National Application Center for Pharmaceutical Research

ABSTRACT The status of most fish stocks in Madagascar remains poorly documented. Given their importance for food security and livelihoods, it is necessary to define management measures with reliable data. This study applied a length‐based Bayesian biomass (LBB) estimation method for analysing the fisheries' representative length‐frequency data of seven most commonly caught coral reef fish species in the southwest of Madagascar. Using Froese's indicators, the use of as a stock status indicator, allows a consistent comparison among species in multi‐gear, multi‐species fisheries and provides a practical basis for management advice in data‐limited contexts. The stock status was determined based on median estimates for the period 2018–2023, which provide an assessment of the overall status. Estimates for 2023 were used to describe the most recent status of the stocks. The outputs of this study provide a guide for fisheries managers, coastal communities and authorities to formulate effective management strategies for the sustainability of the marine fisheries in Madagascar. A key recommendation from the study is the need for fisheries managers to improve fisheries data and to periodically monitor biological indicators to assess the status of small‐scale fisheries stocks.

Sentinel animals may play a key role in surveillance of zoonotic arbovirus circulation, particularly in developing countries with low levels of investment in health. This study aimed to assess the relevance of using dogs as sentinel animals for Rift Valley fever virus (RVFV) surveillance in Madagascar. Serological surveys were conducted on 513 dogs and 135 cattle in the Ifanadiana district. In addition, 486 human dry blood samples available from the same area were used. Serostatus against RVFV was determined using a competitive Enzyme-Linked Immunosorbent Assay (cELISA) for dog and cattle samples, and a Multiplex Bead Assay (MBA) for human samples. Serocatalytic models fitted to age-stratified serological data were developed to estimate the RVF force of infection (FOI) under several hypotheses, ranging from no relationship to proportional FOIs between humans, cattle, and dogs. Antibodies to RVFV were detected in 23 of 513 dogs (4.5%; 95% confidence interval (95% CI): [2.9 - 6.7]), in 86 of 486 humans (17.7%; 95% CI: [14.4 – 21.4]), and in 33 of 135 cattle (24.4%; 95% CI: [17.5 - 32.6]). Based on the deviance information criterion, the best supported model indicated that FOI in humans and cattle was proportional to FOI in dogs. Proportionality parameters were estimated at 2.6 (95% credible interval (95% CrI): [1.4 - 5.1]) for humans and 3.5 (95% CrI: [1.3 - 6.4]) for cattle. Our findings suggest that sampling dogs could be used to identify RVFV circulation in endemic areas, and infer the exposure of humans and cattle in these areas in Madagascar. This original result paves the way for an innovative surveillance method for RVFV in Madagascar and other endemic countries, but also for other arboviruses such as West Nile virus.

BackgroundAs of 3 March 2023, Madagascar had reported 1,422 deaths from COVID-19. Up to now, there hasn't been a study to estimate the Total Present Value of Human Life lost TPVHLMADAGASCAR\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\\left({TPVHL}_{MADAGASCAR}\\right)$$\\end{document}, productivity losses, and potential productivity losses averted through COVID-19 vaccination for use in advocacy. The study reported in this paper aimed to fill these information gaps.MethodsThe Human Capital Model (HCM) was used to estimate the TPVHLMADAGASCAR\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$${TPVHL}_{MADAGASCAR}$$\\end{document}, which is the sum of the discounted value of human life losses among individuals in seven different age groups. The Present Value of Human Life for each age group PVHLi\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\\left({PVHL}_{i}\\right)$$\\end{document} was calculated by multiplying the discount factor, the undiscounted years of life, the non-health gross domestic product (GDP) per capita, and the number of COVID-19 deaths in that age group.To test the robustness of the results, the HCM was rerun five times, assuming (i) a 5% discount rate, (ii) a 10% discount rate, (iii) Africa’s highest average life expectancy at birth of 78.76 years, (iv) the world's highest life expectancy of 88.17 years, (v) projected excess COVID-19 mortality of 11,418.66 deaths as of 3 March 2023 in Madagascar, and assuming different levels of vaccine coverage: 100%, 70%, 60.93%, and 8.266%.ResultsThe 1,422 human lives lost due to COVID-19 had a TPVHLMADAGASCAR\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$${TPVHL}_{MADAGASCAR}$$\\end{document} of Int$ 46,331,412; and an average of Int$ 32,582 per human life. Re-estimation of the HCM, using (i) discount rates of 5% and 10% reduced TPVHLMADAGASCAR\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$${TPVHL}_{MADAGASCAR}$$\\end{document} by 23% and 53%, respectively; (ii) average life expectancies of 78.76 years and 88.17 years increased TPVHLMADAGASCAR\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$${TPVHL}_{MADAGASCAR}$$\\end{document} by 23.7% and 39.5%, respectively; (iii) projected excess COVID-19 mortality of 11,418.66 augmented TPVHLMADAGASCAR\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$${TPVHL}_{MADAGASCAR}$$\\end{document} by 703%. Furthermore, it is estimated that vaccinating 70% of the target population could potentially save the country Int$ 1.1 billion, equivalent to 1.94% of the GDP.ConclusionsThe COVID-19 pandemic has resulted in significant health and productivity losses for Madagascar. Optimizing COVID-19 vaccination coverage for the target population could substantially reduce these losses.

The incidence rate of tuberculosis in prisons is estimated to be 8 times greater than that in the general population in Madagascar. Our objectives were to estimate the prevalence of pulmonary tuberculosis and HIV infection among prisoners and to identify risk factors associated with tuberculosis. We conducted a cross-sectional study at the central prison of Antananarivo from March to July 2021. Individual male and female inmates aged ≥ 13 years who had lived in the prison for at least three months prior to the study period were included as participants. Acid-fast bacilli detection by microscopy and/or culture, an intradermal tuberculin test, a chest X-ray, and a rapid diagnostic orientation test for HIV were performed. Among 748 participants, 4 (0.5%) were confirmed to have pulmonary tuberculosis. Overall, 14 (1.9%) patients had “confirmed” or “probable” tuberculosis [0.90–2.84, 95% CI]. The proportion of participants with latent tuberculosis infection was 69.6% (517/743) based on a positive tuberculin test without clinical symptoms or radiography images indicating tuberculosis. Out of 745 HIV screening tests, three showed reactive results (0.4%). Age (OR = 4.4, 95% CI [1.4–14.0]) and prior tuberculosis treatment (or episodes) were found to be associated with confirmed and probable tuberculosis.

Legumes have the ability to establish a nitrogen-fixing symbiosis with soil rhizobia that they house in specific organs, the nodules. In most rhizobium-legume interactions, nodulation occurs on the root. However, certain tropical legumes growing in wetlands possess a unique trait: the capacity to form rhizobia-harbouring nodules on the stem. Despite the originality of the stem nodulation process, its occurrence and diversity in waterlogging-tolerant legumes remains underexplored, impeding a comprehensive analysis of its genetics and biology. Here, we aimed at filling this gap by surveying stem nodulation in legume species-rich wetlands of Madagascar. Stem nodulation was readily observed in eight hydrophytic species of the legume genera, Aeschynomene and Sesbania, for which significant variations in stem nodule density and morphology was documented. Among these species, A. evenia, which is used as genetic model to study the rhizobial symbiosis, was found to be frequently stem-nodulated. Two other Aeschynomene species, A. cristata and A. uniflora, were evidenced to display a profuse stem-nodulation as occurs in S. rostrata. These findings extend our knowledge on legumes species that are endowed with stem nodulation and further indicate that A. evenia, A. cristata, A. uniflora and S. rostrata are of special interest for the study of stem nodulation. As such, these legume species represent opportunities to investigate different modalities of the nitrogen-fixing symbiosis and this knowledge could provide cues for the engineering of nitrogen-fixation in non-legume crops.

Abstract Predicting future conservation needs can help inform conservation management but is subject to uncertainty. We measured deforestation rates during 2015–2017 for 114 protected areas in Madagascar, linked deforestation to the status of protection according to IUCN categories I–VI, used recent deforestation rates to extrapolate forest cover over 2017–2050 and linked the size of forest blocks to the projected persistence of lemur subpopulations. In the six IUCN categories for protected areas in Madagascar the median size of forest blocks is 9–37 km2 and median annual deforestation rates range from 0.02% in the single IUCN category III site to 0.19% in category II and 1.95% in category VI sites. In 2017, 40% of all forest blocks within protected areas were < 10 km2, and this is projected to increase to 45% in 2050. Apart from these small forest fragments, the modal site of forest blocks was 160–320 km2 in 2017, and this is projected to decrease to 80–160 km2 in 2050. The range of > 50% of all lemur species exclusively contains forest blocks of < 10 km2. The modal size of forest blocks > 10 km2 is predicted to remain at 120 km2 until 2050. Although uncertainty remains, these analyses provide hope that forest blocks within the protected areas of Madagascar will remain large enough to maintain lemur subpopulations for most species until 2050. This should allow sufficient time for the implementation of effective conservation measures.

Abstract Background: Malaria remains a significant public health challenge in Madagascar. The World Health Organization (WHO) recommends confirming the presence of parasites through parasitological methods before initiating malaria treatment. Microscopy and rapid diagnostic tests (RDTs) are primary diagnostic methods. This study aimed to evaluate the diagnostic performance of two RDTs: Bioline™ Malaria Ag Pf/Pan and Bioline™ Malaria Ag Pf/Pv by comparing them to microscopic diagnosis, with polymerase chain reaction (PCR) serving as the designated reference standard. Methods: Madagascar-based, from two malaria-endemic districts: Ifanadiana and Mandoto , prospective, diagnostic performance study. Included: anyone, symptomatic and symptomatic, between 3 - 81 years from local primary schools and health canters between 05/2023 and 07/2023. Excluded: patients with severe malaria. Diagnosis: Bioline™ Malaria Ag Pf/Pan and Bioline™ Malaria Ag Pf/Pv RDTs and PCR. Results: The kappa species index was computed using 442 PCR-confirmed positive samples. The sensitivity for P. falciparum detection was 98% (95% CI: 96-99) with Pf/Pan RDTs, yielding a kappa species index of 0.9. Similarly, the sensitivity for P. falciparum detection was 97% (95% CI: 95-99) with Pf/Pv RDTs, accompanied by a kappa species index of 0.9. Pf/PvRDTs demonstrated a sensitivity of 100% (95% CI: 99-100) for P. vivaxdetection, with a kappa species index of 1, indicating strong agreement. Pf/Pan RDTs identified a proportion of 36% of mixed infections. Conclusions: Our findings emphasize the need for careful consideration of the appropriate use of RDTs based on local epidemiology and clinical context. both Ag Pf/Pan and Bioline™ Malaria Ag Pf/Pv RDTs could be used at the peripheral levels where Microscopy and PCR are not possible (CSB1 and CSB 2) and using them according to their availability. At the district level, we could initiate an assessment of geographical cartography which will guide the policy of the use of Ag Pf/Pan and Bioline™ Malaria Ag Pf/PvRDTs according to Plasmodium species identified in different districts. Trial registration: This study is part of the monitoring of quality control of Rapid Diagnostic Test at the National Malaria control Program (NMCP). The clear consent from participating was systematically collected.