ISCA Technologies (United States)

Spruce beetle, Dendroctonus rufipennis (Kirby) (Coleoptera: Curculionidae), is the most destructive pest of mature spruce (Picea) in western North America. Recent outbreaks in Alaska and other western US states highlight the need for tools to protect Picea from D. rufipennis. The primary antiaggregation pheromone of D. rufipennis (3-methylcyclohex-2-en-1-one, MCH) and various combinations of potential repellents (1-octen-3-ol, exo-brevicomin, endo-brevicomin, ipsdienol, ipsenol, limonene, and verbenone) were tested for their ability to disrupt the response of D. rufipennis to attractant-baited multiple-funnel traps. Two assays were conducted on the Kenai Peninsula, Alaska, in June and July 2021. All treatments significantly reduced the mean number of D. rufipennis caught compared to the baited control. No other significant differences were observed among treatments. Informed by these and other data, tree protection studies were established in Lutz spruce, Picea × lutzii, on the Kenai Peninsula in 2022 and in Engelmann spruce, Pi. engelmannii, in the Uinta Mountains, Utah, in 2021. All experimental trees were baited with frontalin. Repellent treatments included MCH (SPLAT MCH, ISCA Inc., Riverside, CA, USA) and at least 1 additional repellent combination. In Alaska, all treatments significantly reduced colonization (strip attacks + mass attacks) and mortality of individually treated Pi. × lutzii and all Picea within 11.3-m radius of each treated Pi. × lutzii compared to the control. In Utah, all treatments except for SPLAT MCH + octenol significantly reduced colonization compared to the control. Only SPLAT MCH + Acer kairomone blend (AKB) and SPLAT MCH + octenol reduced Pi. engelmannii mortality compared to the control. SPLAT MCH + AKB and SPLAT MCH + acetophenone and green leaf volatiles (PLUS) were the most effective across both studies. The implications of these and other results to the development of an effective semiochemical repellent for D. rufipennis are discussed.

The capacity of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) towreak havoc on the inflammatory and coagulation pathways via the cytokine storm has led to over 6.3 million fatalities globally. Based on recent data, the mechanism predominately involves the formation of microvascular thrombosis when pertaining to cardiovascular disease. However, a subset of coronavirus disease-2019 (COVID-19)-positive patients present emergently with acute ST-elevation myocardial infarction (STEMI) are found to have severe epicardial thrombosis which is refractory to traditional coronary revascularization. We have noted mortality in these patients presenting to our facility to be as high as 90% and all angiographically confirmed to have thrombus which was refractory to traditional therapy. We present a case series of COVID-19-positive patients presenting with STEMI found to have epicardial thrombus who were treated with different traditional STEMI therapies but with fatal outcomes. Other possible techniques including mechanical thrombectomy, optimizing traditional and nontraditional anticoagulation therapy with the use of early hemodynamic support may prove more efficacious to destroy thrombus and potentially improve mortality.

Abstract Background: Mosquitoes use odours to find energy resources, blood hosts and oviposition sites. These odour sources are normally spatio-temporally segregated in a mosquito’s life history. This study was carried out to determine the attractiveness of Anopheles gambiae to flower- and human-mimicking synthetic volatiles.Methods: This study was carried out in Muheza District in northeast of Tanzania. Anopheles gambiae s.s. Kisumu strain was used in all experiments. The chemicals/odour blends used were Vectrax, skin lure and the combination thereof. The attractiveness of Vectrax and Skin Lure were compared to each other in an insectary and semi-field settings. Semi-field experiments were then conducted using mosquito-spheres. In the two settings, parous or non-parous female mosquitoes, were simultaneously offered four treatments: Skin Lure, Vectrax, a combination of the two, and a control (no attractant). Results: In the insectary nulliparous and parous mosquitoes preferred skin odours and combinations thereof over floral odours. However, in semi-field settings significantly more nulliparous mosquitoes more caught with floral odours, whereas no differences were observed for parous females. Combining floral and human volatiles did not augment attractiveness.Conclusions: Nulliparous and parous An. gambiae s.s. are attracted to combinations of odours derived from floral and human volatiles. However, combinations of floral and skin odours did not augment attraction in semi-field settings, in spite of the fact that these blends activate distinct sets of sensory neurons. Instead, mosquito preference appeared to be modulated by blood meal experience from floral to a more generic attraction to odour blends. Results are discussed both from an odour coding, as well as from an application perspective.

Abstract Background Knowledge on mosquito attraction to various host-mimicking odors provides a potential opportunity for designing effective tools for surveillance and control of potential disease vectors. This study was carried out to explore the potential of combining plant and human mimicking odours to attract Anopheles gambiae s. s . in the laboratory and semi field settings in Tanzania. Methodology Blood fed and unfed female Anopheles gambiae s.s. mosquitoes were offered choices between human skin, plant-based odours or combination thereof in laboratory and semi-field setting. The captured mosquitoes from each source were scored for comparison. Results In laboratory and semi-field trials, the tested attractants combination of plant and human-mimicking odours found to be slightly attractive (approximately 10% higher) than either individual attractant to blood fed female mosquitoes under lab and semi-field settings. The unfed female mosquitoes were more attracted to Vectrax (plant-based materials) by more than 10% in comparison to human skin-based attractant both in lab and semi-field settings. In semi-field settings particularly, more unfed female mosquitoes were attracted to Vectrax than to the combination (p < 0.01). Conclusions The findings that the combination blend (human host and plant based) semiochemical increases mosquito attraction hold the potential to increase efficiency of mosquitoes trapping devices in both indoor and outdoor settings. This may provide valuable tools for mosquito vector management programs. Moreover, these results also suggest the potential of the combination odours to be used in the attract and kill strategies.

A Science Teachers’ Professional Development (PD) Program on Nature of Scientific Knowledge (NOSK) aspects is designed, implemented and evaluated, based on the cycle of expansive learning. A needs analysis showed that NOSK is not explicitly included in Greek Science classes and its integration might be a solution to students’ indifference towards them (questioning phase). A relevant literature review highlights three approaches to teach NOSK: through History of Science (HOS), Scientific Inquiry (SI) and Socio-scientific Issues (SSI). The PD-program includes all three, in that order, to provide the 49 participant-teachers alternative paths to embed NOSK in more school science units, designed according to the community of trainees’ Cultural-Historical characteristics and the Principles of Adult Education (analysis and modelling phases). Teachers examine and test the new model (4th phase) through a voluntary assignment to design and present a lesson plan to the plenary. The implementation phase consists of teaching in the classroom and a 5th meeting, finishing with a 6th meeting (reflecting phase). Arisen contradictions are dealt to evolve the whole activity system. Works for evaluation are included in all phases. Results to that point show that the PD-program is successful.

Na escola de Educação Infantil o fato da brincadeira acontecer em um coletivo de crianças, a torna mais rica, pois há múltiplas possibilidades para constituir e reconstituir os mais diversos vínculos entre os personagens. Cabe conhecer, nos grupos de crianças com idades diferentes, como crianças mais velhas podem afetar a brincadeira das mais novas. Observamos as brincadeiras em uma sala do Movimento Pedagógico da Escola Moderna Portuguesa (MEM) que acolhe crianças de dois a cinco anos. Para análise, tomando os papeis assumidos pelas crianças e suas ações como unidade de estudo da brincadeira, buscamos verificar se ocorrem transformações no que diz respeito à forma como as crianças mais novas interpretam os papéis. Constatamos que, na brincadeira com crianças mais velhas, as mais novas passam a assumir papéis e registramos também o aumento do número de ações na interpretação dos personagens da brincadeira. As mais novas tomam as mais velhas como modelos e passam a imitá-las o que nos remete ao conceito vigotskiano de zona de desenvolvimento próximo.

RESUMO Os Diodos Emissores de Luz, mais conhecidos como LED's, são componentes eletrônicos semicondutores que conseguem transformar a energia elétrica em luz, diferente das lâmpadas convencionais. O LED é um componente bipolar, que quando passa corrente elétrica, a luz é gerada. As lâmpadas modernas utilizam LED em sua formação, as quais liberam menos calor e utilizam menos energia, sendo assim mais econômicas que as incandescentes e as fluorescentes. Além de apresentarem maior durabilidade que as demais, as lâmpadas LED não promovem o aquecimento dos ambientes internos e seu descarte tem reduzido impacto ambiental. A associação de vantagens ambientais e econômicas torna esta tecnologia atrativa ao consumidor, embora o custo para sua aquisição seja superior aos demais modelos de lâmpadas disponíveis no mercado. Neste estudo foram realizados testes de verificação do fluxo luminoso e eficiência energética, além de análise ambiental das lâmpadas domésticas. Os resultados obtidos comprovaram o potencial da tecnologia LED em relação às demais lâmpadas analisadas, especialmente quanto à questão econômica e ambiental conforme estudos prévios analisados.

Mating disruption is a technique that uses synthetic copies of sex pheromones to control insect pests. We aimed to control Oriental fruit moth (OFM) Grapholita molesta (Busck) (Lepidoptera: Tortricidae) with formulations of SPLAT Grafo (SG) and SPLAT Grafo Attract and Kill (SGAK) in small (1 ha) apple (Malus domestica Borkh.) orchards. Our experiment was conducted in a commercial orchard with 'Gala' trees (spacing 1.5 x 4.5 m) in Vacaria, Rio Grande do Sul State, Brazil. We evaluated the effect of four treatments on G. molesta population densities: a) SG at 1 kg ha-1 (300 point sources of 3.3 g each), b) SGAK at 1 kg ha-1 (1000 point sources of 1 g each), c) insecticides as recommended by Integrated Apple Production (IAP), and d) untreated control (no treatment). Specialized Pheromone and Lure Application Technology (SPLAT) treatments were applied on 1 August 2004 and reapplied after 120 d (1 December 2004). The treatment effect was evaluated by weekly counts of males captured in Delta traps baited with commercial synthetic sex pheromone lures (eight traps per treatment). We assessed fruit damage caused by G. molesta in eight replicates of 200 fruits each on 26 October, 30 November 2004, and 5 and 31 January 2005. Applying 1 kg ha-1 of SG and SGAK in August and December 2004 significantly reduced the number of male moths caught in Delta traps. Damage to fruits at harvest, however, did not differ significantly from the control. This indicates a decline in the efficacy of mating disruption when SG and SGAK are used to protect small areas (1 ha) under high Oriental fruit moth pressure.

The ability to use inexpensive, noninvasive sensors to accurately classify flying insects would have significant implications for entomological research, and allow for the development of many useful applications in vector control for both medical and agricultural entomology. Given this, the last sixty years have seen many research efforts on this task. To date, however, none of this research has had a lasting impact. In this work, we explain this lack of progress. We attribute the stagnation on this problem to several factors, including the use of acoustic sensing devices, the over-reliance on the single feature of wingbeat frequency, and the attempts to learn complex models with relatively little data. In contrast, we show that pseudo-acoustic optical sensors can produce vastly superior data, that we can exploit additional features, both intrinsic and extrinsic to the insect's flight behavior, and that a Bayesian classification approach allows us to efficiently learn classification models that are very robust to over-fitting. We demonstrate our findings with large scale experiments that dwarf all previous works combined, as measured by the number of insects and the number of species considered.