Lingea (Czechia)

Abstract Decoding the genome of cultivated species is one of the key starting points for supporting marker-assisted selection to accelerate breeding programmes. To date, five to six flax genotypes, including fiber flax, linseed and related wild flax, mainly from Canada, China and Russia, have been sequenced and assembled. But no genome assembly was available for Western European flax cultivar, although France is the world's leading producer of fiber flax (85% of world production). The Canadian oilseed flax cultivars CDC Bethune have been mainly used as reference for studies and breeding activities. The best assembly to date was the Chinese fiber Yiya5 assembly, which had the best metric of all the flax genome assemblies available. Recent analyses have shown that up to 30% of fiber flax reads from different origins do not map to the oilseed genome. Thus, much genetic information could be neglected using the oilseed genome as a reference. The aim of this study is to sequence, assemble and annotate the genomes of four European flax cultivars (two fiber Bolchoï and Idéo; two oilseed Marquise and Attila) and provide flax pangenome for genotype characterization and breeding. From 21 to 46 Gb of ONT Minion long reads sequence data were -sequenced for the four genotypes, - assembled using Flye, -polished, validated and organised into hybrid scaffolds using 100 to 197 Gb optical map data (Bionano Genomics), and finally anchored into fifteen T2T pseudomolecules for each genotype using the fiber flax Yiya5 assembly. The final genome size reached 437, 441, 442 and 453 Mb for Idéo, Marquise, Attila and Bolchoï respectively. The four assemblies were annotated using ab initio predictions of genomic sequences and evidence from transcriptomic data. A pangenome graph were constructed for ten cultivars, including the four previous assemblies and six publicly available assemblies (CDC Bethune, Longya10 and Line 3896, Heiya-14, Yiya5 and Atlant). The total pangenome graph size was 835.77 Mbp (74,123 non-redundant orthologous proteins) with the core pangenome size of 172.2 Mbp. The resources generated could be used for the development of genomic tools useful for breeders to improve the efficiency of breeding and variety evaluation.

The atomic register is certainly the most basic object of computing science. Its implementation on top of an n-process asynchronous message-passing system has received a lot of attention. It has been shown that t \textless{} n/2 (where t is the maximal number of processes that may crash) is a necessary and sufficient requirement to build an atomic register on top of a crash-prone asynchronous message-passing system. Considering such a context, this paper visits the notion of a fast implementation of an atomic register, and presents a new time-efficient asynchronous algorithm. Its time-efficiency is measured according to two different underlying synchrony assumptions. Whatever this assumption, a write operation always costs a round-trip delay, while a read operation costs always a round-trip delay in favorable circumstances (intuitively, when it is not concurrent with a write). When designing this algorithm, the design spirit was to be as close as possible to the one of the famous ABD algorithm (proposed by Attiya, Bar-Noy, and Dolev).

Component-oriented and service-oriented approaches have gained a strong enthusiasm in industries and academia with a particular interest for service-oriented approaches. A component is a software entity with given functionalities, made available by a provider, and used to build other application within which it is integrated. The service concept and its use in web-based application development have a huge impact on reuse practices. Accordingly a considerable part of software architectures is influenced; these architectures are moving towards service-oriented architectures. Therefore applications (re)use services that are available elsewhere and many applications interact, without knowing each other, using services available via service servers and their published interfaces and functionalities. Industries propose, through various consortium, languages, technologies and standards. More academic works are also undertaken concerning semantics and formalisation of components and service-based systems. We consider here both streams of works in order to raise research concerns that will help in building quality software. Are there new challenging problems with respect to service-based software construction? Besides, what are the links and the advances compared to distributed systems?