Marion General Hospital

Abstract Biopolymer-derived packaging films have gained prominence as eco-friendly substitutes for traditional petroleum-based plastics, driven by escalating environmental concerns. Gallic acid (GA), a naturally abundant polyphenolic compound, exhibits robust antioxidant and antimicrobial properties, positioning it as a pivotal functional additive in biodegradable food packaging systems. Integration of GA into biopolymer matrices enhances mechanical resilience, oxygen barrier efficiency, and oxidative stability, primarily through its role as an oxygen scavenger. These enhancements collectively extend food shelf life while preserving nutritional and sensory quality. Additionally, GA-functionalized films mitigate lipid oxidation and microbial proliferation, thereby elevating food safety standards. Beyond performance improvements, the utilization of GA aligns with global sustainability goals by reducing reliance on non-renewable plastics. This comprehensive review critically evaluates recent advancements in GA-modified biopolymer films, emphasizing their structural-functional synergies, efficacy in food preservation, and contributions to environmentally responsible packaging solutions. Key challenges, including scalability, economic feasibility, and consumer acceptance, are also discussed to guide future research and industrial adoption.

BackgroundHydroxyurea (HU) is an antitumor drug. However, HU exposure is associated with diminished ovarian reserve (DOR). Zi Chong granules, a Chinese Medicine, can protect against DOR, but little is known regarding its underlying mechanisms of DOR treatment, and thus the target of the present study.MethodsSeventy-two female Kunming (KM) mice (4–6 weeks old) were randomly divided into three groups: the control group (Con), the hydroxyurea group (HU), and the Zi Chong group (ZC). The Con group received saline, while the HU and ZC groups were administered hydroxyurea (400 mg/kg/d) by gavage for 21 days to induce diminished ovarian reserve (DOR). Subsequently, the Con and HU groups were given saline, while the ZC group was treated with Zi Chong granules (2.72 g/kg/d) for 15 days. Ovaries and uterus of mice were examined histologically by H&E. The levels of anti-Mullerian hormone (AMH), estradiol (E2), and progesterone (P) were quantified using ELISA kits. The number and quality of oocytes were assessed, and endometrial receptivity was evaluated by immunohistochemistry. 16 S rDNA gene sequencing was used to analyze the composition and abundance of gut microbiome in feces, and non-targeted metabolomics was performed to detect serum metabolite profiles. Correlation analysis was performed to explore the relationships between different gut microbiota and differential metabolites.ResultsZC granules increased the number of primordial follicles in the ovaries, reduced excessive follicular atresia, restored low AMH, upregulated estrogen and progesterone secretion, and increased the number of mature oocytes after ovulation promotion. It also increased thickness of uterine endometrium and the number of glands, resulting in increased endometrial microvessel density (MVD), enhanced endometrial blood supply, reduced CD138 expression levels and endometrial inflammation. HU decreased the abundance of Lactobacillus spp. in mouse intestines and decreased arachidonic acid metabolism, tryptophan metabolism, spermidine and spermine biosynthesis, steroidogenesis, and nicotinate and nicotinamide metabolism. Correlation analysis revealed that HU exerted its side effects by altering the gut microbiome and bacteria-derived metabolites, while ZC granules could reverse DOR partly depends on regulating gut microbiota and metabolites.ConclusionsZC granules may be a potential therapy for alleviating HU-induced DOR.Supplementary InformationThe online version contains supplementary material available at 10.1186/s13048-025-01846-5.

BackgroundInternet health care plays a crucial role in addressing the challenge of distributing high-quality medical resources and promoting the optimal allocation of these resources and health equity in China. Online medical consultation (OMC) plays a more significant role than online health information seeking (OHIS). Currently, the proportion of Chinese patients using OMC is low. Therefore, it is essential to enhance patient engagement with OMC and fully leverage the role of internet health care in optimizing the allocation of medical resources.ObjectiveThis study aims to explore the correlation mechanisms of online medical community users’ switching behaviors from OHIS to OMC.MethodsThis study is based on the knowledge-attitude-practice theory, which combines the social support theory and the health belief model to construct a research model of users’ willingness to transition from OHIS to OMC. The study adopts a questionnaire survey and structural equation modeling method to conduct an empirical study.ResultsGaining knowledge about information support has a significant positive impact on perceived susceptibility (β=.339, P<.001), perceived severity (β=.348, P<.001), and perceived benefits (β=.361, P<.001), while having a significant negative impact on perceived barriers (β=–.285, P<.001). Gaining knowledge about emotional support positively affects perceived susceptibility (β=.220, P<.001) and perceived benefits (β=.149, P<.01) but does not significantly influence perceived severity (β=–.006, P>.05) or perceived barriers (β=.099, P>.05). Perceived susceptibility (β=.123, P<.05), perceived severity (β=.174, P<.001), and perceived benefits (β=.273, P<.001) positively influence patients’ transition to online consultation behavior, whereas perceived barriers (β=–.112, P<.05) negatively impact this switch. In addition, we found that gaining knowledge about information support not only directly affects patients’ behavior in switching to online consultations but also impacts patients’ OMCs through perceived susceptibility (14.23%), perceived severity (13.17%), and perceived benefits (25.28%). In contrast, gaining knowledge about emotional support does not directly influence patient behavior transfer; it operates only through perceived susceptibility (46.95%) and perceived benefit (52.90%).ConclusionsThis study integrated the knowledge-attitude-practice framework, social support theory, and health belief model to uncover the internal logic of patients’ behavioral transfers within online health communities. It confirmed the mediating role of the cognitive-emotional dual-drive pathway and health beliefs. The findings provide a scientific basis for the functional design of online health care platforms and for precise health knowledge dissemination strategies.

Suzetrigine (JOURNAVX) is a novel oral medication approved in January 2025 for the treatment of moderate-to-severe acute pain in adults. It works by selectively inhibiting NaV1.8 voltage-gated sodium channels, which play a key role in pain transmission through peripheral sensory neurons. This targeted mechanism offers potential advantages over current pain management options. Clinical trials have demonstrated efficacy in reducing pain intensity following surgical procedures, making it a promising alternative to opioids. For nurses, suzetrigine represents an important addition to multimodal pain management strategies. Nurses play a key role in educating patients about its use, monitoring for adverse effects, and ensuring adherence to proper dosing guidelines. As suzetrigine becomes more widely available, health care professionals need to understand its benefits, risks, and proper use. This article provides an overview of its mechanism of action, adverse effects, dosing guidelines, cost considerations, and potential role in pain management.

To investigate how the lived experiences of people with progressive supranuclear palsy (PSP) can inform strategies for early and accurate diagnosis, effective interventions, and person-centered clinical trial designs.

Hepatocellular cancer (HCC) progression is facilitated by gene-silencing chromatin histone hypoacetylation due to histone deacetylases (HDACs) activation. However, inhibiting HDACs, an effective treatment for lymphomas, has shown limited success in solid tumors. We report the discovery of a class of HDAC inhibitors (HDACi) that demonstrates exquisite selective cytotoxicity against human HCC cells. The lead compound STR-V-53 (3) showed favorable safety profile in mice and robustly suppressed tumor growth in orthotopic xenograft models of HCC. When combined with the anti-HCC drug sorafenib, STR-V-53 showed greater in vivo efficacy. Moreover, STR-V-53 combined with anti-PD1 therapy increased the CD8+ to regulatory T-cell (Treg) ratio and survival in an orthotopic HCC model in immunocompetent mice. This combination therapy resulted in durable responses in 40% of the mice. Collectively, our data demonstrate that the novel HDACi STR-V-53 is an effective anti-HCC agent that can induce profound responses when combined with standard immunotherapy.

To examine the relationship between blood pressure change and amyloid- and tau-PET abnormalities in cognitively healthy adults.

Surgical site infections (SSIs) post‐cardiothoracic surgery represent a significant challenge in patient care. Understanding the risk factors contributing to SSIs is essential for improving surgical outcomes and patient safety. A comprehensive retrospective analysis was conducted at our institution from January 2021 to December 2022. This study included 30 patients with SSIs and 60 without, following cardiothoracic open surgery. Data were collected on various variables, including hypertension, anaemia, operation time, hospital stay, alcohol consumption, smoking habits, Body Mass Index, age, and drainage tube placement. Univariate and multivariate logistic regression analyses were employed using SPSS software to identify significant predictors of SSIs. Univariate analysis indicated a strong correlation between SSIs and factors like smoking, diabetes mellitus, drainage tube placement, anaemia, and significant intraoperative blood loss (≥800 mL). These factors were statistically significant with p‐values < 0.05. Multivariate logistic regression further confirmed the impact of these factors, with high odds ratios indicating a substantial increase in SSI risk associated with these conditions. This study highlights intraoperative blood loss, anaemia, drainage tube placement, smoking, and diabetes mellitus as key risk factors for SSIs post‐cardiothoracic surgery. Recognising and addressing these factors through targeted preventive measures is crucial in clinical practice to reduce the incidence of SSIs and improve postoperative care in cardiothoracic surgery.

Abstract High-throughput 3’ single-cell RNA-Sequencing (scRNA-Seq) allows for cost-effective, detailed characterization of thousands of individual immune cells from healthy and diseased tissues. Current techniques, however, are limited in their ability to elucidate essential immune cell features, including the variable sequences of T cell receptors (TCRs) that confer antigen specificity in T cells. Here, we present an enrichment strategy that enables simultaneous analysis of TCR variable sequences and corresponding full transcriptomes from 3’ barcoded scRNA-Seq samples. This approach is compatible with common 3’ scRNA-Seq methods, and adaptable to processed samples post hoc. We applied the technique to resolve clonotype-to-phenotype relationships among antigen-activated T cells from immunized mice and from patients with food allergy. We observed diverse but preferential cellular phenotypes manifest among subsets of expanded clonotypes, including functional Th2 states associated with food allergy. These results demonstrate the utility of our method when studying complex diseases in which clonotype-driven immune responses are critical to understanding the underlying biology.