Kacho College

目的 前報と同じく, 多面的感情状態測定尺度を用いて, 女子学生を対象に, 着装によって生起する感情状態を測定した.本報では, 「卒業記念パーティ」, 「通学」, 「レジャーランドへの小旅行」, 「会社訪問」の各場面において, 「ふさわしい/ふさはしくない」あるいは「着たい/着たくない」と評価した衣服を着用したときに生起する「快活・爽快」, 「充実」, 「優越」, 「安らぎ」および「抑欝・動揺」, 「董恥」, 「圧迫・緊張」の各感情を測定し, 衣服の評価とその衣服を着たときに喚起する感情との関係を求めることを目的としている.成果 「ふさわしい/ふさわしくない」と「着たい/着たくない」の2つの尺度 (それぞれ5水準) によって評価された衣服を着装したときに生起する感情の状態を比較すると, 2つの尺度ともその評価に対応して7感情が生起していることがわかった.場面にふさわしい, 着たいと評価された衣服を着用した場合に, 「快活・爽快」の感情は各場面で強く生起し, 特にレジャーランドへの小旅行, 通学の場面でより強く生起している.「優越」の感情は卒業パーティで, 「充実」の感情は会社訪問, 卒業パーティで, 「安らぎ」の感情は小旅行, 通学の場面で強く生起している.一方, 場面にふさわしくない, 着たくないと評価された衣服を着用した場合には, 各場面とも「羞恥」の感情が特に強く生起し, 「抑欝・動揺」の感情が次いで強く生起している.「圧迫・緊張」の感情はふさわしい, 着たいと評価された衣服を着用した場合においても, 場面によって感情の生起に大きな違いがあることがわかった.

A novel endo-β-N-acetylglucosaminidase acting on complex type sugar chains of glycoproteins was found in the culture broth of a fungus isolated from soil and identified as Mucor hiemalis f. hiemalis on the basis of various characteristics. The endo-β-N-acetylglucosaminidase, named Endo-M, was purified to almost homogeneity by polyacrylamide gel electrophoresis involving ammonium sulfate fractionation, and column Chromatographies on DEAE-Sepharose CL-6B, Sephadex G-200, hydroxylapatite, TSK-gel HW-65F and Con A-Sepharose 4B. The molecular weight of the enzyme was estimated to be about 95,000 by gel chromatography. The optimum pH was found to be 6.0–6.5 and the enzyme was stable in the pH range of 7 to 8. The enzyme showed high activity on dansyl ovalbumin glycopeptide, and also could act on dansyl transferrin glycopeptide and dansyl asialotransferrin glycopeptide containing biantennary complex type sugar chains. The Km value for dansyl asialotransferrin glycopeptide as the substrate was 2.0 × 10−3 m. The enzyme released complex type sugar chains from intact asialotransferrin without the addition of any detergent and the liberated sugar chains were identified by chromatography on a Bio-Gel P-4 column, calibrated with markers of known structure, and 1H-NMR analysis.

Abstract To improve the low water wettability of poly(ethylene terephthalate) (PET), graft polymerization of acrylamide (AAm) by UV irradiation was performed onto the surface of a PET film with the simultaneous irradiation method without using a photo sensitizer. The PET film immersed in a 10 wt % deaerated aqueous solution of AAm was found to become highly hydrophilic upon UV irradiation. Optical microscopy on cross sections of grafted films showed that localization of the graft polymerization was restricted to a thin surface region of the film. Both the low concentration of polymer radicals formed by UV irradiation and the monomer penetration limited to the film surface would be responsible for localization of the grafted layer to the film surface region. Pretreatment of the PET film with benzyl alcohol was effective for enhancement of the graft polymerization. Retention of high hydrophilicity of the surface even after rigorous extraction of homopolymer and a comparative study of polymerization without UV irradiation strongly suggested that UV irradiation of the PET film under immersion in the deaerated AAm aqueous solution would lead to formation of the true graft copolymer.