HLL Lifecare (India)

Ayurvedic methods of treatment for diabetes included medicinal formulations along with suggestion of healthy diet and activities. By ayurvedic perspective treatment of diabetes depends on prognosis and pathogenesis of Prameha Vyadhi (diabetes). Vataja Prameha which can be correlated with type 2 diabetic condition is mentioned as Asadhya that is no cure but it can be manageable with Ayurveda. For this a detailed study of pathogenic process in Prameha is essential. It included etiological factors, Dosh-Dushya involved in pathogenesis, pre-symptomatic phase, symptomatic phase, complications etc. As like modern science, Ayurveda has not mentioned the specific age for prevalence of Prameha. This case is about a K/C/O type 2 DM for last 3 years to a female patient of very young age (26 years) and had just begun to show an early stage of diabetic complications like neuropathy and retinopathy. When she consulted to Jeena Sikho Lifecare Limited Hospital, Derabassi, Punjab for Ayurvedic therapy this case was diagnosed as Vataja Prameha and treatment had been started accordingly. After 7 days of Panchkarma therapies, she showed some relief in symptoms. Then she was prescribed some herbal and herbo-mineral formulations along with lifestyle recommendation. Following to 10 months of this treatment her HbA1c showed positive finding of reduction in HbA1c by 2 % as compared to before treatment finding. Her diabetic condition shifted significantly from diabetic range to non-diabetic range within 10 months. She also mentioned relief in her chief complaints present at the time of consultation. Her treatment included formulations made up of contextual anti-diabetic herbs and minerals, diet and exercise recommendation and Panchkarma therapy.

Diabetes mellitus is a lifestyle-related disease that has been recognized and described in Ayurvedic literature since ancient times. It is termed as Prameha vyadhi in Ayurveda. The characteristic symptom of the DM (Prameha) is polyurea (Bahu mutrata). It mainly causes due to Kaphaprakopak aahar-vihar. Due to this metabolic dysfunction occurs and it hampers the healthy life of a person. It affects both mental and physical health. This case study is about the male patient of age 38 years old. He came with early diagnosed type 2 diabetes to Jeena Sikho Lifecare Limited Clinic, Sangrur, Punjab on 30/11/2023. He had complaints like burning sensation in eyes, tingling numbness in legs etc. On the basis of Ayurvedic criteria he was diagnosed as Pittanubandhi Kaphaj Prameha. Ayurvedic treatment not only includes medications but also includes do’s and don’ts for diet and lifestyle (Pathya-apathya). Various drugs like Haridra, Amalaki, Shilajeet etc. are used in the above-mentioned case. This study enlightens that how ayurvedic treatment can reverse the diabetes if it is diagnosed early. Investigation reports reveal a catching reduction in HbA1c and mean blood glucose level after treatment. This ongoing case study enlightens the effectual Ayurvedic therapeutics for diabetes.

To develop and validate a simple and consistent reversed phase high performance liquid chromatography (RP-HPLC) method for the estimation of Levonorgestrel (LNG) drug from silicone based intrauterine device. Sample solution was prepared using tetrahydrofuran (THF) as solvent for the drug extraction, and RP-HPLC analysis was performed using Luna C18 analytical column (150 × 4.6mm, 5μm, 100Å - Phenomenex), with a mobile phase consisting of a mixture of acetonitrile and water (50:50, v/v) at a flow rate of 1.0ml/min and injection volume of 20μl. Detection was carried out at 241nm in PDA detector, with a total run time of 15min. The method was validated in accordance with ICH guidelines. Method applicability was tested for optimizing formulation using quality-by-design approach, to check the stability and content uniformity of levonorgestrel-silicone mixture (core blend), and quantifying the amount of LNG from commercially available silicone based formulation. The retention time for LNG drug was obtained at 8.5min (± 0.3min). A linear relationship was observed over the concentration range of 2.6-15.6μg/ml with the correlation coefficient (r) value 0.9999. The method was found to be precise within the acceptable limit (RSD < 2%) and the drug recovery from the intrauterine device was found in the range 99.78-100.0%. Content uniformity for different prototypes developed was observed in the range of 91.6-101.4%, and assay of optimized core blend was in the range of 97.78-106.79% during the 10days of retention period for stability studies. The validated method is found to be a simple, accurate, precise, reproducible, and hence can be used for the routine analysis of LNG such as in-process, quality control and stability assays of silicone based intrauterine devices by RP-HPLC.