Landesuntersuchungsamt Rheinland-Pfalz

Information on the molecular epidemiology and carbapenem resistance mechanisms in Gram-negative bacterial isolates in Moldova is scarce. To close this knowledge gap, carbapenem-resistant Gram-negative bacteria were collected over an 11-month period in a routine diagnostic laboratory in Moldova. Antimicrobial susceptibility was phenotypically and genotypically assessed. Phylogenetic relationships were investigated and multi-locus sequence types were provided. The assessment indicated several clusters of phylogenetically closely related carbapenem-resistant Klebsiella pneumoniae (sequence types ST101, ST395 and ST377), Acinetobacter baumannii (ST2, ST19 and ST78) and Pseudomonas aeruginosa (ST357 and ST654) isolates next to a number of less frequently observed species and sequence types. A phylogenetic relationship to characterized isolates from neighboring Ukraine could be confirmed. Identified carbapenemase genes comprised blaOXA-23, blaOXA-72 and blaGES-11 in A. baumannii, blaKPC-3, blaNDM-1 and blaOXA-48 in K. pneumoniae, as well as blaVIM-2 in Pseudomonas aeruginosa. In conclusion, the assessment suggested the spread of carbapenem-resistant Gram-negative bacteria in Moldova which were partly pre-described from neighboring Ukraine, as well as likely spill-over events, facilitating the regional spread of carbapenem-resistant clones. Several isolates with very high genomic similarity further support the hypothesis of likely regional transmission events driven by several evolutionary successful clonal lineages.

Ines Mayer/Reinhold Weber (Hg.): Menschen, die uns bewegten. 20 deutsche Biographien im 20. Jahrhundert. Köln: Emons Verlag 2014; 184 S., geb., 24,95 EUR

The German National Reference Centre for Authentic Food (NRZ-Authent) and the competent German food control authorities of the federal states cooperated within the framework of the 10th joint Europol INTERPOL operation OPSON (OPSON X) in the detection of adulterated meat products. A total of 63 meat product samples were collected and analysed by the authorities using standard analytical procedures and subjected to a recently published 16S rDNA metabarcoding analysis. The sequence reads were analysed using 3 bioinformatics data processing strategies. The study aimed to gain additional data on the test samples regarding the authenticity of the declared species and to validate the 16S rDNA metabarcoding method with representative samples. The method was tested not only on 63 test samples, but also on 5 commercial samples from 2 interlaboratory comparison studies and 9 mock mixtures in parallel. The 16S rDNA metabarcoding method was able to detect species that were not target species of the used standard analytical methods, but failed, as shown previously, to detect fallow deer. Otherwise, the qualitative results of the 16S rDNA metabarcoding method were very similar to those of the methods currently in use by the German food control laboratories. Thus, the method has great potential to be used as a screening method for the authentication of mammal and poultry species in meat products.

No AccessDie Eden-AlternativeSilke NachtweySilke NachtweySilke Nachtwey ist Leiterin Pflege und Betreuung des Senioren-Zentrums Krefeld und Landeskoordinatorin der Eden-Alternative.Search for more papers by this authorPublished Online:Mar 2023https://doi.org/10.13109/leid.2023.12.1.34SectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinkedInRedditEmail About Previous article Next article FiguresReferencesRelatedDetails Download book coverVolume 12Issue 1March 2023 ISSN: 2192-1202eISSN: 2196-8217HistoryPublished online:March 2023 PDF download

Die Reihe "Tter Helfer Trittbrettfahrer" 1 mchte sich mit NS-belasteten Persnlichkeiten auf dem Gebiet des heutigen Baden-Wrttemberg auseinanderset-