South Dakota Department Of Education

Identifying pathogens in complex samples such as blood, urine, and wastewater is critical to detect infection and inform optimal treatment. Surface-enhanced Raman spectroscopy (SERS) and machine learning (ML) can distinguish among multiple pathogen species, but processing complex fluid samples to sensitively and specifically detect pathogens remains an outstanding challenge. Here, we develop an acoustic bioprinter to digitize samples into millions of droplets, each containing just a few cells, which are identified with SERS and ML. We demonstrate rapid printing of 2 pL droplets from solutions containing S. epidermidis, E. coli, and blood; when mixed with gold nanorods (GNRs), SERS enhancements of up to 1500x are achieved.We then train a ML model and achieve >=99% classification accuracy from cellularly-pure samples, and >=87% accuracy from cellularly-mixed samples. We also obtain >=90% accuracy from droplets with pathogen:blood cell ratios <1. Our combined bioprinting and SERS platform could accelerate rapid, sensitive pathogen detection in clinical, environmental, and industrial settings.

Pierre Michel: What were the working methods during rehearsals at the time of the first album?Pierre Michel : Quelles étaient vos méthodes de travail pendant les répétitions à l’époque du premier album de Hatfield and the North ? Dave Stewart: Richard wrote most of his material on guitar, Phil wrote exclusively on guitar, Pip’s pieces on the first album were written on guitar (he later used piano) and all my music was written on piano. Before the four of us got together for band rehearsals, ...

Nous avions présenté quelques aspects du déroulement matériel d’une naissance au Moyen Âge lors du séminaire de Questes. L’intérêt de nos collègues pour ces realia nous a poussée à les présenter ici, les enrichissant de références bibliographiques. Pour mieux percevoir cet événement qui relève de la sphère intime et qui est donc fort peu documenté, nous disposons de quatre sortes de sources. Deux sont normatives : les traités de médecine et les manuels d’éducation. Les deux autres nous livren...

This study was inspired by the final regulations for the No Child Left Behind Act (NCLB) indicating that each state has the option to develop a new assessment for students whose disabilities have kept them from obtaining proficiency. Sets of high school science achievement items were enhanced for the new test. A 3-by-2, within subjects, experimental design was used with 400 participants from 3 states. Although items remained connected to grade-level content standards, in the enhanced condition they were easier for all groups. Changes in measurement precision were mixed for students in the group eligible for the new examination. Participants tended to report that individual enhancements made items easier, but that packages of enhancements did not affect the difficulty of items.

In South Dakota, chronic wasting disease (CWD) was first discovered during the winter of 1997–1998 in seven privately owned captive elk herds. This prompted the South Dakota Department of Game, Fis...

Journal Article Badgers Feed on Rattlesnakes Get access A. M. Jackley A. M. Jackley Pierre, South Dakota Search for other works by this author on: Oxford Academic Google Scholar Journal of Mammalogy, Volume 19, Issue 3, 18 August 1938, Pages 374–375, https://doi.org/10.1093/jmammal/19.3.374-a Published: 18 August 1938

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