Resource Conservation District of Santa Cruz County

Abstract Environmental DNA (eDNA) detections of imperiled species have the potential to inform conservation action, but this requires the acceptance of new technologies by decision‐makers. Here we describe how engaging stakeholders into a collaborative process led to the successful translation of new eDNA findings into conservation outcomes. We characterized the distribution of three federally listed pond‐breeding amphibians across nearly 200 wetlands in coastal California using both traditional field surveys and eDNA sampling; the latter had greater detection rates overall. Regulatory agency staff gained trust in the rigor and effectiveness of eDNA data by joining traditional surveys and through the collaborative development of recommendations for the adoption of eDNA methods. Extensive outreach to the local community within the range of the highly endangered Santa Cruz long‐toed salamander resulted in invitations to sample previously unsurveyed wetlands on private property and the detection of new breeding sites. Conservation organizations and resource management agencies were integrated into our core team from the start, and ultimately shaped wetland management actions, siting of new wetlands, and land acquisition priorities informed by the data generated. Thus, this project serves as a model for actionable eDNA science directly affecting conservation.