Research Centre for Industrial Crops

The expected population rise will require a maximum exploitation of agricultural lands with a consequent increase in the demand for freshwater for irrigation uses. Future trends predict increasing periods of drought stress, which may impact on crop performance and limit the future production. Pepper is one of the most economically important crops and globally consumed vegetables. This crop is highly demanding in terms of water supply, and so far, developing tolerant cultivars is one of the main targets for breeding. The aim of this study is to accurately determine how pepper plants react to water stress at the vegetative stage in order to select genotypes that better cope with drought. We implemented the PhenoHort Plant Eye phenotyping platform to precisely assess changes in plant architecture and morpho-physiological parameters on 25 cultivated pepper genotypes (Capsicum annuum) under drought stress conditions. Three different irrigation supply levels were considered, including the control, intense, and severe water stress, by irrigating every 24, 72, and 96 h, respectively. Daily monitoring of 20 traits allowed ~190,000 multispectral and tridimensional data points through scans over 6 weeks of cultivation, thus shedding light on changes in plant architecture and vegetation indices’ values during stress. The dissection of genotype (G) and treatment (T) interactions revealed that digital biomass and plant height traits were strongly affected by the T factor (more than 50% of total variance), whereas color and multispectral parameters were under greater genotypic control, accounting for 58.27% and 64.97% of the total variance for HUE and NPCI, respectively. The comparison of each accession with respect to the control and the application of multivariate models allowed us to select four drought-tolerant lines (G1, G2, G22, and G25) able to reduce the effects of drought on the morphological parameters and architecture of the plant with positive effects on vegetative indices. This work represents the first attempt to dissect the response of pepper under drought stress at the vegetative stage using a high-throughput and non-invasive phenotyping system, offering new insights for selecting resilient genotypes.

Beekeeping is a crucial agricultural practice that significantly enhances environmental health and food production through effective pollination by honey bees. However, honey bees face numerous threats, including exotic parasites, large-scale transportation, and common agricultural practices that may increase the risk of parasite and pathogen transmission. A major threat is the Varroa destructor mite, which feeds on honey bee fat bodies and transmits viruses, leading to significant colony losses. Detecting the parasite and defining the intervention thresholds for effective treatment is a difficult and time-consuming task; different detection methods exist, but they are mainly based on human eye observations, resulting in low accuracy. This study introduces a digital portable scanner coupled with an AI algorithm (BeeVS) used to detect Varroa mites. The device works through image analysis of a sticky sheet previously placed under the beehive for some days, intercepting the Varroa mites that naturally fall. In this study, the scanner was tested for 17 weeks, receiving sheets from 5 beehives every week, and checking the accuracy, reliability, and speed of the method compared to conventional human visual inspection. The results highlighted the high repeatability of the measurements (R2 ≥ 0.998) and the high accuracy of the BeeVS device; when at least 10 mites per sheet were present, the device showed a cumulative percentage error below 1%, compared to approximately 20% for human visual observation. Given its repeatability and reliability, the device can be considered a valid tool for beekeepers and scientists, offering the opportunity to monitor many beehives in a short time, unlike visual counting, which is done on a sample basis.

In order to ensure food security worldwide in the face of current climatic changes, a higher quality and quantity of crops is necessary to sustain the growing human population. By developing a sustainable circular economy and biorefinery approaches, we can move from a petroleum-based to a bio-based economy. Plant biostimulants have long been considered an important source of plant growth stimulants in agronomy and agro-industries with both macroalgae (seaweeds) and microalgae (microalgae). There has been extensive exploration of macroalgae biostimulants. A lack of research and high production costs have constrained the commercial implementation of microalgal biostimulants, despite their positive impact on crop growth, development and yields. The current knowledge on potential biostimulatory compounds, key sources and their quantitative information from algae is summarized in the present review. Our goal is to provide a brief overview of the potential for microalgal biostimulants to improve crop production and quality. A number of key aspects are discussed, including the biostimulant effects caused by microalgae extracts, as well as the feasibility and potential for cocultures and coapplication with other biostimulants and biofertilizers. This article also discusses the current knowledge, recent developments and achievements in extraction techniques, types of applications, timings of applications. Ultimately, this review highlights the potential for microalgal biostimulants for sustainable agricultural practices, the algal biochemical components contributing to these traits, and finally bottlenecks and involved prospects in commercializing microalgal biostimulants.

<p>Cacti are icons of our time. Symbols of the 21st century lifestyle, they populate the windowsills of city flats and family villas, decorate shop windows and office desks. Each species has a characteristic that catches the eye: the colour, the bizarre shape, the thorns, the fascinating flowers. As the name suggests, succulents store water in their tissues, roots and thick, fleshy stems. Often counted apart, cacti are a type of succulent that has adapted to survive in the most inhospitable conditions on Earth. Their reputation as 'die-hards' makes these resilient plants easy to handle even for the novice. This review describes the characteristics and cultivation methods of one of the most interesting cacti, the <em>Melocactus</em>.</p>

Contrary to the biosynthetic pathways of many terpenoids, which are well characterized and elucidated, their transport inside subcellular compartments and the secretion of reaction intermediates and final products at the short- (cell-to-cell), medium- (tissue-to-tissue), and long-distance (organ-to-organ) levels are still poorly understood, with some limited exceptions. In this review, we aim to describe the state of the art of the transport of several terpene classes that have important physiological and ecological roles or that represent high-value bioactive molecules. Among the tens of thousands of terpenoids identified in the plant kingdom, only less than 20 have been characterized from the point of view of their transport and localization. Most terpenoids are secreted in the apoplast or stored in the vacuoles by the action of ATP-binding cassette (ABC) transporters. However, little information is available regarding the movement of terpenoid biosynthetic intermediates from plastids and the endoplasmic reticulum to the cytosol. Through a description of the transport mechanisms of cytosol- or plastid-synthesized terpenes, we attempt to provide some hypotheses, suggestions, and general schemes about the trafficking of different substrates, intermediates, and final products, which might help develop novel strategies and approaches to allow for the future identification of terpenoid transporters that are still uncharacterized.

Simple SummaryHemp hurd is the inner bark of the plant’s stem and is typically seen as waste. The objective of this experiment was to describe the physical characteristics, such as moisture, water absorption, and ammonia absorption of ten hemp varieties (Fibranova, Codimono, USO31, CS, Futura 75, Eletta Campana, Carmaleonte, Felina 32, Santhica, and Ferimon) cultivated in Italy to be used for livestock bedding. The samples were hemp shives, obtained from hemp hurds ground to 8 mm. The results showed an average of 7.78%, 251.9%, and 50.0% for moisture, water absorption, and ammonia absorption, respectively. Moisture and water absorption were similar among varieties. A significant difference between varieties was found for the ammonia absorption, varying from 45.0 to 55.5% for the Fibranova and Ferimon, respectively. In conclusion, hemp shives have good physical characteristics, similar to other commercial bedding materials (i.e., wood shavings) but other parameters and on-farm trials will be required to make a full assessment of hemp.Hemp (Cannabis sativa L.) hurds, the inner bark of the stem, are a poorly appreciated part of the plant that typically represents waste. The aim of this experiment was to describe the physical characteristics, including moisture (M), water absorption (WA), and ammonia absorption (AA), of 10 hemp varieties (Fibranova, Codimono, USO31, CS, Futura 75, Eletta Campana, Carmaleonte, Felina 32, Santhica, and Ferimon) cultivated in Italy. Samples of hemp hurds were ground to 8 mm obtaining hemp shives. Values of M, WA, and AA were determined following the official procedures. The results showed an average of 7.78%, 251.9%, and 50.0% for M, WA, and AA, respectively. Data of M and WA were similar among varieties, whereas a significant difference was found for the AA, varying from 45.0 to 55.5% for the Fibranova and Ferimon varieties, respectively. In conclusion, hemp shives have good physical characteristics, similar to other commercial bedding materials (i.e., wood shavings) but other parameters and on-farm trials will be required to make a full assessment of hemp.

Plant tolerance to salinity stress is vital for irrigation scheduling, decision-making, planning and operation, and water resource management. This study is aimed to investigate the effects of irrigation water salinity on water use, growth, physiology, and yield parameters of drip-irrigated tomatoes in two different growing seasons. In addition to control (0.7 dS m−1), three irrigation water salinity levels: 2.5 (low), 5.0 (moderate), and 7.5 (high) dS m−1 were used. In both seasons, autumn 2021 and spring 2022, increased water salinities caused an increase in soil salinity, while a decrease in seasonal crop evapotranspiration. Plant heights did not show significant differences under different salinity levels in autumn 2021, while a meaningful difference among treatments was found in spring 2022. Marketable and total tomato yields, and the leaf area index showed significant decreases under increased salinities in both seasons. Stomatal conductance was not affected by salinity levels. The salt tolerance models for marketable and total fruit yields showed a low threshold and slope value in autumn 2021, contrasting with a high threshold and slope value determined in spring 2022. The results suggest that the salinity slope value should be considered, as well as the salinity threshold value, for decision making in tomato production at different growing seasons.

Eruca sativa Miller (Brassicaceae) is an insect-pollinated diploid annual species which grows spontaneously in the entire Mediterranean basin from semi-arid to arid-hot conditions and is cultivated in Northern America, Europe, and Asia as either salad or oilseed crop. Here, some essential background was provided on this versatile crop, summarizing the present status of Eruca sativa research focusing on the wealth of bioactive ingredients in its seeds, which may find exploitation in agriculture, in the food industries and as nutraceuticals for their antioxidant and anti-inflammatory properties. Fatty acids of Eruca sativa seed oil, gums, glucosinolates and soluble and insoluble phenol and flavonoid fractions in the defatted press cake are the main bioactive compounds considered to date by the scientific literature and that deserve attention for their physical and biological activities.

There is growing evidence that pesticides may be among the causes of worldwide bee declines, which has resulted in repeated calls for their increased scrutiny in regulatory assessments. One recurring concern is that the current frameworks may be biased towards assessing risks to the honey bee. This paradigm requires extrapolating toxicity information across bee species. Most research effort has therefore focused on quantifying differences in sensitivity across species. However, our understanding of how responses to pesticides may vary within a species is still very poor. Here we take the first steps towards filling this knowledge gap by comparing acute, lethal hazards in sexes and castes of the eusocial bee Bombus terrestris and in sexes of the solitary bee Osmia bicornis after oral and contact exposure to the pesticides sulfoxaflor, Amistar (azoxystrobin) and glyphosate. We show that sensitivity towards pesticides varies significantly both within and across species. Bee weight was a meaningful predictor of pesticide susceptibility. However, weight could not fully explain the observed differences, which suggests the existence of unexplored mechanisms regulating pesticide sensitivity across bee sexes and castes. Our data show that intra-specific responses are an overlooked yet important aspect of the risk assessment of pesticides in bees.

<strong>Research objective:</strong> The objective of this work was to evaluate the properties and effect of certain organic fertilizers that can be used in organic farming in comparison with fertilizers of inorganic origin on <em>Abelmoschus esculenthus</em> plants. In addition, the interactions that can develop with microorganisms in the rhizosphere and the possible beneficial effects on transplant stress, nutrient uptake by roots and reduction of plant mortality were investigated. <strong>Materials and Methods:</strong> The experiments, which began in May 2022, were conducted in the greenhouses of CREA-OF in Pescia on plants placed in pots 12 cm in diameter and on which fertilizers of organic and inorganic origin were evaluated. In September 2022, plant height, number of leaves, leaf surface area, vegetative weight, root volume, root length, seeds per plant, number, weight, girth and length of pods, total substrate bacteria, substrate pH and number of dead plants were analysed. <strong>Results and Discussion:</strong> The experiment showed that the application of organic nitrogen and potassium fertilizers can significantly improve the quality and yield of <em>Abelmoschus esculenthus</em> plants. In general, a significant increase in height and vegetative and root growth of the plants was observed, especially in nitrogen and potassium synthesis, with an improvement also in the number of fruits and seeds per plant. Plant mortality also decreased significantly compared to the control with synthetic fertilizers, and microbial colonisation of the substrate increased, which probably affected the plants by influencing both vegetative and root growth and defence against biotic stressors. <strong>Conclusions:</strong> Organic fertiliser trials showed that these products can significantly improve plant growth by positively affecting the soil microbial component. This is an additional opportunity, especially for those farmers who not only seek to reduce the use of synthetic fertilizers, but also want to use sustainable techniques that better preserve plants and soils, while guaranteeing the production of quality products.