Pamukkale Üniversitesi Hastaneleri

Objective: Proteasome inhibitors and histone deacetylase (HDAC) inhibitors represent promising therapeutic agents in lung cancer, particularly by enhancing cancer cell sensitivity to treatment. Among these, the combination of ixazomib, a proteasome inhibitor, and givinostat, an HDAC inhibitor, has demonstrated synergistic cytotoxicity in non-small cell lung cancer (NSCLC) cell lines. This strategy holds potential for overcoming therapeutic resistance. Furthermore, macrophage co-culture systems have been shown to modulate gene expression patterns in lung cancer cells. In this context, the present study aimed to investigate the effects of ixazomib and givinostat, individually and in combination, along with differentiated M1 macrophages, on the expression of apoptosis-related genes in NSCLC cells. Material and Method: The IC50 values of ixazomib and givinostat in A549 lung cancer cells were determined using the MTT assay after 24 hours of treatment with a range of concentrations (400, 100, 10, and 1 µM). Based on the determined IC50 values, A549 cells were treated with ixazomib alone or in combination with givinostat for 24 hours. In parallel, A549 cells treated with the ixazomib–givinostat combination were co-cultured with THP-1 monocyte–derived M1 macrophages. Total RNA was then isolated, and RT-PCR analysis was performed to evaluate alterations in apoptosis-related gene expression. Result and Discussion: Based on the results of the anti-proliferative effect analysis, the IC50 values for ixazomib and givinostat were determined to be 2.42 µM and 7.32 µM, respectively. According to the RT-PCR results, variations of up to 48.99-fold were observed in the NFKB2, NFKBIA, NFKBIB, RELB, IL-6, IL-8, TP53, PIK3CA, and BCR genes. Significant alterations were particularly observed in genes involved in apoptosis-related pathways, especially in the presence of M1 macrophages. Additional investigation is warranted to thoroughly explore the heightened inflammatory response observed in lung cancer cells in the presence of these drugs.

Aim: Pediatric rheumatic diseases, such as Juvenile Idiopathic Arthritis (JIA), fibromyalgia, muscle strain, and scoliosis, can significantly impact physical function and quality of life. Exercise therapy is an effective, evidence-based approach for managing musculoskeletal symptoms in these patients, but its use in pediatric rheumatology remains underexplored. This study aimed to evaluate the prevalence of exercise prescription, the underlying clinical justifications, and demographic characteristics of pediatric rheumatology immigrant-background pediatric patients referred to a physical therapy clinic. Material and Method: This prospective, cross-sectional study included pediatric patients with immigrant (0-22 years) referred from the pediatric rheumatology clinic to the physical therapy clinic of Ümraniye Training and Research Hospital between January 1 and December 31, 2024. Patient data, including age, gender, diagnosis, and treatment type, were analyzed using SPSS (v.26), with p0.05). Muscle strain (74%) and joint pain (69%) had the highest exercise prescription rates, while JIA patients had a lower rate (47%). Conclusion: Exercise prescriptions are more common in non-inflammatory conditions like muscle strain and joint pain, while JIA patients receive them less frequently, likely due to concerns over joint damage and disease activity. Future studies should assess the long-term impact of exercise in this population.

Bu çalışmada, Denizli ili Tavas ilçesinde doğal olarak yetişen 38 badem genotipinin pomolojik ve kolorimetrik özellikleri incelenmiştir. İncelenen genotiplerde meyve ağırlığı 3,00-6,87 g, iç ağırlığı 0,26-1,98 g ve iç oranı %13,26-43,30 arasında değişim göstermiştir. Meyve boyutları açısından en yüksek varyasyon katsayısı uzunlukta (%18,70), en düşük ise kalınlıkta (%7,55) belirlenmiştir. Renk parametrelerinde L* değeri 36,51-55,90, a* değeri 10,11-15,23, b* değeri 26,16-39,93 arasında değişmiştir. Hiyerarşik kümeleme analizinde genotipler iki ana küme altında gruplandırılmış olup, genetik mesafe 1,21-10,05 arasında değişmiştir. Temel bileşenler analizinde (PCA) ilk beş bileşen toplam varyasyonun %68,84’ünü açıklamıştır. PC1 eksenine en yüksek katkıyı b* (0,433), meyve uzunluğu (0,389) ve iç uzunluğu (0,375) sağlarken, PC2 eksenine en yüksek katkıyı Chroma (0,429) ve iç genişliği (0,200) sağlamıştır. Çalışma sonucunda, incelenen popülasyonun önemli düzeyde genetik çeşitlilik gösterdiği ve özellikle 1, 5, 9 ve 17 numaralı genotiplerin ıslah çalışmaları için değerli genetik kaynaklar olabileceği belirlenmiştir.

Amaç:Bu araştirma pediatri hemşirelerinin rol ve fonksiyonlarini uygulama durumlarinin belirlenmesi amaciyla tanimlayici olarak yürütülmüştür

Veri madenciligi, ham verilerden cok net olmayan, onceden bilinmeyen fakat potansiyel olarak kullanisli bilginin elde edilmesidir. Bu da; kumeleme, veri ozetleme, degisikliklerin incelenmesi, sapmalarin tespiti gibi belirli sayida teknik yaklasimlari icerir. Veri madenciligi veri yiginlarindan, hedefe yonelik sorulara cevap verebilecek gereksiz verilerden temizlenmis cok degerli bilgilere ulasabilme yontemidir. Veri madenciligi ile ilgili genel bilgiler verilerek calismaya baslanmistir. Veri madenciligi ile pek cok alanda karar verme islemlerine yardimci olacak onemli sonuclar elde edilebilir. Cesitli yontemlerle veriler incelerek, ham verilerden anlamli verilere ulasilabilir. Veri madenciliginin yaygin bir uygulama alani vardir. Calismamiz, bu uygulama alanlarindan biri olan saglik sektorundeki verileri kapsamaktadir. PAU Hastane Bilgi Yonetim Sisteminde yer alan veriler kullanilarak gelen hastalarin profilini belirleyen bir uygulama calismasi yapilmistir. Uygulama ile verilen bilgilerin saglik profesyonellerinin en dogru ve guncel bilgiye ulasmasini, en objektif ve optimum cozumleri kullanmasini saglayacak bir karar destek araci oldugu gosterilmeye calisilmistir.

We describe marginal zone lymphoma of the mucosa-associated lymphoid tissue (MALT) and especially MALT lymphoma occurring in the conjunctiva. Tumors of the conjunctiva and cornea are grouped into two major categories of congenital and acquired lesions. Lymphoid tumors of the conjunctiva are acquired tumors and can occur as an isolated lesion or can be a manifestation of systemic lymphoma. Primary lymphomas of the conjunctiva are extremely rare usually originate from extranodal marginal zone B-cell non-Hodgkin's lymphomas of MALT and occur among older adults with a mean age of 61 years. In the last decade it has been reported that MALT lymphomas may develop in various extraintestinal locations, sometimes even without the presence of a mucosa. Half of MALT lymphomas occur in the gastrointestinal tract. MALT lymphomas of the eye are rare and originate from the conjunctiva and the lacrimal glands. Studies evaluating the clinical impact of 67Ga-C scintigraphy for MALT-type lymphomas imaging are rare, based on small numbers, heterogenous groups of patients. Clinical examination, excisional biopsy, histopathology and immunohistochemical studies, computerized tomography and magnetic resonance imaging are also used for the diagnosis of cunjunctival MALT disease. A case of ours gives reason for further discussion. Treatment and follow-up of MALT lymphoma is described.