Rongsheng Petrochemical (China)

The Epstein-Barr virus (EBV) is associated with multiple lymphoid malignancies and autoimmune diseases. The glycoprotein gp350, the most abundant envelope protein on the EBV virion surface, has been the primary target for the vaccine design. However, all the attempts have failed to prevent asymptomatic infection in clinical trials. This setback highlights an incomplete understanding of the immune response to gp350 and emphasizes the need for more comprehensive antibody studies. Here, we identified three human-derived anti-gp350 monoclonal antibodies from a nonimmune human scFv library. Of these, the S54 antibody exhibited a potent neutralizing activity against virus infection of Raji B cells. Using cryo-EM, we resolved the structure of the S54-gp350 complex, revealing that the S54 epitope substantially overlaps with the CR2 recognition site. Our findings elucidate the molecular mechanism by which anti-gp350 antibodies block EBV infection of B cells in vitro, providing valuable insights for antibody-based therapy and vaccine development.

Current therapeutics for chronic hepatitis B virus (HBV) infection are insufficient due to immune exhaustion caused by high circulating hepatitis B surface antigen (HBsAg) levels. Here, an HBV S region-targeted small interfering RNA (siRNA) is reported, aiming to dramatically suppress HBsAg and provide windows for host immune restoration. This siRNA agent, called KC13-M2G2, exhibits potent antiviral efficacy against all HBV genotypes in vitro. Notably, in multiple mouse models, KC13-M2G2 triggers rapid and sustained loss of HBsAg and HBV DNA accompanied by hepatitis B surface antibody seroconversion, outperforming the clinical drug elebsiran and bepirovirsen. Toxicity studies in Sprague-Dawley rats and cynomolgus monkeys indicate satisfactory biosafety profiles of KC13-M2G2. Given that elebsiran and bepirovirsen have achieved a functional cure rate of no more than 20% in their clinical studies, KC13-M2G2 as a more potent candidate drug is expected to exhibit superior performance in clinical applications.

Surface plasmon resonance has been generally employed to enhance the photoresponse in typical fields such as energy conversion and chemical sensing. In this study, we propose a plasmon resonance enhanced polishing (PREP) method based on in-process synthesized Ag/AgCl/g-C3N4 ternary heterojunctions, which overcomes three critical bottlenecks in conventional photochemical mechanical polishing including complex ex-process synthesis protocols, short-wavelength-limited photoresponse, and rapid charge carrier recombination. By synergistically integrating localized surface plasmon resonance (LSPR) from Ag nanoparticles with a Z-scheme charge transfer mechanism in the AgCl/g-C3N4 heterojunction, we achieve an expanded light absorption range up to 625 nm and significantly improve the separation efficiency of photogenerated carriers. Experimental results demonstrate that the in-process synthesized heterojunction photocatalysts facilitate a 3.02-fold increase in material removal rate under broad-spectrum solar illumination while maintaining close-to-atomic-level surface roughness (0.37 nm). This work not only provides fundamental insights into the dynamic construction of heterojunction architectures but also opens up new application scenarios of the heterojunction-based plasmon resonance phenomenon.

ObjectiveTo evaluate and compare the safety and efficacy of two treatments for cervical spondylosis. One treatment involves the use of electronic wrist–ankle acupuncture, in combination with Tuina, while the other treatment involves using traditional wrist–ankle acupuncture, along with Tuina. The objective was to provide evidence‐based support for the treatment of cervical spondylosis using innovative medical device E‐WAA combined with Tuina and provide a better treatment option for patients with cervical spondylosis.MethodA total of 164 patients with cervical spondylosis from June 2021 to December 2022 were randomly assigned to a control group and an experimental group. The control group received traditional wrist–ankle acupuncture combined with Tuina, while the experimental group received the electronic wrist–ankle acupuncture combined with Tuina. Both groups underwent treatment twice a week for a total of 3 weeks. The primary outcome measure was the improvement rate of visual analog scale score, while the secondary outcome measures were the Neck Disability Index score and cervical range of motion. Safety measures included the recording of adverse reactions during treatment and subjective evaluation of the treatment methods by the patients. Clinical data were analyzed from January to March 2023.ResultBoth treatment modalities demonstrated improvements in pain and cervical function. There was no significant difference in the VAS improvement rate between the two groups (p = 0.593). However, the electronic wrist–ankle acupuncture combined with Tuina showed an additional benefit in reducing the NDI scores compared to the control group (p = 0.017). During the study, no adverse reaction was reported, and 83.75% of patients considered the electronic wrist–ankle acupuncture device to be safe.ConclusionBoth the electronic wrist–ankle acupuncture combined with Tuina and the traditional wrist–ankle acupuncture combined with Tuina showed significant therapeutic effects on cervical spondylosis. In comparison with the traditional wrist–ankle acupuncture, the electronic wrist–ankle acupuncture offers advantages such as noninvasiveness, controllability, and autonomous operation.SignificanceThese findings suggest that the electronic wrist–ankle acupuncture can serve as an adjunctive therapeutic approach for patients with cervical spondylosis, providing a more convenient treatment option.Trial RegistrationClinical Trial Registry: ChiCTR2200056394.

The disposal of postconsumer textiles has a significant impact on environmental sustainability. Herein, we have developed a spinnable poly(ethylene terephthalate) (PET) based copolyester, poly(ethylene terephthalate-co-glycolate) (PET-GA), which possesses both good usability and biodegradability for fabrics. The copolymerizations of ethylene glycol, terephthalic acid, and glycolic acid catalyzed with stannous octanoate, tetrabutyl titanate (TBT), ethylene glycol antimony, and antimony trioxide were investigated. The copolyester chain structures and their thermal property, rheological performance, and spinnability were characterized, and the mechanical properties and degradability of the copolyester fibers made by a melt spinning together with a post-stretching were evaluated. We found that TBT at 0.004 mol % of the total acid group was suitable for synthesizing PET-GAs with good usability and degradability. The filament yarns exhibited excellent performance with breaking strength up to 3.70 cN/dtex and dyeability by using a conventional polyester dyeing process. In addition, enhanced degradability was observed in both an alkali hydrolysis and a composting experiment. The PET-GA fibers degraded 15.8 times faster than PET fibers, while 40.4 wt % of the PET-GA fibers was biodecomposed by microorganisms in 140 days. Such copolyester fibers are believed to be beneficial for their recycling, making them a promising solution for the textile waste problem.

With the improvement of people s living standard and the progress of medical science, the application of blood products in clinical treatment and disease prevention and control has become more and more extensive and in greater demand. The most important raw material for the production of blood products is healthy human plasma, and the plasma must be collected by the single plasma collection station set up by blood product manufacturers to recruit healthy people (called plasma donors) to voluntarily donate plasma, due to the completely voluntary nature of plasma donors plasma donations, their individual behaviors are susceptible to the influence of a variety of factors, and the need for plasma donors team development and stabilization has posed a serious challenge to single plasma collection stations. This paper briefly dis- cusses the development and stability of the plasma donor team in the single plasma station, hoping to play a guiding role in the development of the blood source of the single plasma industry.