- Conference Article
- 10.1117/12.3081160
Programmable multimodal microscopy for quantitative phase imaging and auto-labeling in cellular imaging
- Mar 05, 2026
- I-Jan Chen + 6 more +6
In this presentation, we present a novel programmable multimodal microscopy system integrating spatial light modulator (SLM) and programmable optics, enabling real-time imaging across brightfield (BF), darkfield (DF), phase-contrast (PC), differential interference contrast (DIC), and quantitative phase imaging (QPI) without mechanical switching. Precise phase modulation (0~2 π, resolution 0.1 π) allows adaptive optimization of image quality and extraction of quantitative cellular metrics. Leveraging multimodal imaging data, we developed an auto-labeling framework that utilizes morphological and structural information derived from optically fused DF, DIC, and PC images to significantly enhance auto-labeling accuracy and consistency. Additionally, simultaneous acquisition of QPI alongside other phase-sensitive modes (DIC and PC) provides enriched information, greatly improving the detailed monitoring and evaluation of cell cultures. Our results demonstrate the system's capability to enhance precision, automation, and reproducibility in imaging workflows, offering promising applications in advanced cell therapy research, from precise culture monitoring to robust quality assessment.
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