Novozymes (United States)

Oral malodor negatively impacts a person's quality of life and may affect up to 50% of the population. The aim of this randomized, placebo and no-product controlled, evaluator-blind, proof-of-concept study was to evaluate the effectiveness and safety of the single use of two experimental lozenges containing the laccase enzyme and green coffee extract (with and without flavor) in reducing intrinsic oral malodor. Following 12-16 h of avoidance of oral hygiene,156 generally healthy subjects presented at screening and baseline visits with a mean organoleptic odor intensity (OI) score of ⩾2 and an OralChromaTMreading of ⩾125 parts per billion (ppb) hydrogen sulfide (H2S) gas and were randomly assigned to receive either one of the two experimental lozenges, a placebo lozenge, or no-product. Following the supervised use of the assigned products, subjects' oral malodor was evaluated using OI assessments and OralChromaTMmeasurement for volatile sulfur compounds (VSCs) immediately following product use (approximately 5 min), and at 30 min, 1 h, 2 h, 3 h and 4 h. The two experimental lozenges, with and without flavor, showed significant reductions in OI scores compared with the placebo and no-product groups at all time points (p< 0.001). At 5 min post-product use, the experimental lozenges, with and without flavor, were significantly better than the no-product group in reducing the VSCs (p< 0.04). The results of individual VSC components (hydrogen sulfide, methyl mercaptan and dimethyl sulfide) were variable; both experimental lozenges notably reduced hydrogen sulfide and methyl mercaptan levels in most post-use assessments. Four minor adverse events were reported, none of which were directly linked to the product. In conclusion, the experimental lozenges, whether flavored or not, were safe and effective in reducing oral malodor over a span of 4 h, based on organoleptic OI scores.Clinical Trial No: NCT05950529.

According to the US Environmental Protection Agency, around 11 million tons of post-consumer textile waste (PCTW) are disposed in U.S. landfills annually, which is 8% of all municipal solid waste. PCTW is landfilled because it contains complex blends of natural and synthetic fibers that are not easy to separate, and dyes and finishing chemicals on the fabrics interfere with recycling. The goal of this work was to develop a laboratory scale process for deconstructing and separating cut fabrics into different fiber fractions to create purified product streams that could promote textile recycling. Method parameters were selected from preliminary tests on various fabric types, followed by parametric evaluation with a set of rationally prepared model textile wastes. The combination of aggressive mechanical agitation together with cellulase catalyzed hydrolysis caused 100% cotton fabrics to disintegrate completely into a slurry of < 2 mm small solids and water soluble degradation products. The presence of reactive dyes on the model fabrics inhibited degradation, with the bifunctional reactive dye creating larger barriers to degradation than the monofunctional dye. Dye induced barriers were overcome with sufficient time, enzyme amount, and repeated treatment. Even though its collateral impact was a decrease in initial fabric burst strength, the presence of durable press (DP) finish on cotton presented a large obstacle to enzymatic degradation. This was overcome by including acid/alkali pretreatments to DP fabric before applying enzyme. The presence of polyester fiber in a cotton/polyester blend caused the fabric to retain its macroscopic knitted structure, while enzymatically degraded cotton was removed by washing and filtration to yield clean polyester. In all cases, fabric degradation products were separated by filtration into – depending on the severity of the treatments – residual large solids and small solids fractions and a clarified process liquid that contained soluble components. These three fractions were quantified gravimetrically and were characterized using high-performance liquid chromatography (HPLC), x-ray diffraction (XRD), differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FTIR), viscometry, scanning electron microscopy (SEM) and optical microscopy. The small solids present in the slurries after cotton degradation could be valuable as additives for paper, composites and other products, while the glucose-rich process syrups could be used to produce fuels and chemicals by fermentation, all of which would help divert PCTW from landfills. Importantly, even when cellulosic textile components were not fully degraded to soluble compounds, their conversion to pumpable slurries enabled easy handling of the degraded material and allowed recovery of non-degraded synthetic fibers by simple filtration and washing.

Enzymatic transesterification of fatty acid oils to fatty acid methyl esters (FAME) is a wellestablished process involving an enzymatically catalyzed transesterification reaction step with yields greater than 97%. The unreacted residual consists primarily of free fatty acids (FFA), which contribute to the acid value (mg KOH/g biodiesel) and are typically 2–3 wt%. Water is critical to the stability and activity of the enzyme employed in the transesterification reaction. Consequently, reducing the residual FFA further is not possible with the existing process.

The decomposition of lignocellulose in nature, as well as when used as feedstock in industrial settings, takes place in a dynamic system of biotic and abiotic reactions. In the present study, the impact of abiotic reactions during the storage of pretreated lignocellulose on the efficiency of subsequent saccharification was investigated. Abiotic decarboxylation was higher in steam-pretreated wheat straw (SWS, up till 1.5% CO2) than in dilute-acid-catalysed steam-pretreated forestry residue (SFR, up till 3.2% CO2) which could be due to higher iron content in SFR and there was no significant CO2 production in warm-water-washed slurries. Unwashed slurries rapidly consumed O2 during incubation at 50 °C; the behaviour was more dependent on storage conditions in case of SWS than SFR slurries. There was a pH drop in the slurries which did not correlate with acetic acid release. Storage of SWS under aerobic conditions led to oxidation of the substrate and reduced the extent of enzymatic saccharification by Cellic® CTec3. Catalase had no effect on the fractional conversion of the aerobically stored substrate, suggesting that the lower fractional conversion was due to reduced activity of the lytic polysaccharide monooxygenase component during saccharification. The fractional conversion of SFR was low in all cases, and cellulose hydrolysis ceased before the first sampling point. This was possibly due to excessive pretreatment of the forest residues. The conditions at which pretreated lignocellulose are stored after pretreatment significantly influenced the extent and kind of abiotic reactions that take place during storage. This in turn influenced the efficiency of subsequent saccharification. Pretreated substrates for laboratory testing must, therefore, be stored in a manner that minimizes abiotic oxidation to ensure that the properties of the substrate resemble those in an industrial setting, where pretreated lignocellulose is fed almost directly into the saccharification vessel.

Abstract BackgroundThere is an urgent need for early screening diagnostics for Alzheimer’s disease (AD), cases continue to increase without effective diagnosis and treatment solutions. There is no test to diagnose AD at its earliest stages in healthy asymptomatic individuals to determine disease status. Fluid biomarkers, particularly blood‐based, could have the capacity to be widely implemented as diagnostic screens and to rule‐in/rule‐out further invasive and expensive testing. Amyloid‐beta (Aβ) oligomers continue to be a compelling target for therapeutics and diagnostics, are present in blood and are bound by blood cells. Given the accumulated evidence that Aβ oligomers are present in blood; we are developing a novel early detection blood cell‐based biomarker test using proprietary peptide ligands before any signs of AD in healthy asymptomatic individuals, much like a cholesterol blood test for cardiovascular disease.MethodOur technology, Pronucleon™ peptides, involves custom‐synthesized peptides that mimic the misfolding region of proteins such as Aβ. We’ve established specific, sensitive and selective detection of Aβ oligomers with our peptides using spectroscopic techniques. For this study we measured the binding capacity index (BCI) of blood cells using the following assay: diluted whole blood was incubated with or without our proprietary stabilized Aβ oligomer; fluorescently‐labeled Pronucleon™ peptide was then incubated with the blood samples followed by flow cytometric analysis. We compared the fluorescence intensity of each sample, one with and one without the stabilized Aβ oligomer. The assay was performed on 60 clinical samples (n=20 for each Control, MCI and AD) followed by statistical analysis to confirm any differences within and between the patient groups.ResultPronucleon™ peptides displayed specificity and sensitivity for Aβ oligomers; peptides also showed increased binding to AD blood cells as compared to normal; our cell‐based flow cytometry assay exhibited differentiation in BCI scoring between healthy and AD samples.ConclusionThese results set the stage for further development of an exciting innovative approach toward a screening tool for diagnosing Alzheimer’s disease, allowing the primary care setting in the medical community, the AD therapeutic community and the public an opportunity to address early detection of this disease before its symptoms appear.

Trichoderma reesei's potential as a rapid and efficient biomass degrader was first recognized in the 1950s when it was isolated from Army textiles during World War II. The microbe secreted cellulases that were degrading cotton-based tents and clothing of service members stationed on the Solomon Islands. In the 1970s, at the time of the first global oil crisis, research interest in T. reesei gained popularity as it was explored as part of the solution to the worlds growing dependence on fossil fuels. Much of this early work focused on classical mutagenesis and selection of hypercellulolytic strains. This early lineage was used as a starting point for both academic research with the goal of understanding secretion and regulation of expression of the complex mixture of enzymes required for cellulosic biomass decay as well as for its development as a host for industrial enzyme production. In 2001, at the onset of the second major oil crisis, the US Department of Energy supported research programs in microbial cellulases to produce ethanol from biomass which led to another surge in the study of T. reesei. This further accelerated the development of molecular biology and recombinant DNA tools in T. reesei. In addition to T. reesei's role in bio-ethanol production, it is used to produce industrial enzymes with a broad range of applications supporting the bio-based economy. To date there are around 243 commercially available enzyme products manufactured by fermentation of microorganisms; 30 of these are made using Trichoderma as a host, 21 of which are recombinant products sold for use in food, feed, and technical applications including textiles and pulp and paper.

Camelina sativa (L.) Crntz. is a hardy self-pollinated oilseed plant that belongs to the Brassicaceae family; widely grown throughout the northern hemisphere until the 1940s for production of vegetable oil but was later displaced by higher-yielding rapeseed and sunflower crops. However, interest in camelina as an alternative oil source has been renewed due to its high oil content that is rich in polyunsaturated fatty acids, antioxidants as well as its ability to grow on marginal lands with minimal requirements. For this reason, our group decided to screen the existing (2011) National Genetic Resources Program (NGRP) center collection of camelina for its genetic diversity and provide a phenotypic evaluation of the cultivars available. Properties evaluated include seed and oil traits, developmental and mature morphologies, as well as chromosome content. Selectable marker genes were also evaluated for potential use in biotech manipulation. Data is provided in a raw uncompiled format to allow other researchers to analyze the unbiased information for their own studies. Our evaluation has determined that the NGRP collection has a wide range of genetic potential for both breeding and biotechnological manipulation purposes. Accessions were identified within the NGRP collection that appear to have desirable seed harvest weight (5.06 g/plant) and oil content (44.1%). Other cultivars were identified as having fatty acid characteristics that may be suitable for meal and/or food use, such as low (<2%) erucic acid content, which is often considered for healthy consumption and ranged from a high of 4.79% to a low of 1.83%. Descriptive statistics are provided for a breadth of traits from 41 accessions, as well as raw data, and key seed traits are further explored. Data presented is available for public use.

Core Ideas Soybean seed inoculation with Bradyrhizobium japonicum enhances grain production. Greater inoculation response happens in Argentinean sites than in the United States. Several soil properties and crop management practices are related with the responses to inoculation. Although the relevance of biological N nutrition of soybean [Glycine max (L.) Merr.] is recognized worldwide, inoculation with Bradyrhizobium japonicum shows variable results and the benefit needs to be validated under current crop production practices. We conducted statistical analysis of soybean field trial data to provide insight into factors affecting the efficacy of soybean inoculation under contrasting crop production conditions. Most experimental sites, 187 trials in the United States and 152 trials in Argentina, were in soils with soybean history and naturalized B. japonicum strains. Yield increases were greater in Argentina (190 kg ha−1 equivalent to 6.39%) than in the United States (60 kg ha−1 equivalent to 1.67%). Tillage methods did not affect inoculant performance. In the United States, inoculation was more effective in soils with higher pH (&gt;6.8) while in Argentina the greatest inoculation effect on crop production occurred in soils with a lower pH (&lt;5.5). In the United States, where most of the trials were in rotation with corn (Zea mays L), the greatest positive effect of inoculation was observed in late planted soybean crops and independent of soil organic matter (SOM). In Argentina, the inoculant had its greatest effect in soils with no soybean history, a relatively high SOM, higher levels of soil extractable P and S, and in areas with greater precipitation during early reproductive growing stages. In both regions, the yield increases due to B. japonicum inoculation support the regular use of this practice to help provide adequate conditions for soybean production.