New Century International Hospital for Children

Inequality of healthcare is one of the hottest social problems in China nowadays since it can affect national social welfare and country development on a huge level. Lots of researchers have discovered the possible problems that cause the inequality and pointed out specific suggestions that try to solve the inequality in the Chinese healthcare system, but a systematic review of the gap in healthcare service is still needed, especially between urban and rural areas. Through collecting and analyzing data from the central government and local governments about the situation of healthcare in both areas, this paper explores the inequality of healthcare between urban and rural areas in the last ten years in China. After researching, the paper finds that the inequality of healthcare exists between rural and urban areas significantly in all aspects of availability, affordability, and support from the government. The main reason might be the different levels of technology and economic development in cities and villages. By investing more funds in developing industries in rural areas, the central government can relieve the significant inequities.

There has been a large increase in the incidence of breast cancer (BC) among women. LINC00473 is a cancer-related lncRNA, participating in the progression of many cancers, but its role in the progression of BC awaits more elaboration. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to quantify LINC00473, miR-424-5p, and cyclin E1 (CCNE1) mRNA expression levels in BC tissues and cells. Cell counting kit-8 (CCK-8) assay was employed to detect the cell viability; the cell migration and invasion abilities were evaluated by the Transwell assay. Western blot and immunohistochemistry (IHC) were adopted to study CCNE1 protein expression; dual-luciferase reporter assay was performed to clarify the targeting relationships among LINC00473, miR-424-5p, and CCNE1. LINC00473 expression was elevated in BC tissues and cell lines, which was associated with lymph node metastasis and higher clinical stage of the patients with BC. LINC00473 proved to be a molecular sponge for miR-424-5p; LINC00473 knockdown impeded the growth, migration, invasion, and epithelial-mesenchymal transition of BC cells, while these effects were abolished by miR-424-5p inhibitors; miR-424-5p targeted CCNE1 to restrain its expression. LINC00473 positively regulated CCNE1 expression, and CCNE1 restoration counteracted the effects induced by LINC00473 knockdown in BC cells. LINC00473 facilitates the progression of BC through miR-424-5p/CCNE1 axis.

The transistor-based biosensors (bioFETs) arrangements have been widely used in early biomarker detection and drug screening. Their non-metalized gate dielectrics is covered by the semiconductor material and can actively transduce the biological changes on the surface. The efficiency of bioFETs to detect a diverse range of various targets is high sensitivity and selectivity. Considerable progress has been attained in designing bioFETs devices, especially for biomedical diagnosis and cell-based assays in the past few decades. With the incorporation of nanotechnology, the performance of bioFETs increased significantly, particularly in respect to the use of nanomaterials such as metal nanomaterials and carbon nanomaterials. In addition, high-quality production of bioFETs on a large-scale, allows it to be one of the most preferred analysis methods. This review presents the basic principle and applications of different types of bioFETs.

The present study aimed to explore the functions and molecular mechanisms of miR-511 in breast cancer. A quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect miR-511 levels in breast cancer tissues; a chi-squared test was used to analyze the relationship between miR-511 expression level and pathological parameters of breast cancer patients; the proliferation of breast cancer cell lines MDA-MB-231 and MCF-7 was determined by the cell counting kit-8 (CCK-8) assay; migration was determined by scratch wound healing assay and transwell assay; TargetScan was used to predict the binding site between the 3'-untranslated region (3'-UTR) of fibroblast growth factor 4 (FGF4) and miR-511; and qRT-PCR, western blot and a luciferase reporter gene assay were conducted to further validate the targeting relationship between miR-511 and FGF4. The expression level of miR-511 was lower in breast cancer tissues than that in adjacent normal tissues. Low expression of miR-511 was associated with larger tumor size, lymph node metastasis and short survival time. In vitro experiments showed that miR-511 modulated the proliferation and metastasis of breast cancer cells. It was also confirmed that miR-511 directly targeted 3'-UTR of FGF4 and reduced its expression, and FGF4 overexpression reversed the effect of miR-511 on the malignant phenotypes of breast cancer cells. The results obtained in the present study demonstrate that miR-511 inhibits breast cancer proliferation and metastasis by down-regulating FGF4 expression, which may be helpful in the development of new treatment strategies for breast cancer.

Objective To study the threshold values of lactic acid (Lac)and buffuer excess (BE) when neonatal umbilical arterial blood gas is at pH <7.20. Methods A total of 2 518 pregnant women admitted in Beijing New Century Women and Children′s Hospital from January 2017 to February 2019 were enrolled.The general conditions in perinatal period and labor methods were counted.Umbilical cord arterial blood was immediately collected before the first cry of newborn and was detected on the Abbott I-STAT automatic blood gas analyzer from the United States, and then the umbilical arterial blood gas was measured.We analyzed the perinatal factors affecting umbilical arterial blood gas.We studied the threshold values of Lac and BE for neonal asphyxia by receiver operating characteristic curve. Results (1)Maternal anemia, Streptococcus agalactiae infection, precipitate labour, placenta abnormalities had no significant impact on neonatal umbilical artery blood gas.The Lac value in blood gas was higher in the group of premature rupture of fetal membranes and fetal intrauterine distress.The value of BE was lower in the group with premature rupture of fetal membranes and fetal distress (P 3.97 mmol/L, the sensitivity and specificity of the diagnosis of asphyxia were 0.864 and 0.791, respectively.When BE was ≤-6 mmol/L, the diagnostic sensitivity and specificity of asphyxia were 0.613 and 0.756, respectively. Conclusion Neonatal umbilical arterial blood gas is affected by many factors.The effect of accouche on umbilical arterial blood gas is large.When there is asphyxia with pH 3.97 mmol/L and ≤-6 mmol/L, respectively. Key words: Umbilical artery; Blood gas analysis; Lactic acid; Buffuer excess; Newborn

Congenital pulmonary vein atresia (PVA) is a rare anomaly in clinic.Patients with congenital PVA are usually symptomatic and present with recurrent episodes of pneumonia or haemoptysis in infancy or childhood.Because PVA often occurs in the setting of other congenital heart lesions, so repeated infection of lung might be consi-dered to associate with left-to-right intracardiac shunt with increased pulmonary blood, but ignore further examination of more rare cause.Diagnosis of PVA is based primarily on imaging test, chest radiograph, lung CT and fiberoptic bronchoscopy might reveal some indirect characteristic changes of the disease.Three dimensional contrast enhanced cardiac CT can make a definite diagnosis.The disease is difficult to cure at present, the aim of the treatment is to preserve lung function.The prognosis is associated with the number of involved pulmonary veins. Key words: Pulmonary vein atresia; Congenital heart disease; Pneumonia; Haemoptysis