National Science and Technology Development Agency

Morphing food refers to food structures designed to undergo controlled shape transformations in response to external stimuli such as heat or moisture, enabling innovative approaches to food design, packaging, and sustainability. Building on this concept, this study investigated squid mantle ( Uroteuthis duvaucelii ) as a natural biological material to determine whether precise surface cuts can produce controlled shape changes under thermal processing conditions. Strips of squid mantle were incised using a semi-automated cutting system at defined cut depths (0, 1, and 2 mm) and cut orientations (0°–90° relative to fibre direction) and subsequently subjected to HWI (hot water immersion) at 85 °C or hot-air drying at 75 °C to activate dynamic shape transformations. Thermal analysis confirmed three denaturation transitions consistent with myosin (∼50 °C), collagen (∼57 °C) and actin (∼74 °C). Cutting depth governed morphing magnitude: uncut strips remained flat, 1 mm cuts produced modest bending (∼30–60°), and 2 mm cuts produced uniform bending in loops or helices (>360°). Orientation determined the deformation mode: longitudinal cuts gave near-planar bending (∼360°), oblique cuts at 45–60° generated helical twisting with large angles (>700°), and 90° cuts largely suppressed global curvature (<20°). HWI resulted in slightly greater curvature than drying, reflecting rapid collagen shrinkage and accelerated water expulsion. Drying kinetics showed an early peak followed by a dominant falling-rate period, indicating internal diffusion control; depth effects exceeded orientation effects when cut depth was fixed at 2 mm. Microscopy and micro-CT analysis indicated that cuts disrupt fibre–collagen continuity, increase local porosity and create stiffness differences that direct anisotropic shrinkage into bending or twisting. These results demonstrate a simple, reproducible approach to predict dynamic shape morphing in natural seafood tissues and while doing so reduce storage and shipping volume, promote sustainability, and reimagine how we design, package, and experience future foods. • First study demonstrating controlled shape transformation in squid mantle tissue. • Semi-automated surface cuts enabled precise and reproducible morphing. • Deeper cut depths (2 mm) induced stronger bending under thermal processing. • Oblique cut orientations (45–60°) redirected shrinkage into twisting. • Fibre anisotropy of squid mantle underpins the overall morphing mechanism.

Severe dengue, marked by plasma leakage, is often linked to secondary heterotypic dengue virus (DENV) infection. While mast cells and basophils contribute to dengue pathogenesis, the role of anti-DENV IgE remains unclear. Here, we investigated whether anti-DENV IgE promotes FcεRI-dependent basophil activation, potentially contributing to disease severity. Plasma from dengue fever (DF, n = 42) and dengue hemorrhagic fever (DHF, n = 56) patients, collected at febrile, defervescence, and convalescent phases, were analyzed using an in-house IgE-capture ELISA developed to detect antibodies against all four DENV serotypes. Functional assays employed RS-ATL8, a human FcεRI-expressing basophil reporter cell line, to assess IgE-mediated activation following DENV antigen cross-linking. The anti-IgE monoclonal antibody Omalizumab was used to evaluate FcεRI dependence. Anti-DENV IgE was detected in both DF and DHF, peaking at defervescence and significantly higher in DHF. Total IgE was elevated but did not differ between groups. About one-third of anti-DENV-IgE-positive plasma samples induced RS-ATL8 activation upon DENV challenge, an effect abolished by omalizumab. These findings indicate a potential pathogenic role of anti-DENV IgE and provide a rationale for further investigation of IgE-targeted interventions.

Contemporary discussions of innovation often privilege human intention and control, overlooking how technologies and environments co-shape the inventive process. This study reconceptualises research commercialisation through a posthuman lens, treating bibliometric software not as a passive analytical tool but as a collaborator in knowledge-making. Drawing on 448 Scopus-indexed publications (2020–2025) connecting posthumanism, innovation, and commercialisation, the analysis employs VOSviewer to visualise conceptual networks and trace temporal shifts within the field. The maps reveal a philosophical and ethical core rooted in posthumanism, new materialism, and ethics. Over time, this core expands into applied domains such as entrepreneurship, gender, and innovation practice. These movements signal the emergence of posthuman innovation ecologies in which human, technological, and ecological agencies intertwine through co-creative processes. The study offers a reflexive lens to reimagine research commercialisation as an ethical and ecological practice within posthuman innovation ecologies.

Early diagnosis of Congenital Hypothyroidism (CH) is critical to prevent irreversible neurodevelopmental damage. However, current TSH-based newborn screening using Dried Blood Spots (DBS) is limited by factors that lead to false-positive and false-negative results, necessitating the development of alternative methods. Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) as a high-throughput solution was investigated. This study presents a novel diagnostic approach utilizing DBS peptide barcoding, which was supported by comprehensive LC-MS/MS and network analysis. Peptide profiles showed a marked and reproducible difference between groups. MALDI-TOF MS identified a unique signature, including six dominant peptides specific to the CH-positive group. Subsequent analysis identified 37 candidate peptides, with network analysis linking 12 key proteins to established CH-related agents (e.g., thyroxine, TSHR). The MALDI-TOF MS peptidomic approach is validated as a robust, rapid, and cost-effective alternative for CH screening. This methodology represents a significant advance toward overcoming the limitations of current TSH assays and establishing a clinically translatable biomarker panel for improved personalized diagnosis of CH.

Nickel titanate (NiTiO3) is a promising pseudocapacitive electrode material; however, its electrochemical performance is limited by low electrical conductivity and slow ion transport. In this work, a solvent-free solid-state NaBH4 reduction strategy is developed to introduce oxygen vacancies (OVs) into NiTiO3, producing reduced NiTiO3 (R-NTO) with a three-dimensional (3D) cauliflower-like morphology and controllable defect density. The optimized R300-NTO-1.2 electrode delivers a 207.8% increase in specific capacitance (505 F g–1) compared with pristine NiTiO3, along with improved wettability and stable cycling performance (89.2% retention after 10,000 cycles), owing to its OV-rich structure and hierarchical porosity. By coupling R300-NTO-1.2 with reduced crumpled graphene oxide (rCGO) as the negative electrode, a high-performance asymmetric supercapacitor (ASC) is constructed, combining Faradaic charge storage at the positive electrode with an electric double-layer capacitance at the negative electrode. Benefiting from effective electrode potential matching, the R300-NTO-1.2//rCGO-ASC operates stably over a 2.0 V voltage window, achieving an energy density of 44.0 Wh kg–1 at 1000 W kg–1 and retaining 34.2 Wh kg–1 at 6000 W kg–1, together with 94.9% capacitance retention and 100% Coulombic efficiency. This study demonstrates that solid-state NaBH4-induced OV engineering, when combined with a structurally robust carbon electrode, offers an effective and scalable approach for developing high-energy, long-life aqueous ASCs.

Ficus hispida L. f. (F. hispida) is commonly used in traditional medicine for various health problems. No comprehensive analysis of all its components has yet been undertaken, despite researchers having explored its chemical constituents and biological activities. Using untargeted metabolomics, we aimed to assess the chemical compositions and metabolic differences among the five parts of F. hispida: bark, fruits, leaves, twigs, and stalks. To discover the compounds that might be accountable for the noted efficacy, our study assessed the correlation between the identified metabolites and anticancer activities. We applied untargeted metabolomics using UHPLC-QTOF-MS/MS to confirm a total of 82 metabolites. These compounds were classified into six phytochemical groups with predominant accumulation in each part: fatty acids and their conjugates (twigs), terpenoids (stalks), phenylpropanoids (bark and twigs), alkaloids (bark and leaves), saccharides and their conjugates (bark), and amino acids and peptides (twigs and stalks). Multivariate analysis showed markedly distinct metabolic patterns across the five tested parts, especially leaf and bark extracts. The sulforhodamine assay (SRB) for the cytotoxicity test revealed that the bark and leaf extracts had the highest potential to inhibit the viability of cholangiocarcinoma (CCA) cells, with IC50 values of 0.71 ± 0.17 µg/mL and 0.82 ± 0.22 µg/mL for KKU-213A, and 0.78 ± 0.13 µg/mL and 1.03 ± 0.21 µg/mL for KKU-055. Univariate analysis revealed that four metabolites, including catharanthine, cianidanol, procyanidin B2, and quinic acid, had significant correlation with these anticancer abilities. Subsequent cytotoxicity studies on these candidate metabolites revealed that catharanthine suppressed CCA cell viability at the IC50 of 41.0 ± 0.99 µM (KKU-213A) and 47.4 ± 4.34 µM (KKU-055) over other candidates. Our research suggests that catharanthine, which is a terpene indole alkaloid found in the bark and leaves of F. hispida, is responsible for the anticancer efficacy against CCA cells.

The emergence of Sundaland during the Pleistocene glaciation has played a crucial role, as the Indo-Pacific Barrier (IPB), in shaping the genetic structure of marine taxa and coastal flora, specifically mangroves. This study investigated the genetic diversity, population structure, demographic history and phylogeography of Avicennia marina and two other Indo-West Pacific (IWP) Avicennia species, Avicennia alba and Avicennia officinalis, across the Andaman Sea (Indian Ocean) and the Gulf of Thailand (Pacific Ocean). Using Restriction-site-Associated DNA sequencing (RADseq), we generated thousands of genome-wide SNPs for 362 Avicennia individuals and revealed a pronounced East-West genetic divergence, separating the Andaman and Gulf of Thailand populations. Phylogeographic and demographic analyses suggest that colonization events by distinct ancestral lineages (Indian and West Pacific Ocean lineages), the Indo-Pacific Barrier (Sundaland), and Pleistocene sea-level fluctuations shaped the population structure and contributed to low genetic diversity (Ho = 0.073-0.083) and high inbreeding coefficients (FIS = 0.169-0.501). This study highlights the importance of Thailand, as part of the Indo-Pacific interface, in harboring genetic resources from both Indian and West Pacific Ocean lineages, as exemplified in A. marina. Consequently, Andaman and Thai Gulf populations should be managed as distinct evolutionarily significant units (ESUs).

Oxidative stress-induced cellular damage is a primary contributor to pathological conditions such as accelerated skin aging. Consequently, there is a strong demand for efficacious antioxidants. Herein, we investigate the cytoprotective properties of Crocodylus siamensis white blood cell extracts and peptides derived from them against H2O2-induced oxidative damage in HaCaT keratinocytes. The extracts show no cytotoxicity, restore cell viability by 3-fold relative to oxidatively stressed cells, and reduce apoptosis. Notably, two enriched peptidesNV10 and RI10demonstrated superior cytoprotective efficacy under oxidative stress. These peptides scavenge intracellular ROS (∼60% reduction), modulate Keap1-Nrf2 pathway components at mRNA levels (downregulating Keap1, upregulating Nrf2), and suppress apoptosis via regulation of caspase and Bcl-2 mRNA expression. Molecular docking predictions suggest that the peptides bind to Keap1, a negative regulator of Nrf2, thereby providing a potential mechanism for Nrf2 pathway activation. These findings suggest that the identified peptides are potential candidates for therapeutic development in skin protection applications.