Northwest Vista College

Diabetic retinopathy (DR) causes vision loss due to sustained inflammation and vascular damage. The vascular damage is evident by fibrinogen leakage, angiogenesis, and hypoxia. Neuronal regulation of microglia via the CX3CL1 (Fractalkine or FKN)-CX3CR1 pathway plays a significant role in retinal pathology. Defects in FKN or CX3CR1 exacerbate inflammation, vascular damage, and vision impairment. However, the contribution of hypoxic astrocytes to the pathological process of DR is unclear. A hypoxic model (7 days of systemic 7.5% O2) was utilized to induce retinal damage in adult mice in the absence of systemic inflammatory signals. This model induced vascular and microglial responses similar to 10 weeks of STZ-induced hyperglycemia. The goal of this study is to characterize retinal damage in WT and mice with defects in the FKN-CX3CR1 signaling axis and hence assess the impact of the microglial inflammatory responses to hypoxic retinopathy. Tissues were analyzed by immunostaining, RNA sequencing, and cytokine quantification. We found that CX3CR1 deficiency in hypoxic animals induced reactive astrogliosis and that Müller glial responses to hypoxia and systemic inflammation were dependent on FKN signaling. Exacerbated microglial reactivity to hypoxic conditions significantly altered the expression of HIF transcripts. Microglial dysregulation was found to reduce the anti-inflammatory response to hypoxic conditions, downregulate hypoxia-responsive gene expression, and restrained LPS-induced inflammatory responses. We found that microglia dysregulation alters the hypoxic response by inhibiting the upregulation of HIF2α/3α, increasing CD31 immunoreactivity, and altering the expression of ECM-associated transcripts such as type I, III, and XVIII collagens to hypoxic conditions.

Introductory courses in biology often act as a gateway for students seeking careers in healthcare and science-related fields. As such, they provide a prime entry point for innovations seeking to enhance students’ learning of foundational content. Extant innovations and interventions have been found to positively impact students’ study strategy use with concomitant impacts on course exams and grades. These innovations, however, often have associated time and other costs, which may ultimately limit more widespread use. Our study builds on prior findings by exploring the extent to which students evidence increased use of effective study strategies after engaging in a brief (i.e., 15-min), online module requiring no financial cost for students or time commitment from instructors, and whether changes in students’ use of effective study strategies are associated with changes in exam performance. The present study employed a brief, online module designed to support undergraduate students’ (n = 98) use of effective study strategies in an introductory human anatomy and physiology course. Through a pretest-posttest design, students described the strategies they used to study and completed four cognitive and metacognitive subscales before and after engaging in a brief, online module designed to teach them about effective study strategies. Results were somewhat mixed: students evidenced a modest, statistically significant increase in the number of strategies used and changes in strategy use were associated with changes in exam score only for some measures. Notably, this relationship was not moderated by GPA, suggesting that the strength of the relationship between changes in strategy use and changes in exam scores were not different depending on students’ levels of prior academic performance. Taken together, the innovation was associated with increases in students’ exam scores, irrespective of GPA, but future research should explore the refinement and extension of the innovation to explore ways that increase efficacy and impact while still balancing sustainable implementation to account for challenges associated with instructor supervision and training, financial costs, and students’ time.

The naked mole-rat (Heterocephalus glaber) has fascinated zoologists for at least half a century. It has also generated considerable biomedical interest not only because of its extraordinary longevity, but also because of unusual protective features (e.g. its tolerance of variable oxygen availability), which may be pertinent to several human disease states, including ischemia/reperfusion injury and neurodegeneration. A recent article entitled ‘Surprisingly long survival of premature conclusions about naked mole-rat biology’ described 28 “myths” which, those authors claimed, are a “perpetuation of beautiful, but falsified, hypotheses” and impede our understanding of this enigmatic mammal. Here, we re-examine each of these “myths” based on evidence published in the scientific literature. Following Braude et al., we argue that these “myths” fall into four main categories: (1) “myths” that would be better described as oversimplifications, some of which persist solely in the popular press; (2) “myths” that are based on incomplete understanding, where more evidence is clearly needed; (3) “myths” where the accumulation of evidence over the years has led to a revision in interpretation, but where there is no significant disagreement among scientists currently working in the field; (4) “myths” where there is a genuine difference in opinion among active researchers, based on alternative interpretations of the available evidence. The term “myth” is particularly inappropriate when applied to competing, evidence-based hypotheses, which form part of the normal evolution of scientific knowledge. Here, we provide a comprehensive critical review of naked mole-rat biology and attempt to clarify some of these misconceptions.

BackgroundSupportive male involvement is strongly correlated with contraceptive use. In Rwanda, where the contraceptive prevalence rate among married women increased from 17 to 52% from 2005 to 2010, and stagnated at 53% in 2015, understanding the role of male partners in collaborative couple contraceptive use can help inform programs designed to further increase the use of contraception in Rwanda.MethodsThis study utilized qualitative methods in 2018, specifically 32 in-depth interviewers with mostly current users of modern contraceptive methods and eight focus group discussions with family planning providers—both family planning nurses and community health workers (CHWs). Respondents were from Musanze and Nyamasheke Districts, the districts with the highest and lowest modern contraceptive use, respectively, to explore the role of couple collaboration in family planning use in Rwanda. Data were analyzed using the thematic content approach in Atlas.ti (8).ResultsFindings demonstrate that some men are opposed to use of male methods of contraception, and some are opposed to any contraceptive use, which can lead to covert use. Women and providers prefer collaborative couple contraceptive use—as a result, providers advocate for and encourage male partner participation in contraceptive use. Women are most often burdened with seeking out information, initiating discussions, and sharing information discovered about contraceptive use with partners. Decision-making about contraceptive use, once discussed, can be collaborative and motivated by financial considerations. When couple contraceptive use is collaborative, benefits range from marital harmony to husband’s support of sustained use through reminders about appointments, joint counseling, and support in managing side effects.ConclusionFamily planning providers at the community and clinic levels encourage collaborative contraceptive use among couples and some Rwandan couples communicate well about family planning use. Despite the positives, women are expected to source family planning information, share that information with their male partners, seek out family planning services, and use family planning. If more Rwandan male partners accepted use, used male methods of contraception, and participated even more in the work it takes to use family planning, the potential for sustained, and even enhanced, contraceptive use in Rwanda could be realized.

Abstract Background: Contraceptive discontinuation is a common event. Family planning programs often focus more on recruitment of new users as opposed to maintaining use among current users. A focus on sustaining users is imperative for long-term family planning program success.Methods: This qualitative study in Rwanda in 2018 included eight focus group discussions with 88 family planning providers and 32 in-depth interviews with contraceptive users. The data were collected in the two districts with the highest and lowest rates of contraceptive use, Musanze and Nyamasheke, respectively. The aim of this study is to better understand how providers and contraceptive users in Rwanda navigate one of the greatest challenges to family planning programs: contraceptive discontinuation.Results: Family planning providers and current users in Rwanda do not consider method discontinuation an option. Providers give support and medicine for side effects for continuers and counseling for those opting to switch. Current users are willing to try many methods until they find the right one for them – and once they find the right method, they plan to, or use the method, for long durations.Conclusions: The Rwandan family planning program is primed to meet the needs of users in their sustained use of contraceptives through empathetic and responsive counseling. Concurrently, family planning users are determined to use family planning to meet their desired family size goals. The matching goals of both providers and clients indicates that contraceptive use will likely continue to increase in Rwanda. Due to long periods of pregnancy prevention desired by users the increased use of long acting methods should be encouraged.

Abstract Background High fertility rates pose health risks to both mothers and children and impede economic growth; therefore, family planning use is vital to achieving sustainable population growth and to help build thriving communities. Contraceptive use in Rwanda has tripled since 2005. This study aims to understand the role of coordinated and integrated family planning service delivery in achieving this unparalleled success in Rwanda. Methods This qualitative study in 2018 included eight focus group discussions with family planning providers and 32 in-depth interviews with experienced family planning users. Results Results indicate a well-coordinated family planning service delivery system with community health workers and nurses filling different and complementary roles in meeting family planning client needs at the local level. In addition, integration of family planning into other maternal and child health services is the norm. Conclusions The coordination and integration of family planning across both providers and services may help explain the extraordinary increase in Rwanda’s contraceptive usage, and has potential applications for enhancing family planning service delivery in other settings as well.

Water and wastewater industry leaders in Texas and throughout the United States have expressed concern over high rates of retirement eligibility and difficulties finding and attracting workers ready to fill job openings, especially for work in smaller systems. In late January 2018, the U.S. Government Accountability Office released a report on water workforce readiness and a bill was introduced in the U.S. Senate to establish a water infrastructure workforce development program. Concern over existing education of workers in water and demographic information projecting future workforce readiness are commonly cited as signaling a coming crisis for the water industry. An alignment of post-secondary training and industry needs is recommended to meet coming workforce employment requirements for Texas and the nation. A model post-secondary education pathway for water science and technology is described to support water workforce readiness. Citation: Rosen RA, Cifuentes LA, Fischer J, Marquise H, Tracy JC. 2018. Water security for Texas: a post-secondary education pathway for water workforce readiness. Texas Water Journal. 9(1):120-128. Available from: https://doi.org/10.21423/twj.v9i1.7078.

This lesson presents an interrupted case study based on the true story of the 2002 murder of Christa Worthington in Massachusetts. The case was developed for use in an undergraduate non-majors life science course, but would also be appropriate for a high school biology course or a forensic science course. During this lesson, students examine a crime scene diagram and discuss evidence collection. Students then conduct a hands-on activity extracting DNA from wheat germ to simulate how DNA would be isolated from crime scene samples. Lastly the students will analyze simulated DNA profiles produced using STRs, polymerase chain reaction, and gel electrophoresis to help match a crime scene sample to one of five suspects. The pros and cons surrounding the use of a DNA dragnet are also discussed.

The growing need for biomedical contrast agents has led to the current development of multi-functional materials such as lanthanide-based nanoparticles (NPs). The optical and magnetic properties these nanoparticles (NPs) possess are important to enhance current biomedical imaging techniques. To increase the optical emissions of the nanoparticles, neodymium (Nd3+) and ytterbium (Yb3+) were introduced into a magnetic host of NaGdF4. The energy transfer between Nd3+ and the Yb3+ was then investigated at multiple concentrations to determine the optimal dopant levels. The NaGdF4:Nd3+,Yb3+ nanoparticles were synthesized through a modified solvothermal method, resulting in rectangular structures, with an average side length of 17.87 ± 4.38 nm. A double dopant concentration of 10% Nd3+ and 4% Yb3+ was found to be optimal, increasing the emission intensity by 71.5% when compared to the widely used Nd3+ single dopant. Decay measurements confirm energy transfer from Nd3+ to Yb3+, with a lifetime shortening from Nd3+ 1064 nm emission and a calculated lifetime of 12.72 ms with 98% efficiency. Despite NaGdF4:Nd3+,Yb3+ NPs showing a slight decrease in their magnetic response at the expense of optimizing optical emission, as it is directly dependent on the Gd3+ concentration, a strong paramagnetic behavior was still observed. These results corroborate that NaGdF4:Nd3+,Yb3+ NPs are viable candidates for multimodal imaging.

This paper provides a review of the practices and tensions informing approaches to professional development for early career academic geographers who are teaching in higher education. We offer examples from Britain, Canada, Nigeria and the USA. The tensions include: institutional and departmental cultures; models that offer generic and discipline-specific approaches; the credibility of alternative settings for professional development in teaching and learning; the valuing of professional development and of teaching in academic systems of reward and recognition; and the challenges of balancing professional and personal life. We summarize concepts of good practice and suggest opportunities for future research.