Institute for Anthropological Research

Limonene is one of the most abundant, natural, bio-based monoterpenes. In recent years, it has attracted growing attention in both industrial and scientific communities due to its versatile physicochemical properties and wide spectrum of biological activities, including antimicrobial, antioxidant, and anti-inflammatory effects. Its renewable origin and biodegradability make limonene an ideal candidate for sustainable development and as a key building block in green chemistry. The industrial relevance of limonene spans multiple sectors, ranging from its use as a solvent and flavoring agent to its application in pharmaceuticals, cosmetics, polymers, and renewable fuels. Nevertheless, despite its numerous advantages, certain limitations and safety concerns have emerged. Prolonged or high-level exposure may result in sensitization, irritant reactions, or secondary oxidation products that pose potential health risks. Moreover, its oxidative instability can lead to the formation of reactive compounds under specific environmental conditions that influence indoor air quality and may contribute to secondary organic aerosol formation. Current research focuses on several key challenges: improving extraction and purification yields through biotechnological and enzymatic pathways; enhancing oxidative stability via encapsulation or chemical modification; and standardizing toxicological assessment protocols for both occupational and clinical settings. In this review, we analyze and discuss studies published predominantly in the last five years that explore the dual nature of limonene, its valuable industrial applications and its potential environmental and health-related challenges.

Background/Objectives: Southeastern Europe and Croatia have served as a genetic crossroads between the Near East and Europe since prehistoric times, shaped by numerous and repeated migrations. By integrating 19 newly generated ancient genomes with 285 previously published ancient genomes from Croatia, we investigated patterns of maternal and paternal landscapes from the Neolithic, Bronze, and Iron Ages through to the Antiquity and medieval periods, as well as the modern Croatian population. Methods: Ancient DNA extraction from human remains and library preparation were conducted in dedicated clean-room facilities, followed by high-throughput sequencing on the Illumina platform. Sequencing data were analyzed with established pipelines to determine mitochondrial and Y-chromosomal haplogroups and the genetic sex of individuals. Results: New ancient data reveal a predominantly European maternal profile, dominated by haplogroups H, U, and HV0, whereas Y-chromosomal lineages are characterized by J subclades and R1a, with limited representation of R1b and the absence of I2a. When combined with published ancient Croatian genomes, the results reveal similar haplogroup diversity and patterns, as well as the expansion of mtDNA haplogroup H over time and a substantial increase in Y-chromosome R1a and I2a haplogroup frequency from the prehistoric to the modern period. Conclusions: Although the analyzed samples are heterogeneous and originate from different historical periods, their genetic signatures conform to the broader patterns expected for the region. In a wider context, the ancient Croatian mitochondrial data reveal stronger genetic persistence from prehistory to modern times, unlike paternal lineages, which show significantly higher divergence.

The aim of this research was to examine the end- of-life rituals performed by nurses in hospitals in the Republic of Croatia, considering historical, social, and cultural contexts. A qualitative study was conducted using semi-structured interviews with nine nurses working in three hospitals at the secondary level of healthcare. The study explored their personal and professional practices beyond standardized proto- cols. The findings revealed diverse rituals, such as opening windows to “release the soul,” prayers, and physical care for the deceased. These practices are influenced by cultural traditions, religious beliefs, and personal values, and contribute to nurses’ emotional coping with death. The study highlights the importance of understanding cultural and personal dimensions of end-of-life care, suggesting that these practices contribute to holistic nursing and the emotional well-being of healthcare providers. Further research is needed to explore these practices across broader settings.

The study aims to reconstruct the life history of an individual whose skeleton was recovered during the excavation of the late medieval Pauline monastery of the Blessed Virgin Mary on Moslavina Mountain, Croatia. The monastery was one of the most important ecclesiastical centres in continental Croatia during the 14th/15th centuries CE and was abandoned between 1520 and 1544 due to fear of imminent Ottoman attacks. The inscription and coat of arms on the tombstone of a tomb located in the chancel, next to the main altar, indicate that the skeleton belongs to Sofia Kaštelančić née di Prata (di Pordenone), a member of Croatian late medieval high-ranking nobility. We conducted a conventional bioarchaeological study, carbon and nitrogen stable isotopes analysis, paleoradiological imaging (CT/CBCT scanning), and three-dimensional facial reconstruction. The skeleton belongs to a middle-aged woman between 40 and 50 years old with an estimated stature of about 161 cm. Numerous pathological changes, such as ante mortem tooth loss, caries, abscess, linear enamel hypoplasia, dysodontiasis, and osteophytosis were observed, with the most notable pathology being the fracture of the right ankle, a fact also confirmed by CT scanning. Carbon and nitrogen isotopic values are consistent with a terrestrial diet based on C3 plants with no marine input, and the consumption of large quantities of animal-based proteins. Three-dimensional facial reconstruction made it possible for the first time in over 500 years to obtain the approximate physical appearance of the individual. The presented results are consistent with the hypothesis that the skeleton probably belongs to Sofia Kaštelančić. Nevertheless, none of the observed osteological traits are individually or collectively diagnostic of Sofia, so, in the absence of individualising evidence, the identification remains hypothetical rather than demonstrative.

By 2045, approximately 783.2 million people are projected to be diagnosed with type 2 diabetes mellitus (T2DM). In addition, obesity is expected to affect up to 22% of the world’s population or one in four people. The diabesity epidemic, a worrying trend in which T2DM and obesity co-occur, is becoming increasingly evident and could be the most significant epidemic of non-communicable chronic diseases in human history. The salutogenic pattern of health, which emphasises well-being and resistance resources, could be a promising solution to address this alarming worldwide problem. The salutogenic pattern of health has numerous positive effects on the health of persons with T2DM. These include reducing the risk of it, lowering some biomarkers and laboratory parameters related to its control, and promoting a better lifestyle, ultimately improving the overall quality of life. The salutogenic pattern of health offers an effective and evidence-based approach to address the growing global problem of chronic non-communicable diseases such as T2DM. Integrating this theory into standard modern medical practice has the potential to significantly improve health outcomes and overall patient well-being, making it an important direction for modern medicine. Accordingly, the aim is to explore and analyse the salutogenic pattern of health associated with T2DM in order to prevent it, but also the better management of it.

An integrated framework is introduced and applied to assess the health impact of airborne pollution with greater physiological relevance, moving beyond conventional exposure metrics. Measured particle number size distribution data was integrated with a regional respiratory tract deposition fractions to estimate total and alveolar deposited particle surface area concentrations. Land use regression modeling, combined with randomized commuting patterns, enabled the evaluation of city-specific alveolar surface area deposition doses, providing new insight into localized average exposure and its implications for public health. The results showed that although the mean street-level air pollution in Lithuania is higher than in other European cities, the urban background levels are on the same level. We found that the total respiratory deposited surface area concentration is up to 18-fold higher due to coarse particles, which also determines alveolar deposited particle surface area dose. Our findings advocate for using integrated pollution assessments and region-specific policies rather than broad diesel vehicle-targeted bans. The proposed methodology is expected to enhance traditional exposure assessments by switching to lung deposited surface area, which can be further refined by incorporating daytime activity patterns, socio-economic status, and personal health conditions.

Loteprednol etabonate (LE) is a soft corticosteroid recently approved for the short-term treatment of signs and symptoms of dry eye disease. LE is available on the market as a suspension, which can release only limited amount of dissolved corticosteroid after application at the ocular surface. This study is focused on the development of an oil-in-water nanoemulsion (NE) to effectively deliver dissolved LE to the ocular surface, in order to promote its absorption. We newly developed an extended-throughput 3D model of the corneal epithelium for biocompatibility study, and an innovative approach to investigate the effect of biorelevant dilution on LE release from the NE oil phase. Castor oil, Capryol® 90, Kolliphor® EL and Soluplus® were selected as NE excipients based on their potential to dissolve LE. Design of experiments was successfully employed to develop biocompatible and physically stable NE with high LE content (0.15 %, w/w) and retention efficiency (87 % after 10 months of storage at room temperature). LE retention within oil droplets (above 90 % of the initial LE content) despite biorelevant dilution simulating tear turnover suggests the potential for direct LE absorption from the NE oil droplets into the lipophilic corneal epithelium. The results obtained encourage the extension of the studies in terms of in vitro permeability and in vivo eye-related bioavailability assessment to prove the potential of the proposed LE formulation.