Heinz Endowments

Several recent reports describe a role of probiotics as a therapeutic approach for celiac disease (CD). Two undigested A-gliadin peptides, P31-43 and P57-68, are central to CD pathogenesis, inducing an innate and an adaptive immune response, respectively. They enter enterocytes and localize to vesicular compartment to induce their toxic/immunogenics effects. In this article, we tested the effect of probiotic Lactobacillus paracasei (LP) CBA L74 (International Depository Accession Number LMG P-24778), its supernatant and LP-fermented cereals on gliadin peptides, P31-43 and P57-68, entrance in Caco-2 cells. Both LP CBA L74 and its supernatant inhibit P31-43 (intensity of fluorescence; FI: 75%) and P57-68 (FI: 50%) entrance in Caco2 cells, indicating that this biological effect is due to some product included in LP CBA L74 supernatant. This effect was present also after fermentation of cereals. This study describes a novel effect of probiotics in the prevention of undigested gliadin peptides toxic effects.

Background and AimsThe efficient digestion of milk triglycerides is guaranteed by the combined action of gastric lipase, colipase-dependent pancreatic lipase, and bile salt-stimulated lipase (BSSL). Human milk contains the major part of BSSL. Data regarding the dynamic changes of human milk BSSL are scarce.The aim of this study was to evaluate the changes of human milk BSSL content at different stages of lactation by proteomic techniques.MethodsWe designed a prospective longitudinal study. Human milk samples were collected from 18 healthy mothers who delivered a singleton term newborn (Gestational age 37–41 weeks) on the 3th, 7th, 15th, and 60th postpartum day. Proteomic techniques were carried out to evaluate different profile expression of BSSL in milk: in particular SDS-PAGE analysis coupled with LC-MS MS mass spectrometry.ResultsBSSL levels increased significantly from the 3th to the 60th postpartum day (figure). 357 Figure 1Human milk BSSL at different study pointsConclusionsThe increased levels of human milk BSSL could compensate for low endogenous capacity to digest dietary fat in early life as efficient absorption of lipids is important, not only for energy utilization but also for optimal growth and functional neurodevelopment.