Sema4 (United States)

The action to stop operational activities of coal mining production at PT Semesta Alam Barito often occurred in 2023, resulting in the company's loss in the form of coal production loss. This incident is a picture of a less harmonious face between the company and the community around the mine. The company's presence and communication seem to lack synergy. This research was conducted to show good and correct practices regarding the Community Empowerment Program, which is one of the government's mandates in implementing GMP (Good Mining Practice) principles, implementing ESG (Environmental, Social, and Governance), implementing SDGs (Sustainable Development Goals), and also implementing the appeals of Murung Raya Regency government at Central Kalimantan. The research was conducted using descriptive quantitative and qualitative methods through literature review and direct observation in the field. The author applies improvement in the concept of the Community Empowerment Program and concludes optimistically that the right and competent personnel runs the Community Empowerment Program, carried out with a sincere heart, and implemented consistently and with full commitment, then portal activities that result in production loss can be suppressed, synergy of community with the company can be achieved, and an independent and developing community can be created.

The postpartum period is a crisis situation for mothers due to various changes that occur physically, psychologically, and family structures that require an adaptation or adjustment process. From the records of the Jepara District Health Office in 2021, data obtained from 127 of 392 maternity mothers (32.4%) experienced postpartum blues symptoms between the ages of 17-19 years. This study aims to determine the relationship of adolescent postpartum mothers to the incidence of post partum blues in the District of Nalumsari Jepara. This study uses an analytical design with a cross sectional approach. The population in this study were mothers who gave birth to teenagers in July 2022 at the Nalumsari Health Center Jepara with a total of 35 respondents. The sampling technique used was total sampling. The research instrument used the EPDS questionnaire. Data analysis was performed using the Chi-Square statistical test. The results showed that (1) most of the respondents as many as 20 people (57.1%) were in their late teens (16-19). (2) The incidence of Post partum Blues in postpartum mothers who did not experience postpartum blues were 14 people (40%), mild postpartum blues were 9 people (25.7%) and severe postpartum blues were 12 people (34.3%) . (3) the results of the bivariate analysis showed that there was no relationship between postpartum mothers in their teens to the incidence of post partum blues in the District of Nalumsari Jepara (p-value 0.254 > 0.05). This shows that age is not the main factor in the incidence of postpartum blues but is caused by other factors such as support from husbands, parents and physical fatigue. In addition, the level of maturity of a person cannot be determined by age alone. It is expected that mothers who will undergo childbirth will increase their knowledge regarding psychological adaptation during the postpartum period and the factors that influence the incidence of postpartum blues by carrying out activities during antenatal care so that there is preparation to undergo the delivery process until postpartum and take good care of the baby.

To determine the influence of plant protection products and fertilizers on the yield and quality of sunflower hybrid NK Kondi seeds. Methods. The research was conducted at the SFH “Golden Ear” in the Chortkiv district of the Ternopil region. The study examined the mid-ripening sunflower hybrid NK Kondi and 3 schemes of application of plant protection products, fertilizers, and growth stimulants: 1) Verno FG (0.5 kg/ha) + Nordox 75 WG (0.3 kg/ha); 2) Verno FG (0.5 kg/ha), Mira, RK (0.75 l/ha) + Nordox 75WG (0.3 kg/ha), Mira, RK (0.75 l/ha); 3) Nordox 75WG (0.3 kg/ha) + Nordox 75WG (0.3 kg/ha). The products were applied twice, at the 4–6 leaf stage and at the bud stage. Control – standard cultivation technology, including the application of plant protection products (fungicides and insecticides) and plant growth regulator. Results. The study showed that the highest quality and yield of sunflower seeds were found in the variant with the twice application of the contact fungicide Nordox 75. The lowest yield was recorded in the control variant – 2.9 t/ha, and the highest in the variant with the application of the contact fungicide Nordox 75, with a consumption rate of 0.3 kg/ha – 3.94 t/ha. The oil content in the variants using micro-fertilizer Verno FG, contact fungicide Nordox 75, and growth stimulator Mira RK ranged from 52.0 to 53.6%, corresponding to class 1 of sunflower seed quality according to DSTU 7011:2009. It is worth noting that the twice application of the contact fungicide Nordox 75 in the 4–6 leaf and bud stages contributed to a 9% increase in oil yield compared to the control variant. The quality indicator of sunflower seeds - the acid number of the oil in all study variants varied from 4.1 to 4.8 mg KOH/g. The weight of 1000 seeds also depended on the cultivation variant. With the twice application of the contact fungicide Nordox 75, seeds with the highest weight of 1000 seeds were obtained – 71.9 g, and the control had the lowest – 66.8 g. The germination energy of the investigated sunflower seeds ranged from 94 to 96%, and similarity ranged from 95 to 97%. Conclusions. The research results justify the conclusion that high yield and quality of sunflower seeds can be achieved through intensive cultivation technologies, which include the use of chemical plant protection products, fertilizers, and plant growth stimulants. The obtained data regarding the yield level and quality indicators of sunflower hybrid NK Kondi seeds indicate the prospects of its cultivation in the Western Forest-Steppe zone of Ukraine.

Emerging evidence suggests that cryptic translation beyond the annotated translatome produces proteins with developmental or physiological functions. However, functions of cryptic non-canonical open reading frames (ORFs) in cancer remain largely unknown. To fill this gap and systematically identify colorectal cancer (CRC) dependency on non-canonical ORFs, we apply an integrative multiomic strategy, combining ribosome profiling and a CRISPR–Cas9 knockout screen with large-scale analysis of molecular and clinical data. Many such ORFs are upregulated in CRC compared to normal tissues and are associated with clinically relevant molecular subtypes. We confirm the in vivo tumor-promoting function of the microprotein SMIMP, encoded by a primate-specific, long noncoding RNA, the expression of which is associated with poor prognosis in CRC, is low in normal tissues and is specifically elevated in CRC and several other cancer types. Mechanistically, SMIMP interacts with the ATPase-forming domains of SMC1A, the core subunit of the cohesin complex, and facilitates SMC1A binding to cis-regulatory elements to promote epigenetic repression of the tumor-suppressive cell cycle regulators encoded by CDKN1A and CDKN2B. Thus, our study reveals a cryptic microprotein as an important component of cohesin-mediated gene regulation and suggests that the ‘dark’ proteome, encoded by cryptic non-canonical ORFs, may contain potential therapeutic or diagnostic targets.

ObjectivesTo define pregnancy episodes and estimate gestational age within electronic health record (EHR) data from the National COVID Cohort Collaborative (N3C).Materials and MethodsWe developed a comprehensive approach, named Hierarchy and rule-based pregnancy episode Inference integrated with Pregnancy Progression Signatures (HIPPS), and applied it to EHR data in the N3C (January 1, 2018–April 7, 2022). HIPPS combines: (1) an extension of a previously published pregnancy episode algorithm, (2) a novel algorithm to detect gestational age-specific signatures of a progressing pregnancy for further episode support, and (3) pregnancy start date inference. Clinicians performed validation of HIPPS on a subset of episodes. We then generated pregnancy cohorts based on gestational age precision and pregnancy outcomes for assessment of accuracy and comparison of COVID-19 and other characteristics.ResultsWe identified 628 165 pregnant persons with 816 471 pregnancy episodes, of which 52.3% were live births, 24.4% were other outcomes (stillbirth, ectopic pregnancy, abortions), and 23.3% had unknown outcomes. Clinician validation agreed 98.8% with HIPPS-identified episodes. We were able to estimate start dates within 1 week of precision for 475 433 (58.2%) episodes. 62 540 (7.7%) episodes had incident COVID-19 during pregnancy.DiscussionHIPPS provides measures of support for pregnancy-related variables such as gestational age and pregnancy outcomes based on N3C data. Gestational age precision allows researchers to find time to events with reasonable confidence.ConclusionWe have developed a novel and robust approach for inferring pregnancy episodes and gestational age that addresses data inconsistency and missingness in EHR data.

Adoption of genome sequencing (GS) as a first-line test requires evaluation of its diagnostic yield. We evaluated the GS and targeted gene panel (TGP) testing in diverse pediatric patients (probands) with suspected genetic conditions. Probands with neurologic, cardiac, or immunologic conditions were offered GS and TGP testing. Diagnostic yield was compared using a fully paired study design. 645 probands (median age 9 years) underwent genetic testing, and 113 (17.5%) received a molecular diagnosis. Among 642 probands with both GS and TGP testing, GS yielded 106 (16.5%) and TGPs yielded 52 (8.1%) diagnoses ( P < .001). Yield was greater for GS vs . TGPs in Hispanic/Latino(a) (17.2% vs . 9.5%, P < .001) and White/European American (19.8% vs . 7.9%, P < .001), but not in Black/African American (11.5% vs . 7.7%, P = .22) population groups by self-report. A higher rate of inconclusive results was seen in the Black/African American (63.8%) vs . White/European American (47.6%; P = .01) population group. Most causal copy number variants (17 of 19) and mosaic variants (6 of 8) were detected only by GS. GS may yield up to twice as many diagnoses in pediatric patients compared to TGP testing, but not yet across all population groups.

Abstract Background: The 21-gene Recurrence Score (Oncotype DX) is a genomic assay that provides prognostic information for distant recurrence risk and is predictive of adjuvant chemotherapy benefit in hormone receptor (HR)-positive, HER-2 negative early-stage breast cancer (EBC). The Breast Cancer Index (BCI) is another molecular gene expression-based assay that evaluates the utility of extending adjuvant endocrine therapy (ET) from 5 to 10 years and predicts risk of distant recurrence. In January 2021, the National Comprehensive Cancer Network (NCCN) Guidelines added BCI to guide duration of adjuvant ET as a category 2A recommendation. The goal of this study was to evaluate the association between BCI and RS in terms of their predicted benefit for adjuvant therapy and risk of distant recurrence. We also assessed the association of various anatomic and biologic tumor features with BCI. Methods: We performed a retrospective chart review of all patients with HR-positive EBC who had a BCI and Oncotype DX performed between 2007-2021. Demographics, tumor characteristics and BCI and RS results were extracted from the electronic medical record. Patients were categorized by BCI predictive of extended ET (formerly BCI high) versus not (formerly BCI low) and RS of low (0-10), intermediate (11-25) and high (26-100). Numerical values for distant recurrence risk were recorded for both BCI and Oncotype DX tests. Multivariable regression models were used to assess the relationship between BCI and Oncotype DX as well as factors associated with each. Results: We identified 153 women with HR-positive EBC with both RS and BCI performed. The median age of the population was 57 years and 25% were premenopausal. 32% (n=49) had a BCI result predictive of benefit from extended adjuvant ET. When comparing patients with BCI predictive of extended ET versus those with BCI not predictive of extended ET, there was no association between BCI and RS based on multivariate logistic regression models, p=0.7. A similar distribution of RS was observed between patients who had a BCI result predictive of benefit from extended ET versus not predictive. Among 49 patients with a BCI predictive of extended ET, 35% had high RS, 63% intermediate RS and 2% low RS. Among 104 patients with a BCI not predictive of extended ET, 24%, 73% and 3% had high, intermediate, and low RS, respectively. Multivariate regression models revealed an association between poorly differentiated tumors and BCI result predictive of extended ET, p=0.002. No associations were observed between BCI and menopausal status, ER%, PR%, tumor size or lymph node positivity. Regarding risk of recurrence, there was an association between BCI and Oncotype DX in terms of their predicted numerical risk of recurrence, p&amp;lt; 0.001. Higher percentage of PR positivity, poorly differentiated tumors, and lymph node positivity were associated with a higher risk of recurrence on the BCI. Conclusions: In our patient population selected to have Oncotype DX and BCI performed, we found no association between the two genomic assays in terms of their predictive benefit. However, there was an association between Oncotype DX and BCI in terms of their prognostic ability. Given the increased use of BCI since its inclusion in national guidelines, it is important to understand its relationship with other genomic assays especially when used to guide clinical decisions and estimate prognosis. Citation Format: Rima Patel, Nicole Casasanta, Zhiqiang Li, Melanie Kier, Julia Blanter, Sophie Sohval, Malin Hovstadius, Catherine Wu, Marc Fink, Xiang Zhou, Brittney Zimmerman, Krystal Cascetta, Rong Chen, William Oh, Amy Tiersten. Correlating Predicted Adjuvant Therapy Benefit and Risk of Recurrence between Breast Cancer Index (BCI) and 21-gene Oncotype DX Recurrence Score (RS) [abstract]. In: Proceedings of the 2022 San Antonio Breast Cancer Symposium; 2022 Dec 6-10; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2023;83(5 Suppl):Abstract nr P1-04-09.

The optimal fractionation schedule in unresected stage I non-small cell lung cancer (NSCLC) unsuitable for stereotactic body radiation therapy is unclear. Given the lack of comparative data regarding nonstereotactic body radiation therapy schemas, we compared overall survival (OS) with hypofractionated radiotherapy (HFRT) versus conventionally fractionated radiotherapy (CFRT) and examined the OS impact of different HFRT doses. This retrospective analysis included 2159 patients from the National Cancer Database diagnosed with stage I (cT1-2aN0M0) NSCLC between 2008 and 2016. Patients underwent CFRT (70≤BED10 [biologically effective dose] <100 Gy10 in ≥30 fractions), low-dose HFRT (LD-HFRT; 70≤BED10 [assuming α/β=10] <100 Gy10 in 11 to 24 fractions), or high-dose HFRT (HD-HFRT; 100≤BED10 ≤120 Gy10 in 6 to 10 fractions). Patients who received surgery, chemotherapy, or immunotherapy were excluded. We compared CFRT versus all HFRT, and separately CFRT versus LD-HFRT and CFRT versus HD-HFRT. OS was evaluated with the Kaplan-Meier estimator, log-rank test, and Cox regression. A total of 63.2% of patients underwent CFRT, 23.5% LD-HFRT, and 13.3% HD-HFRT. OS was significantly longer with HFRT versus CFRT on univariable (28.2 mo [95% CI, 25.6-31.7] vs 26.4 mo [25.0-27.9]; log-rank=0.0025) but not multivariable analysis (MVA; hazard ratio [HR] 0.90; P=0.062). MVA yielded no significant difference in OS between CFRT and LD-HFRT (HR 0.96, P=0.53). OS was significantly longer with HD-HFRT versus CFRT on MVA (HR, 0.75; P=0.003). However, on sensitivity analysis using different multivariable modeling techniques, this did not retain statistical significance (HR, 0.83; P=0.12). For stage I NSCLC, HFRT does not show a robust OS benefit compared with CFRT but may be preferred given the convenience and lower costs.