and smooth muscle content were evaluated via Hematoxylin‒eosin and Masson staining. Immunohistochemistry, western blotting, and immunofluorescence were employed to quantify α-SMA, nNOS, caspase-3, vWF, Bax, Bcl-2, and SDF-1 levels and endogenous stem cell recruitment. Compared with the PBS control group, the ASC-treated group presented increased α-SMA, nNOS, and vWF expression and smooth muscle content, as well as reduced caspase-3, Bax, and Bcl-2 levels. Endogenous stem cell recruitment was observed in ASC-treated rats. Our findings demonstrate that exogenous ASCs injection suppresses apoptosis, enhances endothelial function, recruits endogenous stem cells, and alleviates erectile dysfunction in BCNI models. This study elucidates ASC-mediated mechanisms involved in erectile dysfunction recovery, providing insights for clinical translation.