Motif BioSciences (United States)

BackgroundOpioid addiction in the United States has reached epidemic proportions threatening public health. This analysis evaluates the baseline characteristics and bacterial causes of ABSSSI in patients who were IVDU from two parallel Phase 3 trials comparing the treatment of iclaprim with vancomycin.MethodsA total of 621 patients who were IVDU from two parallel Phase 3, double-blind, randomized (1:1), active-controlled, multinational, multicenter trials (REVIVE-1 and REVIVE-2) were analyzed both separately and pooled. This post-hoc analysis summarizes the baseline bacterial causes of ABSSSI identified among IVDU. Per protocol, ABSSSI (major abscesses, cellulitis, or wound infections) were defined as having either the presence of purulent or seropurulent drainage before or after surgical intervention of the wound or at least 3 of the following signs and symptoms: discharge, erythema (extending at least 2 cm beyond the wound edge in any direction), swelling and/or induration, heat and/or localized warmth, and/or pain and/or tenderness to palpation. IVDU was defined based on subjected-reported medical history. At the baseline visit, ABSSSI were sampled for microbiological culture. Cultures were performed locally, and isolates were submitted to the central microbiology laboratory.ResultsAmong IVDU with ABSSSI, average age was 44 years, 67.6% were male, average lesion size was 322 cm2, 10.8% had abnormal renal function (CrCl ≤ 90 mL/minute), and 3.9% had bacteremia. The bacterial causes of ABSSSI among IVDU are shown in the Table.ConclusionIVDU, a growing population, are vulnerable to ABSSSI. S. aureus, including MRSA, and S. anginosus group were the most commonly identified bacterial causes of ABSSSI in patients who are IVDU. Therefore, antibiotic selection should cover these bacteria among IVDU who present with an ABSSSI.DisclosuresAll authors: No reported disclosures.

In this pilot study, the in vitro antimicrobial activity of iclaprim, a diaminopyrimidine, tested in combination with other antimicrobials against recent and common Gram-positive and Gram-negative respiratory pathogens, was examined by the checkerboard method. The range of minimal inhibitory concentrations (MICs) for iclaprim against all bacteria tested in the study was 0.03 to >128 µg ml−1. Iclaprim exhibited synergy with sulfamethoxazole against 11 of the 16 bacterial strains tested, with mean fractional inhibitory concentration index (FICI) values of 0.2–0.5. Synergy with sulfamethoxazole was demonstrated against all Gram-positive bacteria and selected Gram-negative bacteria. Neither synergy nor antagonism was observed for combinations of iclaprim with ampicillin, meropenem, tetracycline, levofloxacin, aztreonam, piperacillin/tazobactam, colistin, cefepime or gentamicin against any of the bacterial strains tested. The significant reduction in the MIC values observed with the combination of iclaprim and sulfamethoxazole demonstrates that this regimen could be effective against common Gram-positive and selected Gram-negative respiratory bacteria.

Acute effect of the proprioceptive neuromuscular facilitation method on vertical jump performance Study aim: To assess the acute effect of the proprioceptive neuromuscular facilitation method (PNF) on the explosive strength of lower extremities of male soldiers. Material and methods: A group of 26 male soldiers performed two exercise sessions on different days, in a random order: without stretching (control exercise) or by applying PNF (experimental exercise). Before and after the PNF (0, 10, 20, 40 and 60 min), 3 vertical counter-movement jumps on a tensometric platform were executed. Results: CMJ results in the experimental sessions were significantly (p<0.001) decreased after the PNF exertion in relation to the initial (pre-session) value. No significant post-session decreases were found in the control exercise. However, the differences between the experimental and control results were significant (p<0.05) only immediately after the PNF exertion and 10 min later. Conclusion: The PNF method negatively affects the vertical jump performance and, thus, ought not to be used as part of warm-ups.

Effects of two kinds of aerobic training on body fat content and serum lipid profile in cadets Study aim: To assess the effects of aerobic training on body fat content and serum lipid profile. Material and methods: A group of 45 male members of Brazilian Military Police Academy, aged 18 - 32 years, were subjected randomly assigned into 3 subgroups and to 12-week programmes: aerobic fatmax zone training (FG; n = 18), traditional military training (TM; n = 15) and non-training control group (C; n = 12). Body fat content (3 skinfolds), serum lipid profile (total cholesterol, LDL, HDL and triglycerides), waist circumference (WC) and O2max (12-min test) were determined. Results: O2max significantly (p<0.05) increased and body fat content decreased in both experimental group compared with the control one. Significant (p<0.05) reductions in WC and LDL vs. control group were observed in the TM group only. Conclusions: The applied aerobic training induced a decrease in percent body fat irrespectively of the kind of training.