Theodore Roosevelt High School

Exploring genetic tools in environmental microbes: Applications in extracellular electron transfer Arpita Bose and Zhecheng Zhang explore genetic tools in environmental microbes, citing applications in extracellular electron transfer. This article reviews three main genetic approaches that have expanded our ability to study electroactive bacteria, particularly those that are not traditional model organisms but can perform extracellular electron transfer (EET). The first approach is CRISPR genome editing, a method that allows for targeted gene knockouts for specific modifications in redox-related pathways.

Chest X-Ray Interpretation in the EPICO Pediatric COVID-19 Study: Addressing MisconceptionsLuis Miguel Navarro‐Ramirez¹˒² | Pablo Vásquez‐Hoyos³˒⁴ | María Lucía Mesa‐Rubio⁵ | Melisa Naranjo Vanegas¹˒⁶ | Daniela Duarte-Montero¹˒⁷ | Claudia Burgos⁵ | Laura Melissa Mendez⁵ | María Margarita Rodriguez⁵ | Arianna Martinez⁵ | Paola Sanchez⁵ | Carolina Tovar⁵ | Gabriela Friedrich¹ | Gustavo Adolfo Triana‐Rodriguez⁸ | Mónica Royero‐Arias⁹ | Jessica Echeverry¹⁰ | Tamara Gamo¹⁰ | Luz Ángela Moreno⁴˒¹⁰ | Olga Lucía Baquero¹¹ | Luz Marina Mejía¹² | Sonia Restrepo‐Gualteros⁵ | Sergio Moreno‐Lopez¹ | Juan Gabriel Piñeros⁵ | Carlos Álvarez‐Moreno⁴˒¹³ | Alejandro Díaz‐Díaz¹⁴˒¹⁵ | Iván Felipe Gutierrez¹⁶ | Clara Galvis‐Diaz¹⁷ | José Manuel Nieto¹⁸ | Irati Gastesi¹⁹ | Cinta Moraleda¹⁹ | Alfredo Tagarro García¹⁹ | Andrea Ramirez Varela²⁰˒²¹˒²² | EPICO‐Colombia and EPICO‐AEP working groups¹ School of Medicine, Universidad de los Andes, Bogotá D.C., Colombia ² Craig Dalsimer Division of Adolescent Medicine, Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania, USA ³ Red Colaborativa Pediátrica de Latinoamérica (LARed Network), Montevideo, Uruguay ⁴ School of Medicine, Universidad Nacional de Colombia, Bogotá D.C., Colombia ⁵ Department of Pediatrics, Fundación Santa Fe de Bogotá, Universidad de los Andes, Bogotá D.C., Colombia ⁶ Medical Imaging & AI Group - Bioscience Center, Ayudas Diagnósticas Sura, Medellín, Antioquia, Colombia ⁷ PediAFe Research Group, Fundación Santa Fe de Bogotá, Universidad de los Andes, Bogotá D.C., Colombia ⁸ Department of Diagnostic Imaging, Fundación Santa Fe de Bogotá, Bogotá D.C., Colombia ⁹ Pediatric Radiology, Servicios de Salud San Vicente Fundación, Medellín, Antioquia, Colombia ¹⁰ HOMI, Fundación Hospital Infantil de la Misericordia, Bogotá D.C., Colombia ¹¹ Clínica Infantil Colsubsidio, Bogotá D.C., Colombia ¹² Instituto de Ortopedia Infantil Roosevelt, Bogotá D.C., Colombia ¹³ Clínica Universitaria Colombia, Clínica Colsanitas, Bogotá D.C., Colombia ¹⁴ Pediatric Infectious Diseases, Hospital Pablo Tobón Uribe, Medellín, Antioquia, Colombia ¹⁵ Pediatric Infectious Diseases, Hospital General de Medellín, Medellín, Antioquia, Colombia ¹⁶ Pediatric Infectious Diseases, Clínica Infantil Santa Maria del Lago, Clínica Colsanitas, Bogotá D.C., Colombia ¹⁷ Hospital Militar Central, Bogotá D.C., Colombia ¹⁸ Hospital Regional de la Orinoquía, Yopal, Casanare, Colombia ¹⁹ Fundación Investigación Biomédica Hospital Universitario 12 de Octubre (FIBH12O), Instituto de Investigación Hospital 12 de Octubre (imas12), Madrid, Spain ²⁰ Department of Epidemiology, School of Public Health, The University of Texas Health Science Center at Houston, Houston, Texas, USA ²¹ Department of Pediatrics, McGovern Medical School, University of Texas Health Sciences Center at Houston (UTHealth), Houston, Texas, USA ²² School of Public Health, Center for Health Equity, University of Texas Health Science Center at Houston, Houston, Texas, USACorresponding author: Luis Miguel Navarro-Ramirez | Lm.navarror@uniandes.edu.coAddress for reprint: Universidad de los Andes, School of Medicine, Carrera 7 # 116-05, Bogotá DC, Colombia.To the editor,We appreciate the opportunity to respond to the commentary by Daungsupawong and Wiwanitkit regarding our manuscript, “What to Look for in Chest X-Rays of Pediatric Patients With COVID-19: Insights From a Colombian Cohort” [1,2]. We thank the authors for their interest in our work and their thoughtful engagement. Several of the concerns raised in the commentary were, in fact, addressed in the original manuscript; nonetheless, we welcome the opportunity to clarify these points and provide additional context where needed.

This study endeavors to determine the connection between emotional well-being and academic performance among college students at FEU Roosevelt. Using a correlational quantitative research design, the study aims to determine the strength and direction of the correlation between the students’ emotional well-being measured by the Warwick-Edinburgh Mental Well-being Scale (WEMWBS), and their academic achievement measured via self-reported Grade Point Average (GPA). The respondents were randomly selected by stratified random sampling from different academic programs, including very competitive ones such as Business Administration and Information Technology. An internet-based self-report survey was used for data collection, and descriptive statistics and the Pearson product-moment correlation coefficient were used for data analysis using SPSS. An inverse correlation exhibiting high significance was found between emotional well-being and academic performance; students who study in more demanding programs show lower emotional well-being and poorer academic performance. These results are in line with current literature which identifies stress and emotional tension as contributory factors to academic failure, especially in high-stress academic environments. The research highlights the importance of universities adopting proactive mental health care and providing individualized interventions for high-stress courses, particularly for students enrolled in such courses. This study contributes to the body of knowledge about emotional well-being and academic performance in Philippine tertiary institutions.

ABSTRACTHeadwater streams are abundant in mountain landscapes and where municipal watersheds occur on public lands; they co‐occur with road networks open to off‐highway vehicle (OHV) use. Despite well‐known, deleterious interactions of roads and streams, few studies have empirically examined the biophysical impacts of OHV use where backcountry roads enter streams at open‐water crossings. We present a case study using a controlled design where we quantified OHV road impacts in a Rocky Mountain stream and evaluated the restoration efficacy by examining aquatic biota and habitat before and after restoration treatments. Prior to restoration, stream habitat structure was simplified and the abundance, biomass, and diversity of aquatic biota were severely reduced in OHV‐affected compared to reference reaches. Restoration included the removal of riparian OHV roads, rebuilding streambanks and bedforms, and additions of large wood. Following restoration and 2 years of spring snow‐melt runoff, some, but not all, aquatic habitat had recovered, and aquatic biota were more similar to conditions in reference reaches. We included a vulnerability assessment for streams draining ~5 M Ha of public lands in the Rocky Mountains, indicating the greatest threat of similar OHV impacts to 2nd‐ and 3rd‐order streams in watersheds deemed important for municipal supplies. Our study provides insights that may aid in effective prevention approaches and restoration of similar sites.

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Introducción: Existe un vacío conceptual sobre la realización temprana de la confirmación genética para la Atrofia Muscular Espinal (AME). Se propone, por medio de un panel Delphi modificado, un algoritmo que oriente pragmáticamente al personal de salud, en la identificación temprana de los signos y síntomas de AME 5q tipos 1 a 3, con el fin de incrementar el rendimiento diagnóstico, clínico y molecular. Materiales y Métodos: Utilizando Delphi modificado, tres expertos con amplia experiencia clínica, investigativa y docente desarrollaron 13 casos, teniendo como insumo una revisión descriptiva de la literatura y su experiencia asistencial. Posteriormente, un grupo extendido de nueve expertos participó en dos rondas calificativas para cada caso utilizando la escala Likert. Resultados: Ocho expertos completaron la primera ronda, reportaron una mediana de ejercicio asistencial como especialista de 22.5 años (rango 5 a 35) y una mediana de pacientes con AME tratados de 50 (rango 15 a 150). En la primera ronda, se logró consenso en 19 afirmaciones para AME-1, 19 afirmaciones para AME-2 y 15 afirmaciones para AME-3. En la segunda ronda se logró consenso para todas las afirmaciones de examen físico y paraclínicos, mientras que las afirmaciones sin consenso se relacionaron a diagnósticos diferenciales. Hubo consenso en 72.7% de 11 afirmaciones para AME-1, en 85.7% de ocho afirmaciones para AME-2 y en 100% de ocho afirmaciones para AME-3. Conclusiones: La metodología Delphi permitió configurar una aproximación algorítmica en forma de flujograma para la identificación temprana de los signos y síntomas y el diagnóstico de cada subtipo de AME 5q. Se propone un sustento integral y pragmático que sirva de orientación para el médico general y especialista, enfatizando en la importancia de su capacitación en la evaluación de signos y síntomas para considerar esta enfermedad tempranamente en el diagnostico diferencial.

Intermittent rivers are dynamic ecosystems increasingly affected by climate change which extends their dry phase and alters connectivity. Drying events create temporal dispersal corridors for terrestrial animals including pest taxa with potential agricultural and forestry impacts. We aimed to investigate dispersal of thrips (Thysanoptera) and bark (and ambrosia) beetles (Scolytinae) in the Mediterranean karst intermittent river Krčić, Croatia, during its dry phase by assessing spatiotemporal distribution across habitats, reaches and times of day as well as the wind effect on their activity density. Insects were sampled using cross-vane window traps in riparian and dry riverbed habitats at upper and lower reaches. Traps were emptied every 12 h over seven days in July 2021, with wind continuously monitored. Thysanoptera activity density was significantly higher in the upper reaches and during the day; light wind enhancing their dispersal, but they showed no clear habitat preference. Scolytinae preferred riparian habitat relative to the dry riverbed, likely due to differences in environmental conditions, such as temperature, light intensity and humidity. While these taxa are commonly studied in relation to their host plants, this study highlights their ecology, contributing to a better understanding of how prolonged dry periods in intermittent rivers affect biological communities.

ABSTRACT The central star of the Helix Nebula, WD 2226$-$210 presents enigmatic hard X-ray emission and mid-IR excess. The latter has been attributed to a dusty disc or a cloud-like structure around WD 2226$-$210 formed from material of Kuiper Belt-like or comet-like objects in highly eccentric orbits. We present here a detailed analysis of multi-epoch Chandra and XMM–Newton X-ray observations of WD 2226$-$210, comparing these to previous Einstein and ROSAT data. The luminosity of the hard X-ray component of WD 2226$-$210 has remained basically constant in the decade from 1992 to 2002, with very subtle evidence for variability in time-scales of hours. Under the assumption that the X-ray emission from WD 2226$-$210 is due to accretion of material, an accretion rate of $\dot{M}\approx 10^{-10}$ M$_\odot$ yr$^{-1}$ is estimated. The origin of the material accreted by WD 2226$-$210 is uncertain, and can be attributed to the disc-like structure around it or to a substellar donor companion. The accretion rate proposed for the continuous replenishment by bombardment of the mid-IR-emitting structure around WD 2226$-$210 cannot match that required by the X-ray emission.

Abstract. Air quality is crucial for health, yet monitoring airborne particulate matter (PM), particularly PM2.5, remains challenging due to pollution and widespread distribution. PM2.5, with diameters less than 2.5 micrometers, are known to have significant health implications. Traditional methods of PMs detection and analysis are often time-consuming or require complex optical systems. This study explores the use of lensless microscopy as an innovative and cost-effective alternative for the visualization and measurement of PMs. Utilizing computational imaging techniques, lensless microscopy captures high-resolution images without the need for traditional lenses, facilitating rapid and accurate analysis of air quality. We describe the implementation of lensless imaging to detect and quantify PM2.5 particles in the air, highlighting the use of the single light wavelength and the computational reconstruction method. Our experiments demonstrated that this approach could effectively capture fine details of PMs, achieving resolutions comparable to traditional microscopy. This validates the potential of lensless microscopy as a practical tool for real-time air quality monitoring. By adopting lensless microscopy, we offer a more efficient and economical method for the continuous monitoring of PMs, which can significantly contribute to environmental health studies and pollution control efforts. According to specific needs, simply replacing the flow rate of the air pump can adapt to the PM measurement speed requirements in different environments. This research introduces a novel approach to air quality assessment, providing a promising solution for the rapid and detailed analysis of airborne particulate matter while at an affordable cost.