O2 Czech Republic (Czechia)

The protein design field is rapidly advancing, with frequent emergence of new models and pipelines for designing de novo proteins with tailored properties and functions not found in nature. However, the current tool landscape is fragmented, tools are hard to install and deploy, and require significant computational expertise to integrate into end-to-end, scalable pipelines. A particular challenge is managing many sequences, structures, and metrics for downstream testing and retrospective analysis of input parameters. To address this need, we introduce Ovo, an open-source de novo protein design ecosystem that consolidates models, workflows, data management, and interactive visualization into a scalable, infrastructure-agnostic platform. Ovo features Nextflow-based workflow orchestration, a storage layer, and both command-line and graphical interfaces that democratize scaffold design, binder design and diversification, and validation workflows. Ovo's novel ProteinQC module computes comprehensive sequence and structure descriptors, contextualizing designs against reference sets. Ovo plugins let the community add new workflows and user interfaces to accelerate adoption of emerging methods and facilitate community-driven benchmarking. Ovo lowers engineering barriers and demystifies the design process, allowing experts and non-technical users to design proteins at scale. With community-driven development, Ovo can accelerate de novo protein design and advance discovery in therapeutics and biotechnology.

<bold>Background:</bold> The treatment landscape of SSc-ILD has changed over the past decades. <bold>Objective:</bold> Evaluate current treatment regimens and identify factors associated with these strategies. <bold>Methods:</bold> SSc-ILD patients from the EUSTAR database starting immunesuppresive and/or anti-fibrotic therapies ≥2017 were included. Treatment regimens, including mono and combination therapies, switch and discontinuation and factors associated with treatment decisions, were analyzed using multivariable logistic regression. <bold>Results:</bold> Among 277 patients, 159 (57.4%) were started on treatment: 377 regimens with mycophenolate mofetil, 186 with methotrexate, 257 with rituximab and 122 with tocilizumab were identified, Figure 1 summarized combinations. 14% of regimens were switched and 24% were discontinued. Factors associated with combination therapies included younger age (OR 0.97, p<0.001), lower %pDLCO (OR 0.98, p=0.008), and diffuse SSc (OR 1.77, p=0.009). Switching therapies was associated with diffuse SSc (OR 1.90, p=0.038) and arthritis (OR 2.97, p=0.012); no factors were identified for discontinuation. <bold>Conclusion:</bold> Currently, the majority of SSc-ILD patients are treated with an immunosuppressive agent, often in combination regimes. Multiorgan manifestation significantly impact treatment choices. Figure 1. Treatment regimens in SSc-ILD: A mycophenolate mofetil-; B methotrexate-; C rituximab-,D tocilizumab -based regimens. <fig><object-id>erj;66/suppl_69/PA4060/F1</object-id><object-id>F1</object-id><object-id>F1</object-id><graphic></graphic></fig>

Transgerational transmitted trauma is the transmission ofpsychological injuries between generations. This article uses two case vignettes to explore selected schema therapy approaches that help clients process transgenerationally transmitted trauma from their ancestors. Specific methods of imagery rescripting and chair work enable clients to transform maladaptive patterns ofexperiencing into healthier coping strategies, support better stress management, improve emotional regulation and communication in relationships, and encourage more profound relationships with themselves and others. Two case studies illustrate imagery rescripting and chair work, in which the client takes the role of their traumatised ancestor. The first case shows a schema therapy of a young woman struggling with repressed emotions related to her family history and the suicide of her grandfather. The second case demonstrates therapeutic work with a client struggling with emotional outbursts and self-harm that are a reflection of transgenerational traumatisation passed down from her mother and grandparents. Both clients experienced a significant reduction in borderline symptoms during the therapeutic work. At the same time, these clients progressed in understanding the inherited transgenerational family patterns and improved their behaviour towards themselves and others. Two case examples have shown that experiential interventions such as imagery rescripting and chairwork can help clients process transferred patterns of traumatic experience and behaviour and bring adaptive changes into their lives. Imagery rescripting as atherapeutic tool can bridge the emotional and physical aspects of transferred learned experiences and help clients integrate a new perspective on themselves and others. Imagery rescripting and chairwork can be effective therapeutic tools for addressing transgenerational trauma.

Introduction and Objective: Glycemia management early after liver transplantation (LTx) is challenging due to patients' critical state and corticoid therapy. We evaluated the impact of actively used real-time continuous glucose monitoring (CGM) on glycemic control in LTx recipients at postoperative ICU in a prospective randomized trial (clinicaltrials.gov NCT05585801). Methods: LTx recipients were randomized to wearing an open-labelled or blinded CGM (Dexcom G6) added to standard care, placed in the infraclavicular region immediately after surgery. Data from the open-labelled CGM were used to adjust insulin therapy; blinded CGM data served as controls. Primary endpoint was &amp;gt; 7.5% difference in time in the target range 6-10 mmol/l. Results: We included 154 patients, 12 were excluded due to technical failures (6 from each arm), 142 patients were evaluated (active CGM n=82, control n=60), 28% with diabetes. All required vasopressors, mechanical ventilation and intravenous insulin therapy after transplantation. Patients with actively used CGM spent significantly more time in the target range compared to the control group (68.5% vs. 60.3%, p=0.003), meeting the primary endpoint, and significantly less time in level 1 hyperglycemia, see Table 1. Conclusion: Active use of CGM added to standard therapy significantly improved glycemic control in patients in critical state early after liver transplantation. Disclosure B. Hagerf (Voglová): None. M. Protus: None. L. Nemetova: None. M. Mraz: None. P. Girman: Speaker's Bureau; Neovii. M. Haluzik: Research Support; Sanofi. Advisory Panel; Eli Lilly and Company. Speaker's Bureau; Novo Nordisk, Abbott. Advisory Panel; AstraZeneca. Speaker's Bureau; GlaxoSmithKline plc, Amgen Inc. Advisory Panel; Bausch Health. J. Franekova: None. A. Jabor: None. E. Kieslichova: None. Funding CarDia (Programme EXCELES, Project No. LX22NPO5104); Funded by the European Union; Next Generation EUCooperatio Program, Charles University in Prague

The LVICE2 mission is dedicated to exploring the cislunar environment to address fundamental questions about solar wind fluctuations, plasma turbulence, and dust dynamics in Earth’s natural gravitational laboratories at the Moon’s Lagrangian points. By studying these pristine regions during solar maximum, the mission will support future space exploration and deepen understanding of astrophysical plasmas. In collaboration among Czech institutions, LVICE2 seeks to advance the Czech space industry’s capabilities while inspiring the next generation of scientists and engineers.

The Austro-Oriental shield-bug genus Axiagastus Dallas, 1851 (Hemiptera: Heteroptera: Pentatomoidea: Pentatomidae: Pentatominae: Axiagastini) is revised and found to be a senior synonym of Indrapura China, 1928, syn. nov. The following Axiagastus species (including five new) are treated: A. cambelli Distant, 1911 (= Pentatoma marmoratum Montrouzier, 1855, syn. nov.), from Australia (Queensland), Papua New Guinea, Solomon Islands, Fiji, Samoa and Vanuatu; A. chandrashekarai Salini, Kment & Roca-Cusachs sp. nov., from Indonesia (Sulawesi); A. dubius Jensen-Haarup, 1937 from ?Singapore; A. klossi (China, 1928, Indrapura), comb. nov., from Indonesia (Sumatra); A. luteipes Salini & Roca-Cusachs sp. nov. from Myanmar; A. mitescens Distant, 1901, from China; A. prathapani Salini & Kment sp. nov., from India (Andaman and Nicobar Islands), Indonesia (Java, Sulawesi, Sumatra), Laos, Malaysia (Malaya, Sabah, Sarawak), and Singapore; A. rosmarus Dallas, 1851 (type species) from the Philippines; A. votypkai Salini & Kment sp. nov., from Indonesia (Papua), Papua New Guinea, Australia (Queensland), and A. yeshwanthi Salini, Kment & Webb sp. nov., from Cambodia, India, Japan (Ryukyus), Taiwan, Thailand and Vietnam. Lectotypes are designated for A. cambelli, A. rosmarus and Pentatoma marmoratum. We maintain the prevailing usage of Axiagastus cambelli and an application to the International Commission on Zoological Nomenclature is prepared to suppress its senior but unused synonym Pentatoma marmoratum. A checklist and key to the included species of Axiagastus are provided. In addition, the species status of Acesines breviceps Stål, 1876 is discussed and the following new subjective synonymy is proposed: Acesines breviceps = Acesines bambusana Distant, 1918, syn. nov. Comments on the collections and type material of William S. Dallas, Francis Walker, Jean X. H. Montrouzier and the Macleay Museum (now part of Chau Chak Wing Museum, University of Sydney) are provided.

ABSTRACT Abandoned mines and other artificial roosts in Brisbane Forest Park (‘BFP’) were investigated between 2007-2009 and 2021-2023 to determine their potential as microbat roosts, particularly relating to the Eastern Horseshoe Bat Rhinolophus megaphyllus. Thirteen structures were identified as diurnal roosting sites used by R. megaphyllus, with the Little Bent-wing Bat Miniopterus australis and the Large Bent-wing Bat Miniopterus orianae oceanensis also found in one adit. Data derived from diurnal counts of roosting bats are presented here, as well as data on the depth and/or microclimate of structures. Specifically, adits were between 6 and 60 m in total depth with bats generally occupying the darkest portions. The maximum colony size noted during diurnal counts was 45 bats. A tunnel was also investigated for use by microbats in 2021-2023, with 89 R. megaphyllus counted during emergence. No maternity roosts were detected, although mating was observed incidentally in one adit in June. Trapping in late December in the two largest structures only recorded male R. megaphyllus, with one post-lactating and one juvenile female captured in January. The Brisbane area is generally devoid of cave-forming geology, so the population of R. megaphyllus in BFP is considered to be strongly dependent on these artificial underground features.

Cherax pulverulentus sp. nov. is a moderately-sized burrowing freshwater crayfish endemic to the streams west of the Ayamaru Lake in Bird's Head Peninsula (Framu Subdistrict, Southwest Papua Province), the Indonesian part of New Guinea. Although this species has been exploited in the ornamental aquarium trade at least for 21 years, it has not been formally named until now. Its commonly used commercial names in the pet trade are: "Hoa Creek", "Irian Jaya", or "Blue Moon" crayfish, but these names are also used for other more bluish or pinkish species of Cherax. Cherax pulverulentus sp. nov. is genetically and morphologically most similar to Cherax pulcher Lukhaup, 2015, but both species may be distinguished by several morphological characteristics and DNA sequence divergence, which support C. pulverulentus sp. nov. as a valid species.

We here update the distribution and provide a checklist of the water boatmen (Hemiptera: Heteroptera: Corixoidea: Corixidae) of Romania based on country-wide sampling, a literature survey, and private and museum collections. The Romanian fauna currently comprises 25 water boatmen species and subspecies in eight genera and two subfamilies. The occurrence of Glaenocorisa propinqua (Fieber, 1860), Arctocorisa carinata carinata (Sahlberg, 1819), and Corixa panzeri Fieber, 1848, all known from old and single records, is here confirmed. The first record of an intermediate shape of the pala between Sigara falleni (Fieber, 1848) (triangular) and S. iactans Jansson, 1983 (trapezoidal) is reported from the southeastern European population of the latter, suggesting possible hybridization.

Computed tomography (CT) is one of the most important imaging methods in clinical medical practice. Students of medicine have an opportunity to learn about its principles during the classes of the medical biophysics. For a high-quality understanding of the function of CT and to increase the curriculum attractiveness, it is advisable to include such clinical equipment or its simulator in the practical lessons. However, regarding operational safety and related maintenance, CT devices are very expensive and demanding. Therefore, the aim of this work was to create a cheap, operationally easy, and safe CT simulator. The device simulates the principle of CT by transmitting visible light through cubes with a built-in filter of defined attenuation simulating various human tissues. During hands-on training, students first measure radiation intensities without a sample (I0) and with single cubes (Ii). In the second task, students measure a matrix consisting of four different cubes, irradiating the matrix from different directions and simulating the CT principle. Based on the measurements, the students calculate the linear attenuation of each cube and the corresponding CT numbers and compare the results. The simulator's control is intuitive, provided measurement results are consistent and automatically fed into an interactive report that compares the measured data with the students' calculations, verifies the results, and finally stores them in the database. The main benefit of the study is to bring cheap and effective tools for the demonstration of the CT principle, which can operate each student during the practical lessons, followed by supporting teaching materials.