Municipality of Tai'an

The cytochrome P450 enzyme is one of the largest proteins family, which has been identified in cuticle biosynthesis, plant growth, development, and stress response. In our study, 28 members of the MdCYP86A family were identified in apple (<italic>Malus</italic> × <italic>domestica</italic> Borkh.). Chromosomal localization analysis found that 28 genes were distributed on 13 chromosomes in apple. Phylogenetic and conserved motifs analysis showed that MdCYP86As in apple is evolutionarily most closely related with <italic>Arabidopsis thaliana.</italic> Each gene contains a cytochrome P450 domain, suggesting that MdCYP86As are highly conserved during the evolution of the species. Cis-acting element analysis of the promoter identified various elements associated with stress response. <italic>MdCYP86A21</italic> was cloned in the apple genome to investigate the function of MdCYP86A family genes. <italic>MdCYP86A21</italic> responded to abiotic stress, including PEG, Nacl, and ABA. The tissue-specific expression patterns revealed that the highest expression tissue was in flower by real-time quantitative reverse transcription (RT-qPCR). Subcellular localization showed that MdCYP86A21 was localized in the endoplasmic reticulum. Further, the function of <italic>MdCYP86A21</italic> to enhanced the resistance to PEG and Nacl stress in apple calli was verified. These results contribute to further study on the function of MdCYP86As and provide a molecular basis for the study in apple stress tolerance.

By introducing 4-amino-1,2,4-triazole (4-NH2-trz), three new polyoxovanadate-based metal-organic frameworks (PMOFs) [Ni3(4-NH2-trz)6][V6O18]·3H2O (1), [Co3(4-NH2-trz)6][V6O18]·3H2O (2) and [Cu3OH(4-NH2-trz)3H2O][VO3]5·H2O (3) have been synthesized and thoroughly characterized by single-crystal X-ray diffraction (SXRD), powder X-ray diffraction (PXRD), infrared spectroscopy (FT-IR), thermogravimetric (TG) analysis and elemental analysis (EA). Among them, PMOFs 1 and 2 had similar structures containing [V6O18]6- clusters; however, PMOF 3 was isolated as a structure containing a [VO3]55- cluster when the amount of the 4-NH2-trz ligand was reduced to half with the other synthesis conditions being the same as those of PMOFs 1 and 2 except for the transition-metal chlorides. Furthermore, the negative charges of polyoxovanadate [V6O18]6- and [VO3]55- anions were balanced by trinuclear complex cations [Ni3(4-NH2-trz)6]6- for 1, [Co3(4-NH2-trz)6]6- for 2 and [Cu3OH(4-NH2-trz)3H2O]5- for 3, respectively. PMOFs 1-3 were further used as heterogeneous catalysts in the Knoevenagel condensation under solvent-free conditions and showed high catalytic activity. PMOF 1 showed moderate catalytic activities in the oxidation of various aromatic alcohols using H2O2 as an oxidant. Moreover, PMOF 1 could be reused at least three times without losing its activity.

As(iii) or arsenite is extremely toxic, and various colorimetric sensors were reported for its on-site detection. A highly cited example was based on gold nanoparticles (AuNPs) modified with a few thiol-containing compounds including dithiothreitol (DTT), reduced glutathione (GSH), and cysteine (Cys). As(iii) was believed to crosslink these surface ligands to aggregate AuNPs and produce a red-to-blue color change. Since As(iii) can also adsorb on AuNPs, we herein carefully studied this effect on these ligand-capped AuNPs. For citrate-capped AuNPs, 10 mM free citrate resulted in a strong blue color in the presence of As(iii) attributable to the elevated ionic strength, while common divalent cations resulted in no color change due to the chelation effect of free citrate. For AuNPs capped with the three thiol compounds, more than 5 mM As(iii) was needed to produce a color change, which was very different from the previously reported color change with nanomolar concentration of As(iii). Our color change was attributed to the displacement of the surface ligands by As(iii) instead of crosslinking by it. This conclusion was made based on the irreversibility of the color change, kinetics of the reaction, and high As(iii) concentration needed. This work has revealed that any two species from AuNPs, thiol and As(iii) can react. It also calls for care in the interpretation of related colorimetric sensing mechanisms, and the need to consider the previously overlooked As(iii) adsorption onto AuNPs.

The fabrication of a CaZnOS shell prevents hydrolysis towards water and acid, and thermal quenching from heat of the red-emitting CaS:Eu2+ core.

目的研究探讨脑电图癔症诱发试验(EEG-HPT)在癔症诊断及鉴别诊断中的价值.方法将符合研究标准的癔症病人120例随机分为试验组60例,对照组60例.进行随机对照试验.结果 EEG-HPT对诊断癔症的敏感性为90.5%,试验组疗效明显高于对照组(P＜0.01).结论 EEG-HPT对癔症的诊断及鉴别诊断是一种安全、可靠、经济、快捷的较为理想的手段.

A study of seasonal fluctuation of serum vitamin A concentrations in children in northern China showed that the prevalence of low serum levels of vitamin A was due to seasonal changes in the intakes of carotenoids. to determine whether plant carotenoids could sustain or improve children's vitamin A status during the fall and winter seasons, we performed an intervention with vegetables starting in the fall in Shandong, China. At a kindergarten, the serum vitamin A concentration was less than 1.05 μmol/L in 39% of the children. for five days per week for 10 weeks, 22 children were each provided with approximately 238 g/day of green and yellow vegetables and 34 g/day of light-coloured vegetables. Nineteen children maintained their customary dietary intake in the fall season, which included 56 g/day of green and yellow vegetables and 224 g/day of light-coloured vegetables. Vitamins A-d 8 and Ad 4 were given before and after the interventions, respectively, and their enrichments in the circulation were determined by gas chromatography/mass spectrometry to investigate vitamin A body stores. the serum concentration of β-carotene improved in the group fed mainly green and yellow vegetables but decreased in the group fed mainly light-coloured vegetables. the serum concentration of retinol was sustained in the group fed mainly green and yellow vegetables but decreased in the group fed mainly light-coloured vegetables (p &lt; .01). the isotope dilution tests confirmed that total body stores of vitamin A were sustained in the group fed mainly green and yellow vegetables but decreased by 27 μmol (7,700 μg), on average, per child in the group fed mainly light-coloured vegetables (p &lt; .06). Dietary green and yellow vegetables can provide adequate vitamin A nutrition to kindergarten children and protect them from becoming vitamin A deficient during seasons when the provitamin A food source is limited.