Landesbetrieb Landwirtschaft Hessen

Abstract Central Europe experienced severe droughts from 2018 to 2020, which led to bark beetle infestations in Norway spruce (Picea abies L.). While recent studies have identified these droughts as a major factor in the dieback of spruces, the exact mechanism through which drought stress influences spruce vitality and increases vulnerability to infestations is not well understood. To gain insights into the spatial and temporal relations between infestations and drought stress, this study compared soil moisture index (SMI) data for the entire soil layer from the German Drought Monitor and Sentinel-2 satellite time series for healthy and bark beetle infested spruce stands preceding the infestation. The study used t-tests to compare pre-infestation monthly sums of SMI and vegetation indices (VIs) for healthy and infested stands. Yearly trajectories of six VIs were used to compare the separability between infested and healthy plots. The results showed that the monthly sum values of SMI for healthy spruce plots were statistically significantly higher than the infested plots from May 2017 to January 2018, while the monthly sum values of Inverted Red Edge Chlorophyll Index of healthy spruce plots were significantly higher than infested plots during growing seasons. With these findings, the study provides useful information for the understanding of the relation of soil moisture and remote sensing VIs patterns in relation to bark beetle infestations.

Wstp

Summary Scientific facts about the relationships between the goals are indispensable for the rational social discourse on solving the various conflicting goals on the way to sustainable crop production. Therefore, the German Society for Agronomy has compiled scientific findings on the topics of biodiversity, climate relevance and climate resilience, nutrient management, crop protection, soil protection and production of renewable raw materials, and identified the existing need for research. After an executive summary that highlights the findings, the subject areas are explained in detail. The purpose of this paper is not to make recommendations for policy-making, but to support the public debate by providing factual information.

Wildlife animals may be susceptible to multiple infectious agents of public health or veterinary relevance, thereby potentially forming a reservoir that bears the constant risk of re‐introduction into the human or livestock population. Here, we serologically investigated 493 wild ruminant samples collected in the 2021/2022 hunting season in Germany for the presence of antibodies against the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) and four viruses pathogenic to domestic ruminants, namely, the orthobunyavirus Schmallenberg virus (SBV), the reovirus bluetongue virus (BTV) and ruminant pestiviruses like bovine viral diarrhoea virus or border disease virus. The animal species comprised fallow deer, red deer, roe deer, mouflon and wisent. For coronavirus serology, additional 307 fallow, roe and red deer samples collected between 2017 and 2020 at three military training areas were included. While antibodies against SBV could be detected in about 13.6% of the samples collected in 2021/2022, only one fallow deer of unknown age tested positive for anti‐BTV antibodies, and all samples reacted negative for antibodies against ruminant pestiviruses. In an ELISA based on the receptor‐binding domain (RBD) of SARS‐CoV‐2, 25 out of 493 (5.1%) samples collected in autumn and winter 2021/2022 scored positive. This sero‐reactivity could not be confirmed by the highly specific virus neutralisation test, occurred also in 2017, 2018 and 2019, that is, prior to the human SARS‐CoV‐2 pandemic, and was likewise observed against the RBD of the related SARS‐CoV‐1. Therefore, the SARS‐CoV‐2 sero‐reactivity was most likely induced by another hitherto unknown deer virus belonging to the subgenus Sarbecovirus of betacoronaviruses.

LebensmittelchemieVolume 76, Issue S1 p. S1-090-S1-090 Article Bestimmung der Fehlaromen von nativen Olivenölen mittels SPME-GCxGC/MS Dr. M. Kresse, Corresponding Author Dr. M. Kresse Michael.kresse@landeslabor-bbb.de Berlin/D Landeslabor Berlin-Brandenburg, Fachbereich I-2, Rudower Chaussee 39, 12489 BerlinMichael.kresse@landeslabor-bbb.deSearch for more papers by this authorM. Kaufmann, M. Kaufmann Berlin/DSearch for more papers by this authorTh. Biederbick, Th. Biederbick Berlin/DSearch for more papers by this author Dr. M. Kresse, Corresponding Author Dr. M. Kresse Michael.kresse@landeslabor-bbb.de Berlin/D Landeslabor Berlin-Brandenburg, Fachbereich I-2, Rudower Chaussee 39, 12489 BerlinMichael.kresse@landeslabor-bbb.deSearch for more papers by this authorM. Kaufmann, M. Kaufmann Berlin/DSearch for more papers by this authorTh. Biederbick, Th. Biederbick Berlin/DSearch for more papers by this author First published: 01 March 2022 https://doi.org/10.1002/lemi.202255003AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat No abstract is available for this article. Volume76, IssueS1Supplement: Abstracts der Vorträge der Regionalverbände und die der Posterflashtalks der AG JLCMarch 2022Pages S1-090-S1-090 RelatedInformation

Background: Whole-genome sequencing has become routine for population genetic studies. Sequencing of individuals provides maximal data but is rather expensive and fewer samples can be studied. In contrast, sequencing a pool of samples (pool-seq) can provide sufficient data, while presenting less of an economic challenge. Few studies have compared the two approaches to infer population genetic structure and diversity in real datasets. Here, we apply individual sequencing (ind-seq) and pool-seq to the study of Western honey bees (Apis mellifera). Methods: We collected honey bee workers that belonged to 14 populations, including 13 subspecies, totaling 1347 colonies, who were individually (139 individuals) and pool-sequenced (14 pools). We compared allele frequencies, genetic diversity estimates, and population structure as inferred by the two approaches. Results: Pool-seq and ind-seq revealed near identical population structure and genetic diversities, albeit at different costs. While pool-seq provides genome-wide polymorphism data at considerably lower costs, ind-seq can provide additional information, including the identification of population substructures, hybridization, or individual outliers. Conclusions: If costs are not the limiting factor, we recommend using ind-seq, as population genetic structure can be inferred similarly well, with the advantage gained from individual genetic information. Not least, it also significantly reduces the effort required for the collection of numerous samples and their further processing in the laboratory.

Matrix-assisted laser-desorption/ionization-time-of-flight-mass-spectrometry (MALDI-TOF-MS) is widely used to identify microorganisms. Recently, new applications such as identification of the animal species from meat, milk or fish are emerging. Standards for the validation of species identifications are still missing. Now, the § 64-LFGB working-group “MALDI-TOF”, established at the Federal Office of Consumer Protection and Food Safety, has compiled a guideline for the validation of species identifications. This guideline is intended for single laboratories as well as for lab networks and shows practical ways for validation of qualitative MALDI-TOF-MS methods. The special opportunities of the technology, in particular the use of extended reference databases and of collections of well-documented individual spectra for validation, have been taken into account in the guideline presented.

Simple SummaryThis study is the first to compare the associative learning performance of six honeybee subspecies from different European regions in a common apiary. We quantified sucrose responsiveness prior to appetitive olfactory proboscis extension learning to dissociate effects of motivation and cognition. Our results show that Apis mellifera iberiensis displayed a significantly poorer learning performance compared to other Apis subspecies from across Europe, which did not differ from each other. Possible causes are discussed.The Western honeybee (Apis mellifera L.) is one of the most widespread insects with numerous subspecies in its native range. How far adaptation to local habitats has affected the cognitive skills of the different subspecies is an intriguing question that we investigate in this study. Naturally mated queens of the following five subspecies from different parts of Europe were transferred to Southern Germany: A. m. iberiensis from Portugal, A. m. mellifera from Belgium, A. m. macedonica from Greece, A. m. ligustica from Italy, and A. m. ruttneri from Malta. We also included the local subspecies A. m. carnica in our study. New colonies were built up in a common apiary where the respective queens were introduced. Worker offspring from the different subspecies were compared in classical olfactory learning performance using the proboscis extension response. Prior to conditioning, we measured individual sucrose responsiveness to investigate whether possible differences in learning performances were due to differential responsiveness to the sugar water reward. Most subspecies did not differ in their appetitive learning performance. However, foragers of the Iberian honeybee, A. m. iberiensis, performed significantly more poorly, despite having a similar sucrose responsiveness. We discuss possible causes for the poor performance of the Iberian honeybees, which may have been shaped by adaptation to the local habitat.

Abstract In organic farming the control of perennial weed species, in particular Cirsium arvense, can be a major concern for farmers, especially if there is no regulation through perennial forage production. To test whether the stubble cleaner (SC), an enhanced skim plow (PL), is as effective in the control of C. arvense and other weeds as conventional ploughing and perennial forage production, an organic field experiment was established. Three different tillage/crop rotation systems were compared: an SC system and a PL system, both in a cereal-based crop rotation and an additional PL system in a crop rotation that included a perennial alfalfa–grass mixture (PLALF). In the SC system, tillage was carried out solely with the SC, while in the PL and PLALF systems, ploughing was alternated with chiseling. In the fifth year, each main plot was divided into subplots, and seven different cover crop treatments were integrated into each of the three systems. The effects of the three systems and the cover crop treatments on weed cover and density, weed biomass, and weed diversity in the sixth and seventh year of the experiment are the subjects of this paper. The choice of cover crop species was of minor importance for weed control. The PLALF system was generally more effective in controlling C. arvense than the PL and SC systems. No significant differences between the PL and SC systems regarding the control of C. arvense could be identified in four of five assessments. The SC system had significantly higher total weed density than the PLALF and PL systems in both years. However, the differences in weed emergence between the PL and SC systems diminished until the assessment of weed cover and biomass in the main crops, when no significant differences between these two systems (2012) or no differences at all (2013) could be identified. Species richness was not significantly influenced by the tillage/crop rotation system in both years. Evenness and Shannon–Wiener index were significantly higher in the PLALF and PL systems than in the SC system on most assessment dates in 2012. In 2013 there was no clear trend regarding evenness and Shannon-Wiener index probably due to a hoeing operation.. In conclusion, for weed control, the choice of crop rotation was more important than the choice of tillage method, while for the diversity of the weed community, the choice of tillage method was more important than the crop rotation.