Istituto De Angeli (Italy)

ABSTRACTExtracellular vesicles (EVs) have emerged as powerful mediators of intercellular communication with significant potential across biomedical, veterinary, cosmetic, agricultural and environmental applications. However, the translation of EV‐based discoveries into practical and commercially viable products remains constrained by scientific complexity, regulatory uncertainty and manufacturing challenges. To address these barriers, the International Society for Extracellular Vesicles (ISEV) established the Translation, Regulation and Advocacy Committee (ISEV‐TRA). ISEV‐TRA aims to catalyse the responsible advancement of EV technologies by fostering cross‐sector collaboration, harmonising quality and regulatory frameworks and providing strategic advocacy to enhance market readiness. Through targeted initiatives—such as workshops and the development of translational resources and guidance—ISEV‐TRA seeks to bridge the gap between research and real‐world implementation. By promoting dialogue amongst academia, industry, investors and policymakers, ISEV‐TRA positions itself as a central driver in shaping the global roadmap for EV translation and commercialisation.

La presente investigación abordó la planificación financiera y la rentabilidad en una empresa de formación tecnológica y técnica durante el 2024, vinculándose al Objetivo de Desarrollo Sostenible (ODS) N.º 8: “Trabajo decente y crecimiento económico”, especialmente a las metas 8.1 y 8.3, que promueven el crecimiento económico sostenido y la formalización empresarial. El estudio fue de tipo básico, con enfoque cuantitativo y diseño no experimental, descriptivo y de corte transversal. La muestra estuvo conformada por 15 trabajadores de las áreas de gerencia, contabilidad y administración. Se aplicó directamente un cuestionario estructurado a los participantes para recolectar los datos necesarios. Los resultados mostraron que el 100% de los encuestados calificaron la planificación financiera como regular, debido a deficiencias en el seguimiento presupuestario y la falta de coordinación entre áreas. En cuanto a la rentabilidad, el 87.0% la calificó como regular, mientras que el 13.0% la consideró óptima. Se concluyó que la empresa presentó limitaciones en su gestión financiera, ya que los ingresos apenas cubrieron los gastos operativos y existió poca diversificación de fuentes de ingreso, lo que afectó negativamente su rentabilidad y sostenibilidad.

Rett syndrome (RTT) is a severe neurodevelopmental disorder primarily affecting females, with an incidence of 1 in 10,000 live births. It is caused mainly by de novo mutations in the X-linked MECP2 gene, which encodes methyl-CpG binding protein 2 (Mecp2), a key epigenetic regulator. MECP2 mutations have profound impacts on neurons, which exhibit morphological, synaptic and functional impairments. However, more recent evidence highlights a crucial role of astrocytes in RTT pathogenesis. Indeed, RTT astrocytes exhibit structural and functional impairments, failing to support neuronal growth and function through non-cell autonomous mechanisms. Studies reveal that MECP2 deficient astrocytes secrete abnormal factors that impair neuronal growth and synaptic function. Furthermore, they show dysregulated calcium signalling, disrupted glutamate and potassium homeostasis, and increased inflammatory responses, all of which contribute to neuronal dysfunction. Understanding these neuron-astrocyte interactions may offer novel therapeutic targets for RTT. In the review we aim at presenting the current knowledge of astrocyte-neuron crosstalk in RTT, describing the different mechanisms highlighted so far through which MECP2 mutant astrocytes impair neurons. Finally, we discuss existing and prospective methodological approaches for investigating cell-to-cell communication in RTT.

Abstract Microfluidic platforms for clinical use are a promising translational strategy for cancer research specially for drug screening. Identifyng Cancer Stem Cells (CSC) using sphere culture techniques in microfluidic devices (MDs) showed to be better reproducing physiological responses than other in vitro models and allow the optimization of samples and reagents. We evaluated individual spheres proliferation and stemness towards chemotherapeutic treatment (CT) with doxorubicin and cisplatin in bladder cancer cell lines (MB49-I and J82) cultured in MDs used as CSC treatment response platform. Our results confirm the usefulness of this device to evaluate the CT effect in sphere-forming efficiency, size and growth rate from individual spheres within MDs and robust information comparable to conventional culture plates was obtained. The expression of pluripotency genetic markers (Oct4, Sox2, Nanog and CD44) could be analyzed by qPCR and immunofluorescence in spheres growing directly in MDs. MDs are a suitable platform for sphere isolation from tumor samples and can provide information about CT response. Microfluidic-based CSC studies could provide information about treatment response of cancer patients from small samples and can be a promising tool for CSC-targeted specific treatment with potential in precision medicine.

The aim of this study was to estimate the contribution of adult attachment style and alexithymia to cutaneous body image in patients with psoriasis while controlling for the confounding effects of disease severity and depressive symptoms. Participants were 107 consecutive patients (60% women) with a diagnosis of plaque-type psoriasis. Cutaneous body dissatisfaction was measured with the Cutaneous Body Image Scale (CBIS) and disease severity with the Psoriasis Area and Severity Index (PASI). Depressive symptoms, attachment style and alexithymia were assessed with the Beck Depression Inventory (BDI), the Attachment Style Questionnaire (ASQ) and the Toronto Alexithymia Scale (TAS-20), respectively. Hierarchical regression analysis showed that, independently from the severity of skin disease and depressive symptoms, patients with an insecure-avoidant attachment style and higher levels of alexithymia reported greater cutaneous body dissatisfaction. Because of the cross-sectional design, the causal relationship between avoidant attachment, alexithymia and cutaneous body image cannot be determined. There was no control group. Clinical assessment of patients with psoriasis should include attachment style and alexithymia among the psychological variables related to cutaneous body dissatisfaction.

L’articolo analizza La Difesa delle Lavoratrici, primo periodico nazionale delle donne socialiste fondato da Anna Kuliscioff, pubblicato in Italia dal 1912 al 1925. Sul periodico scrivono e discutono le principali esponenti del socialismo dell’epoca, iscritte al Psi; troviamo però anche testimonianze di donne lavoratrici che scrivono lettere al giornale parlando dei problemi della vita quotidiana. Le donne che scrivono nel giornale svolgono un compito fondamentale di propaganda fra donne quasi analfabete, e possono essere considerate le progenitrici della politica nel primo partito di massa. Di particolare interesse, le discussioni fra interventiste e pacifiste e le polemiche sull’autonomia delle sezioni femminili rispetto a quelle maschili.