Mantech (Canada)

Abstract Purpose To compare femtosecond laser endothelial graft precut from epithelial and endothelial side, on corneas stored in a new corneal bioreactorMethods Human corneas unsuitable for transplantation for serological reasons and procured by the Auvergne‐Loire eye bank (Saint Etienne, France) were used after informed consent of the relatives, as authorized by French bioethics laws. Femtosecond laser lamellar cuts were realized on corneas stored in a new corneal bioreactor, patented by our laboratory, designed to maintain a constant endothelial pressure and to permit an epithelial aplanation without opening the device. Lamellar cuts of several thickness were realized with corneal epithelium or corneal endothelium side up, but always with epithelial aplanation. OCT images (Casia SS1000, Tome) were realized in the bioreactor before and after each cut. Endothelial viability was determined by using a triple Hoechst/Ethidium/Calcein labeling (HEC, IOVS Pipparelli 2011) coupled with image analysis of the whole endothelial surface immediately after the cut. Surface roughness was assessed by scanning electron microscopyResults This study demonstrates the possibility to realise femtoseconde laser endothelial graft precut directly in a dedicated storage device. First results of endothelial viability in function of lamellar graft thickness and lamellar cut side will be presented as well as surface roughnessConclusion Capacity to realize femtosecond laser endothelial graft precut directly in a storage device could improve the quality and security of delivered lamellar grafts

Phosphorus in runoff from fields where poultry litter is surface-applied is an environmental concern. We investigated the effect of adding phytase and reducing supplemental P in poultry diets and composting poultry manures, with and without Fe and Al amendments, on P in manures, composts, and runoff. We used four diets: normal (no phytase) with 0.4% supplemental P, normal + phytase, phytase + 0.3% P, and phytase + 0.2% P. Adding phytase and decreasing supplemental P in diets reduced total P but increased water-extractable P in manure. Compared with manures, composting reduced both total P, due to dilution of manure with woodchips and straw, and water-extractable P, but beyond a dilution effect so that the ratio of water-extractable P to total P was less in compost than manure. Adding Fe and Al during composting did not consistently change total P or water-extractable P. Manures and composts were surface-applied to soil boxes at a rate of 50 kg total P ha(-1) and subjected to simulated rainfall, with runoff collected for 30 min. For manures, phytase and decreased P in diets had no significant effect on total P or molybdate-reactive P loads (kg ha(-1)) in runoff. Composting reduced total P and molybdate-reactive P loads in runoff, and adding Fe and Al to compost reduced total P but not molybdate-reactive P loads in runoff. Molybdate-reactive P in runoff (mg box(-1)) was well correlated to water-extractable P applied to boxes (mg box(-1)) in manures and composts. Therefore, the final environmental impact of dietary phytase will depend on the management of poultry diets, manure, and farm-scale P balances.

s Submitted for the 68th Annual Scientific Meeting of the American College of Gastroenterology October 10-15, 2003, Baltimore, Maryland: OUTCOMES RESEARCH: PDF Only