Jingning County People's Hospital

BackgroundFor patients with poor glycemic control of adult type 1 diabetes mellitus (T1DM), in addition to managing blood glucose levels, it is worth exploring further as an early warning indicator of blood glucose control.Methods56 adults with T1DM, aged between 18 and 70 years, were included in the study. Data on body composition and laboratory indicators, including phase angle values and HbA1c levels, were collected. Statistical analysis was conducted to determine the correlation and the strength of association between the phase angle, and HbA1c levels.ResultsPearson’s correlation and linear regression models indicated a negative correlation between phase angle and HbA1c levels, even after controlling for age and weight in both males and females.ConclusionThe results indicate a significant negative relationship between Phase angle and HbA1c levels. Beyond being a simple body composition parameter, Phase angle can be used as a clinical indicator of improved blood glucose control.

Cheirotonus jansoni is a nationally protected and ornamental beetle species endemic to southern China, northern Burma, and Vietnam. Due to habitat degradation and illegal collection, its populations have declined significantly, prompting urgent conservation efforts. Here, we present a high-quality, chromosome-level genome assembly for C. jansoni to support future ecological and evolutionary studies. Male adults were collected from Qingliangfeng national nature reserve, and high molecular weight DNA was extracted for sequencing. The genome was assembled using PacBio long reads and scaffolded with Hi-C chromatin conformation data. The final assembly spans approximately 620.03 Mb, with a scaffold N50 of 101.60 Mb and 98.9% of sequences anchored to 10 chromosomes. Benchmarking with BUSCO revealed a 93.6% completeness rate, indicating high assembly quality. This genomic resource provides a valuable foundation for studying the genetic basis of adaptation, population decline, and conservation planning in C. jansoni and related species.

This study aimed to report the genome of carbapenem-resistant ST58 Escherichia coli strain EC6622, isolated from the urine sample of a patient with lower limb fracture, and to explore the genomic characteristics of the bla NDM-5-harboring IncHI2/IncHI2A plasmid and the multi-resistance IncF plasmid carried by strain EC6622. The genome of E. coli strain EC6622 was fully sequenced using the Illumina and PacBio platforms, followed by hybrid assembly of the genome sequences. The identification of antibiotic resistance genes (ARGs) and the genetic context of ARGs were accomplished using bioinformatics tools. Comparative genomic analyses of the resistance plasmids carried by E. coli strain EC6622 were performed. E. coli strain EC6622, belonging to ST58 (Achman scheme) or ST87 (Pasteur scheme), harbored bla NDM-5 on IncHI2/IncHI2A plasmid pEC6622-1. bla NDM-5 was embedded in ΔISKox3-IS3000-ΔISAba125-IS5-bla NDM-5-ble MBL-trpF-dsbC-ΔcutA-IS26-ΔISVsa5-IS3000-IS1A. In addition, two class 1 integrons were also found in the IncHI2/IncHI2A plasmid pEC6622-1. IncFIBAP001918/IncFII plasmid pEC6622-2 carried one 23-kb multidrug-resistant (MDR) region, including tet(A)-tetR(A), class 1 integron, and a Tn3-like region containing mer operon and bla TEM-1B gene, which were nearly identical to those of seven IncF plasmids distributed in E. coli strains. In the carbapenem-resistant E. coli strain EC6622 isolated from the urine sample of a 70-year-old patient with a lower limb fracture, the carbapenemase gene bla NDM-5 was carried by the IncHI2/IncHI2A plasmid (pEC6622-1), while a coexisting IncFIBAP001918/IncFII hybrid multidrug-resistance plasmid (pEC6622-2) was also identified. These findings provide detailed genetic information that enhances our understanding of the resistance mechanisms and potential dissemination routes of bla NDM-harboring E. coli.

ABSTRACTRapid eye movement (REM) sleep, also known as paradoxical sleep (PS), was first identified in humans and cats in the late 1950s. It is hypothesized to contribute to brain development by providing endogenous neural activity patterns that coincide with critical periods of maturation. We summarize the remarkable alterations in polysomnographic data, EEG power spectral analysis, and behavioural patterns during early‐life REM sleep, as well as the subsequent maturation of these features and the development of regulatory mechanisms. REM sleep disturbances in early life are linked to neurodevelopmental disorders. Our review aims to elucidate changes in the main characteristics during REM sleep development and its evolutionary connection with brain development, ultimately providing a basis for promoting the prevention and treatment of various neurodevelopmental disorders.

To explore the expression of matrix metalloproteinase 9 and miRNA-337-3p in oral squamous cell carcinoma tissues and analyse their clinical significance. Postoperative specimens from 30 oral squamous cell carcinoma (OSCC) patients were collected from June 2022 to January 2023, and real-time fluorescence quantitative reverse transcription PCR was used to detect the expression levels of miR-337-3p in cancer tissues and adjacent normal tissues. The correlation between the expression levels of the two and the clinicopathological parameters of patients was analysed. Compared with adjacent normal tissues, the expression of miRNA-337-3p in OSCC tissues was significantly lower (p < 0.05), and its expression level was clearly related to the lymph node metastasis status of OSCC patients (p < 0.05). The positive rates of MMP-9 in OSCC tissue and adjacent normal tissue were 83.33% and 36.67% respectively. Compared with patients in the N0 stage, the expression level of miRNA-337-3p in OSCC tissues of patients in the N1 + N2 stage was significantly reduced (p < 0.05). The expression of MMP-9 and miRNA-337-3p in OSCC tissues is related to cervical lymph node metastasis. miRNA-337-3p plays a tumour suppressor role in OSCC and may inhibit OSCC cell invasion and metastasis.

Music therapy is increasingly acknowledged as a non-pharmacological approach that fosters emotional, cognitive, and social development in individuals with special needs. However, existing research often faces limitations such as small sample sizes, short program durations, and limited participant diversity, which restrict the generalization of findings. To address these challenges, the present research evaluates the effectiveness of a structured ten-week music therapy program aimed at improving the overall quality of life in three participant groups: individuals with physical disabilities, cognitive impairments, and psychological disorders. The primary objective of this research is to determine how music therapy influences emotional well-being, cognitive stimulation, social interaction, and self-perception. A total of seventy-five participants (n = 75) were recruited from rehabilitation centers, community organizations, and mental health facilities The intervention included rhythmic exercises, singing, instrumental play, and guided improvisation. Data were collected through a five-point Likert-scale questionnaire and facilitator observations conducted before and after the program. Statistical analyses were performed using the SPSS, Version 29.0. Paired-sample t-tests and repeated measures analysis of variance were used to examine both within group and between-group differences. The results revealed significant improvements in emotional well-being (p &lt; 0.05) and social engagement (p &lt; 0.01), with moderate increases in cognitive stimulation. Participants reported a fifteen to twenty-five percent increase in life satisfaction, demonstrating the holistic effect of music therapy. In conclusion, the study confirms that structured music therapy programs are effective, inclusive, and evidence-based interventions that promote emotional expression, social connection, and overall life satisfaction among individuals with special needs.

Huntington's disease (HD) is an autosomal dominant neurodegenerative disorder caused by CAG repeat expansion in the HTT gene. Existing toxin-induced and genetic models provide important insights, but none fully replicate the progressive pathology of HD. An AAV9-mediated striatal mouse model expressing mutant HTT with 82 CAG repeats was established to reproduce hallmark neuropathological changes and behavioral deficits. Male C57BL/6 mice received bilateral intrastriatal injections of AAV9-HTT-82Q or control AAV9-GFP. Behavioral performance was assessed by rotarod, balance beam, open field, and Y-maze tests. Neuropathology was examined with HE/Nissl staining, TUNEL assay, and immunofluorescence for mHTT, DARPP-32, GFAP, and Iba1. AAV9-82Q mice exhibited progressive motor coordination deficits on the rotarod from Week 4 and impaired beam traversal from Week 18. Open field testing revealed persistent hyperactivity from Week 8, while anxiety-like and cognitive measures showed only mild, non-significant trends. Histological analysis demonstrated extensive mHTT aggregation in the striatum, accompanied by neuronal pyknosis, vacuolization, and significant loss of Nissl-positive neurons. TUNEL staining confirmed increased apoptosis. Immunofluorescence further revealed selective reduction of DARPP-32+ medium spiny neurons, along with marked astrogliosis and microgliosis, indicating robust neurodegeneration and inflammatory responses. The AAV9-82Q model induces adult-onset, progressive HD-like pathology with early motor impairments, neuronal loss, and glial activation. It complements existing models and provides a reproducible platform for mechanistic studies and preclinical therapeutic evaluation.

Nonpharmacological therapies like acupuncture, gua sha, and cupping are gain-ing prominence in integrative medicine, yet their application lacks data-driven personalization and rigorous safety assurances. This paper introduces Meridi-anFM, a causal and safe multimodal foundation model framework designed to generate personalized prescriptions for these therapies. MeridianFM integrates four key innovations: (1) a meridian-aware graph neural network that encodes the topological and semantic properties of acupoints; (2) a self-supervised mul-timodal architecture that fuses physiological time series, thermal imagery, elec-tronic health records, and patient-reported outcomes; (3) a causal inference layer employing doubly robust estimation and front-door adjustment to miti-gate confounding in observational data and estimate individualized treatment effects; and (4) a constrained reinforcement learning policy optimized with risk-sensitive objectives (Conditional Value-at-Risk) and a mixed-integer pro-gramming post-processor to enforce clinical safety and feasibility constraints. To ensure reproducibility, we provide a comprehensive evaluation suite based on synthetic and semi-synthetic data, including all source code. Our experi-ments demonstrate that MeridianFM surpasses baseline models in optimizing treatment policies, accurately estimating causal effects, and adhering to safety constraints. While this study establishes methodological feasibility and superior performance in simulated environments, it also lays the groundwork for future clinical validation, representing a significant step toward AI-driven precision in traditional medicine.

Curcumin is a natural polyphenolic compound extracted from the rhizomes of Curcuma longa, exhibiting a wide range of biological activities, including anti-inflammatory, antioxidant, antitumor, antibacterial, antiviral, and neuroprotective effects. However, its low oral absorption rate and poor bioavailability limit its clinical application. To address this issue, this study synthesized a novel curcumin derivative, AN02, which significantly improves the absorption rate and bioavailability while enhancing its antitumor activity. This study focused on the antitumor mechanism of AN02 in ovarian cancer, particularly its ability to inhibit ovarian cancer cell proliferation, invasion, and migration by regulating the APC (Adenomatous Polyposis Coli)-SMAD4 (SMAD family member 4)-CTLA-4 (Cytotoxic T-Lymphocyte-Associated Protein 4) molecular axis. Experimental results demonstrated that AN02 significantly inhibited ovarian cancer cell proliferation at very low concentrations, with its half-maximal inhibitory concentration (IC50) significantly lower than that of curcumin. Additionally, AN02 exerted its antitumor effects by activating the APC-SMAD4 molecular axis and inhibiting the CTLA-4 molecular axis. Silencing CTLA-4 inhibits the proliferation and immune escape of ovarian cancer. Further molecular mechanism studies revealed that APC directly regulates the SMAD4-CTLA-4 molecular axis, while SMAD4 forms a chaperone relationship with CTLA-4 and promotes CTLA-4 degradation through the K48-dependent ubiquitination pathway, thereby suppressing the malignant phenotype of ovarian cancer cells. These findings not only reveal the antitumor mechanism of AN02 but also provide new insights for the treatment of ovarian cancer. Animal experiments also demonstrated that AN02 significantly inhibits the proliferation of subcutaneous xenograft tumors in mice. As a novel curcumin derivative, AN02 exhibits significant antitumor activity and inhibits ovarian cancer progression by regulating the APC-SMAD4-CTLA-4 molecular axis. This study lays an important theoretical foundation for the development of novel antitumor drugs based on AN02, with significant clinical application potential. However, the long-term toxicity and safety of AN02 require further investigation to establish safe dosage standards for clinical use. Future studies will focus on exploring combination therapy strategies of AN02 in cisplatin-resistant ovarian cancer to provide new directions for precision treatment of ovarian cancer.