Universidad de Occidente

The reconstruction of extensive skin defects remains a major challenge in plastic and reconstructive surgery, largely due to the limited vascularization and integration capacity of conventional skin grafts and substitutes. In recent years, three-dimensional bioprinting has emerged as a promising biofabrication strategy capable of addressing these limitations through precise spatial organization of cells, biomaterials, and vascular architectures. This review analyzes current advances in the bioprinting of vascularized skin grafts, with particular emphasis on vascularization strategies, cellular composition, bioink selection, and features relevant to translational application. The findings indicate a clear trend toward hybrid vascularization approaches that combine predefined architectural guidance with endothelial cell–driven self-organization, enabling improved perfusion and viability in thicker constructs. Tri-cellular configurations incorporating keratinocytes, fibroblasts, and endothelial cells are most frequently reported, reflecting efforts to recreate essential skin–vascular interactions. Material strategies increasingly favor decellularized extracellular matrix–based and hybrid bioinks to balance biological fidelity with mechanical stability. Although significant progress has been made toward functional and perfusable skin constructs, challenges remain regarding surgical handling, long-term vascular stability, and standardized personalization workflows. Overall, vascularized bioprinted skin grafts represent a rapidly evolving platform with strong potential to support personalized reconstruction, bridging experimental biofabrication and future reconstructive practice.

Flow cytometric immunophenotyping is essential for the diagnosis and immunologic classification of B-cell acute lymphoblastic leukemia in children and is traditionally performed using bone marrow aspirates, an invasive procedure that may be delayed or unavailable in certain clinical contexts. However, many patients present with circulating blasts at diagnosis, raising the possibility of using peripheral blood as an alternative source for initial immunophenotypic classification. Although previous studies have shown that peripheral blood can support initial diagnostic classification, they have not determined the degree of marker-level concordance between peripheral blood and bone marrow nor the clinical conditions under which this concordance is strongest. In this study, we evaluated the immunophenotypic concordance between peripheral blood and bone marrow in 32 pediatric patients with B-cell acute lymphoblastic leukemia, using paired samples obtained at diagnosis and analyzed with a standardized panel of B-lineage and maturation markers. Accordingly, the objective of this study was to quantify marker-specific agreement between compartments and identify clinical factors associated with higher concordance. Overall concordance was moderate across all subpopulations (mean CCC ~0.63) but higher for the markers most relevant to routine diagnostic classification (e.g., CD19+, CD10+, CD34+, and HLA-DR+), several of which exceeded 95% concordance and showed minimal bias between specimens. These findings apply only to newly diagnosed B-ALL with sufficient circulating blasts, as cases with minimal residual disease, low-blast presentations, atypical immunophenotypes, or mixed-lineage leukemias were not included in this study. The greatest concordance was seen in patients with hemoglobin < 8 g/dL, in whom extensive marrow infiltration promotes blast spillover into circulation. Likewise, patients older than 10 years showed high concordance, consistent with greater leukemic burden at presentation. Even in subgroups with lower circulating blast levels, such as those with hemoglobin ≥ 8 g/dL, peripheral blood adequately reproduced most leukemic cell populations present in bone marrow. Overall, these findings indicate that the markers and subpopulations most relevant for diagnostic immunophenotyping can be reliably assessed using peripheral blood in high-burden disease settings, reducing the immediate need for invasive procedures and facilitating timely immunophenotypic classification, particularly in resource-limited environments or situations where rapid initiation of treatment is critical.

La presente investigación surge a partir de una preocupación por el aumento significativo de personas que experimentan altos niveles de estrés debido a su trabajo, especialmente en el ámbito educativo, siendo evidente que dichos niveles de estrés pueden llevar a los profesores a presentar el síndrome de Burnout. Se optó por utilizar el enfoque cualitativo, que permite abordar la realidad de las poblaciones de estudio de diversas formas para comprender mejor el fenómeno estudiado. Así mismo, se aplicó el método etnográfico para describir y explicar las circunstancias y características del grupo de maestros con Burnout, debido a que este permite interactuar con la comunidad para obtener datos relacionados con su organización, cultura, costumbres, creencias y otros aspectos relevantes. Los datos se recolectaron a través de entrevistas semiestructuradas, encuestas y observaciones no participantes. El escenario donde se llevó a cabo la investigación fue el ITLM Campus Villa de Ahome, con 20 maestros y la población estudiantil con la que cuenta en la institución que proviene de pueblos aledaños a la Villa de Ahome. Esta investigación se centró en indagar los síntomas del Burnout en los profesores, la interacción de estos con los alumnos, los factores desencadenantes, así como los efectos del Burnout en el aprendizaje, demostrando la necesidad de abordar este problema en el contexto educativo, ya que conocer el Burnout en las escuelas permite identificar factores de riesgo y estrategias de prevención, mejorando la calidad de vida de los docentes y el proceso de aprendizaje de los estudiantes. Por lo tanto, la gestión escolar puede implementar programas de apoyo y estrategias para reducir el estrés y promover el bienestar emocional de los docentes, creando ambientes saludables que favorezcan el aprendizaje de los alumnos.

The genus Microbacterium contains bacteria ubiquitously distributed in various environments and includes plant-associated bacteria able to colonize the tissue of agricultural crop plants. The objective of this work was to complete the genome sequence of Microbacterium enclense GM-13 isolated from a field-grown bean in Sinaloa. Here, we report the complete genome sequence of Microbacterium enclense GM-13, isolated from a conventionally grown bean (Phaseolus vulgaris) from a field in Culiacán, Sinaloa, Mexico. The nucleotide sequence of this genome was deposited into NCBI GenBank under the accession PRJNA1168145. In this report, we introduce the entire genomic sequence of Microbacterium enclense GM-13, which is 3,172,320 bp long, with 71.74% G + C content and 3,076 CDSs. The Microbacterium enclense GM-13 genome is essential for understanding the genetics of this pathogen, which will aid in analyzing possible virulence genes and contribute to the development of control strategies.

This paper presents the design of a posture-based reference signal for assistance control in electric bicycles. Unlike traditional approaches that rely on pedaling or the use of a throttle, this proposal is based on the use of load cells located on the seat of the electric bicycle to estimate the rider’s posture. The information from the load cells is processed, and using the k-means clustering algorithm, a centroid representing the user’s inclination is determined. Depending on the location of this centroid within a previously calibrated assistance zone, a voltage signal proportional to the required assistance intensity could be generated. The results show that this system allows differentiating between postures that indicate intention to assist and those that do not, offering a personalized, ergonomic and safe alternative for assistance control. Future work contemplates the implementation of the system on a functional electric bicycle to evaluate the experience of real users and adjust the assistance zone. This approach can contribute to the development of solutions for sustainable urban mobility.

We present a case of a woman in her mid-30s at 21 weeks of gestation referred to our tertiary medical center by an outside clinic with an ultrasound finding of a cystic septated lesion on the left side of the fetal chest.The differential diagnosis included, among others, foetal nuchal oedema, cystic hygroma, meningocele, encephalocele, cervical teratoma, haemangioma and subchorionic placental cyst. The sonographic finding of lack of intralesional vascularity on Doppler, seemingly without communication with the spinal canal, pointed us towards a diagnosis of foetal cystic lymphangioma. Our diagnosis was confirmed postnatally.Foetal cystic lymphangiomas are rare benign congenital tumours of the vascular and lymphatic systems. The differential diagnosis is broad, and a timely diagnosis positively affects the prognosis. If a lymphangioma is suspected, we suggest referral to specialists in maternal–foetal medicine with expertise in the field.

The objective of the invitation is to detail the experience of spiritual practices and their impact on the well-being of the followers of evangelical Christianity belonging to the community of the Church in Los Mochis, Sinaloa. The approach used for this research was of a mixed nature with a predominance of the qualitative approach, implementing the phenomenological method, with a sequential design, in the first phase the quantitative sphere of the research is attended to by determining the level of well-being of the participants and the second phase, the qualitative sphere is attended to that has the purpose of studying and describing the experience in spiritual practices. To collect information, the descriptive questionnaire for spiritual experience was created to allow participants to describe their experience and thus inquire about their experience of spirituality, to know the practices and activities that the believer performs, it is a questionnaire of 21 open questions and is oriented to the description of these experiences, for this, the components for each variable found in the literature were taken into account. The MHC mental health continuum questionnaire was implemented to measure the well-being of believers, this instrument was developed by Dr. Keyes (2002). The results of the MHC (Measurement of Well-Being) mental health continuum questionnaire show average general well-being for the practitioners of evangelical Christianity in the JN church of Los Mochis, however, they score high well-being in psychological well-being (eudemonia within the Keyes model) and social well-being, maintaining average well-being in hedonic (experiences of effects, positive emotions); the code with the most citations was the one referring to affections with 31 (citations), followed by Interpersonal Relationships with 25, concept with 22, personal growth with 17, connection and meaning with 15, as well as certainty/hope with 14 and self-concept with 13. As for the relationship between categories, a wide relationship can be observed between practices and activities, personal relationships, and the experience of positive emotions, another point to highlight is that the search for transcendence and knowledge about a supreme being gives them meaning and purpose in life, which in turn is related to personal growth and therefore to self-concept.