Telesis Corporation (United States)

Delivery of biomolecules into plant vascular tissues remains a barrier to managing diseases caused by insect vector-borne pathogens and to modifying phenotypes of established perennial crops. Inspired by the vascularized growth of crown galls induced by Agrobacterium tumefaciens, we repurposed the bacterium’s plant growth regulator (PGR) genes to engineer autonomously dividing, transgene-expressing plant cell structures termed symbionts. A plant transformation vector (pSYM) incorporating the IaaM, IaaH, Ipt and gene5 cassette from A. tumefaciens strain C58 together with a gene of interest on the same transfer DNA was delivered to stems of herbaceous and woody dicots using disarmed A. tumefaciens strain EHA105. Symbiont morphology, vascular differentiation, transgene expression, molecular mobility and protein secretion were evaluated using microscopy, fluorescent reporters, dye tracing, RNA silencing assays and mass spectrometry-based proteomics. pSym inoculation reproducibly generated symbionts across diverse host plant species that were vascularly integrated into their host plants and transgene expression ranging from heterogeneous niches to more uniform patterns. Small molecules moved between symbionts and host vascular tissues, whereas larger proteins exhibited more restricted mobility. Post-transcriptional gene silencing signals moved freely throughout the symbiont and slightly into adjacent stem tissue. Under tested field and greenhouse conditions in potato and tomato, respectively, gall or symbiont formation had no negative impacts on plant growth or tuber and fruit yield. In vitro, symbiont cultures abundantly secreted recombinant protein into surrounding media. Together, these results establish symbionts as a modular, plant bioengineering platform capable of producing and potentially delivering biomolecules without modifying the host plant genome, providing a foundation for vascular-targeted therapeutics and phenotype modulation in crops.

This article investigates the intellectual, literary, and historical contributions of Sindhi poets and writers to the development and enrichment of Urdu language and literature. It situates the emergence of Urdu in Sindh within a broader historical continuum, beginning with the Arab conquest of Sindh and extending through subsequent Iranian cultural influences. These encounters facilitated a dynamic linguistic and cultural exchange, laying the groundwork for the evolution of Urdu as a literary medium in the region. The study critically examines the role of early Sindhi Sufi poets, particularly Shah Karim, whose Persian poetry reflects a deep metaphysical tradition that later informed both Sindhi and Urdu literary expressions. It further explores the translational and interpretive endeavors of modern Sindhi intellectuals, such as Shaikh Ayaz, whose rendering of Shah Abdul Latif Bhittai’s poetry into Urdu represents a significant act of cultural mediation, making Sindhi mystical thought accessible to a wider readership. A key focus of the paper is the impact of indigenous Sindhi poetic forms, especially the Waaee and Kaafi, on the thematic and stylistic dimensions of Urdu poetry. These forms, rooted in Sufi philosophy and oral traditions, have contributed to the evolution of Urdu poetic sensibilities, particularly in terms of simplicity, musicality, and spiritual symbolism. In this context, the Urdu poetry of Sachal Sarmast is analyzed as a vital bridge between Sindhi and Urdu literary traditions, exemplifying linguistic plurality and mystical universality. The research also engages with the scholarly contributions of Dr. Nabi Bux Baloch, whose seminal work on Urdu poetry in Sindh provides a foundational framework for understanding the historical trajectory of Urdu in the region. Additionally, the article highlights the translation of Sindhi short stories into Urdu, which has played a crucial role in cross-cultural literary exchange and the dissemination of Sindhi narratives within the broader Urdu literary canon. Finally, the study evaluates the contributions of contemporary Sindhi writers and poets who have actively participated in Urdu literature, thereby reinforcing the interconnectivity between the two languages. Through a comprehensive and interdisciplinary approach, this article underscores Sindh’s pivotal role as a cultural and linguistic bridge, demonstrating how Sindhi literary heritage has significantly enriched Urdu language and literature across historical and modern contexts.

Introducción: La escoliosis es una deformidad frecuente en niños con parálisis cerebral (PC), es progresiva y afecta negativamente la capacidad funcional. A nivel internacional, curvas escolióticas sobre 45° grados reciben cirugía correctiva como tratamiento para mantener la curva escoliótica y evitar su progresión; a nivel local se utiliza un tratamiento conservador con ortesis toraco-lumbo sacra (TLSO). Al respecto, el presente estudio buscó comparar la evolución de la escoliosis en pacientes con tratamiento de TLSO durante 12 meses con la historia natural de la escoliosis. Materiales (o pacientes) y Métodos: Estudio de dos cohortes dinámicas de pacientes con diagnóstico de PC, escoliosis mayor a 20° e indicación de TLSO entre enero de 2017 y diciem­bre de 2019 en el Instituto Teletón (IT) Santiago. Durante un año se evaluó la curva escoliótica mediante método Cobb y tiempo mediano transcurrido por presencia de evento adverso (cambio de TLSO). Resultados: Se identificaron diferencias, estadísticamente significativas, en la curva escoliótica entre la medición basal y el primer control, y entre el primer control y el último. No hubo variables demográficas o clínicas asociadas, significativamente a las variaciones de la curva escoliótica. Se identificó una diferencia importante entre las curvas de sobrevivencia de quienes presentaron un evento adverso y aquellos que no. Conclusiones: El TLSO detuvo la progresión de la curva escoliótica tras 12 meses de uso. La falta de casos comparables impidió con­trastar los resultados obtenidos con la historia natural de la escoliosis descrita en el antecedente teórico disponible.

The interaction between fasting plasma glucose (FPG) and fasting insulin (FI) concentrations and diets with different carbohydrate content were studied as prognostic markers of weight loss as recent studies up to 6 months of duration have suggested the importance of these biomarkers. This was a retrospective analysis of a clinical trial where participants with obesity were randomized to an ad libitum low-carbohydrate diet or a low-fat diet with low energy content (1200-1800 kcal/day [≈ 5.0-7.5 MJ/d]; ≤ 30% calories from fat) for 24 months. Participants were categorized (pretreatment) as normoglycemic (FPG < 5.6 mmol/L) or prediabetic (FPG ≥ 5.6-6.9 mmol/L) and further stratified by median FI. Linear mixed models were used to examine outcomes by FPG and FI values. After 2 years, participants with prediabetes and high FI lost 7.2 kg (95% CI 2.1;12.2, P = 0.005) more with the low-fat than low-carbohydrate diet, whereas those with prediabetes and low FI tended to lose 6.2 kg (95% CI -0.9;13.3, P = 0.088) more on the low-carbohydrate diet than low-fat diet [mean difference: 13.3 kg (95% CI 4.6;22.0, P = 0.003)]. No differences between diets were found among participants with normoglycemia and either high or low FI (both P ≥ 0.16). Fasting plasma glucose and insulin are strong predictors of the weight loss response to diets with different macronutrient composition and might be a useful approach for personalized weight management.

To examine the association of program delivery factors with weight loss (WL) in enrollees in iDiet® , a commercial WL program. Data were from 644 adults enrolling in an 11-week group WL program and 461 who reported weight to 11 weeks (complete reporters). Predictors of %WL were analyzed using ANCOVA, including meeting type (in-person vs. videoconference), participant type (worksite employees vs. community members), age, gender, BMI, and payment structure. Mean starting BMI was 32.4 ± 7.1 (mean ± SD); WL was 6.1 ± 3.9% in all enrollees in an intention-to-treat analysis and 7.4 ± 3.4% in complete reporters. Videoconference participants, older adults, and enrollees in incentivized programs were more likely to be complete reporters (P < 0.004). %WL at 11 weeks was not associated with gender, starting BMI, or videoconference versus in-person groups. Worksite participants had greater %WL than community participants (+1.2%, P < 0.001), and there was no significant difference in %WL between programs paid by the employee or employer. Greater %WL was achieved by individuals ≥30 versus < 30 years (+2.2%, P < 0.001) and by those enrolling in January-March versus April-June (+1.4%, P = 0.02). iDiet participants had clinically impactful mean WL. The observed high mean WL in worksites and videoconference-delivered programs broadens options for scalable WL program implementation.

WINDGRABBER™ (WG) is a novel new roof or pole top mounted wind turbine system proposed for product commercialization up to 50 kWe. WG is projected to be cost-attractive in an environmentally friendly manner. The WG system uses the available energy in the wind via (1) a passively yawed air impact inlet air scoop, (2) a flow tube, (3) an air turbine and (4) a multiphased air inlet with an air impact - drag type outlet section. The WG wind turbine system preferentially utilizes a combination enclosed - radial out-flow, cross-flow, reaction-impulse air turbine of squirrel cage configuration, using either a single or double axial flow inlet of centrifugal fan design with backwardly curved, air foil air blades mounted on the discharge end of a flow tube. The WG wind turbine design is based on the air power instead of the wind power equations, combined with duct resistance, wind impact and wind drag calculations, to determine system wind energy conversion effectiveness with respect to the Betz limit, as well as overall system efficiency. Low pressure drop screens may be provided at the various inlets and outlets to protect birds and small mammals from being drawn into rotating components. A currently proposed Phase I – CAE modeling for conceptual design verification, system optimization and 300 watt pilot plant test program, followed by a Phase II – design improvement and scale up to 3,000 watt prototype demonstration program and followed by a Phase III cost reduction, product improvement and scale up to a 5,000 watt commercial demonstration program is currently being considered by BCK Consulting, L.L.C. (BCK) and West Texas A &amp; M University (WTAMU). The history of WG, up to and including its latest developmental status, will be discussed in this paper with projections offered regarding its future product commercialization prospects. Please see ASME paper ES2010-90062 presented previously [Ref.1].

Abstract Electronic packaging is a multidisciplinary process consisting of the physical design, product development, and manufacture required to transform an electronic circuit schematic diagram into functional electronic equipment. There are four basic categories of concern to the electronic packaging engineer, working from the external environment inward: exterior conditions, internal conditions, component environments and component requirements. Several computer‐aided design programs address electronic packaging design and development tasks. Most computer algorithms are adaptations of codes generated for other, related purposes (e.g., finite‐element techniques for structures, stresses, thermal analysis, fluid flow analysis, and flow visualization; solid‐modeling and drafting programs; printed circuit board design programs). Electronic packaging applications often fall into one or more of the following categories. They are equipment attachment, equipment racks, frames, and mounting structures, equipment and module enclosures, temperature control, mechanical joints, finishes, position‐sensitive assemblies, electrical contacts, and harsh‐environment endurance.

In the era of information society, IT industry has been developed very much. New technology has made appearance in citizens' lives. IOT (Internet of Things) has grown up the most rapidly in IT industry. Kevin Ashiton, MIT specialist, said, "Loading of FRIS and other sensors shall build Internet of things." Internet of things is said to let things have sensor and communication module and to exchange information and communicate each other. In this study, Internet of things has been applied to flowerpot to build automatic flowerpot control system that turns fan ON and supplies water depending upon temperature and moisture. Users are difficult to cognize temperature and humidity of flower pot correctly. In this study, an experiment obtained correct value of temperature and humidity to build control system. At the performance test of flower pot, commands turned ON depending upon temperature and humidity. Control system should be added to control water supply quantity and time objectively according to servo motor control. Purpose of further study was to control flower pot by remote system in connection with smart phone application. An application control can make not only temperature and humidity statistics but also server depending upon users' needs to turn fan ON and take actions and to control flower pot.