Universidad del Golfo de México

Background: Knee pathology is common and affects quality of life. The most frequent lesions are synovitis, bursitis, trochlear cartilage damage, and tendinopathies, with cartilage wear being relevant due to its relationship with mechanical overload. Early detection is key, and ultrasound, due to its accessibility, allows identification of the etiological cause of pain. Objective: To determine knee ultrasound findings associated with pain in patients aged 30–60 years at HGR 1 Orizaba, Veracruz. Material and Methods: Descriptive, cross-sectional study in beneficiaries aged 30–60 years, with and without knee pain. Philips ultrasound was applied with a high-frequency linear transducer (7.5–12 MHz), evaluating synovitis, bursitis, tendinitis, meniscal lesions, soft tissue inflammation, and cartilage changes. Pain intensity was measured with VAS (0–10). Sociodemographic and clinical data were collected (age, sex, BMI, diabetes, physical activity). Descriptive and inferential statistics were used (Chi-square). Period: August–December 2025. Results: The most frequent findings were cartilage changes (49%) and bursitis (44%), followed by tendinitis, meniscal lesions, and synovitis. In patients with pain, prevalence was higher: bursitis (84.4%), cartilage (80%), tendinitis (39%), meniscal lesions (37.9%), and synovitis (12.3%). Bilateral pain predominated (78.3%). Intensity showed a dose–response relationship: higher VAS was associated with more findings. Older age and obesity increased risk, while sex and diabetes were not significant. Mild physical activity predominated (62%) and was associated with more pain; vigorous activity showed a protective effect. Sports practice and active work were associated with lower prevalence of findings. Conclusions: Knee pain in adults is associated with ultrasound findings, mainly cartilage changes and bursitis. A dose–response relationship between VAS and structural damage was confirmed, with elevated risks for tendinitis and meniscal lesions. Age and obesity were determining factors, while vigorous and moderate physical activity showed a protective effect. Ultrasound is reaffirmed as a useful diagnostic tool and exercise as a preventive strategy to reduce musculoskeletal load.

The sponge is one of the potential sources of bioactive compounds. Among them, the sponge Aaptos has been a promising discovery of leading drugs including aaptamine (1). This study investigated the aaptamine (1), spectroscopically determined, from Aaptos sp. collected from Bunaken National Park, Indonesia, as an anticancer agent, specifically targeting colorectal cancers (CRCs). Compound 1 showed potent cytotoxicity against DLD-1 and Caco-2 with IC50values of 30.3 and 236.8µg/mL, respectively. In addition, the exposure of compound 1 on those colorectal cancer cells could promote cell cycle arrest and relatively induce necrotic cell death.

This research examines changes in Prabowo Subianto's political image and a campaign approach that is more attractive to the younger generation, especially millennials and Gen Z, in the context of the 2024 election in Indonesia. With a focus on the use of social media and innovative communication strategies, this research explores how candidate pair Prabowo and Gibran Rakabuming Raka use 'gemoy' imagery and creative gimmicks to create a unique impression and increase their popularity among young voters. Prabowo and Gibran's communication strategies are explained using PESTEL analysis, AIDA, communication strategy decisions, and the 6C Model. Research findings show that this approach is effective in attracting the attention, increasing popularity, and participation of young voters, who are an important segment in elections. Keyword: marketing communication strategies, campaign advertising, social media

Community acquired pneumonia (CAP) merupakan peneumonia yang berasal dari komunitas yang merupakan infeksi akut parenkim paru meliputi aveolus dan jaringan intestinal yang disebabkan oleh mikroorganisme. Penggunaan antibiotik empirik di setiap negara dapat berbeda-beda sesuai dengan sensitifitas bakteri maupun pola kuman di negara tersebut. Penelitian ini bertujuan untuk mengevaluasi penggunaan terapi antibiotik empirik serta hubungannya terhadap luaran klinik pada pasien anak dengan CAP dirawat inap RSA UGM Yogyakarta. Penelitian ini merupakan penelitian observasional dengan rancangan cohort retrospektif. Subjek dalam penelitian ini adalah pasien anak dengan CAP dirawat inap RSA UGM Yogyakarta pada periode Januari 2021 – Januari 2022. Data pada penelitian ini bersumber dari catatan medik RSA UGM, selanjutnya dilakukan analisis hubungan antara ketepatan penggunaan antibiotik empirik dengan luaran klinik menggunakan analisis chi-square. Jumlah pasien pada 1 Januari 2021 – 1 Januari 2022 didapatkan total 90 pasien kemudian yang memenuhi kriteria inklusi sebanyak 69 pasien dengan 80 regimen terapi antibiotik empirik. Sebanyak 55 pasien dengan umur < 5 tahun, pasien pada penelitian ini lebih banyak ditemukan pada jenis kelamin perempuan sebanyak 39 pasien. Pasien pada penelitian ini paling banyak menggunakan terapi tunggal dengan antibiotik sefalosporin generasi 3 yakni seftriakson sebanyak 38 pasien, terapi kombinasi yang paling banyak adalah ampisilin dan gentamicin sebanyak 8 pasien. Berdasarkan penilaian Gyssens sebanyak 30 pasien menerima terapi antibiotik empirik dengan tepat. Berdasarkan uji chi-square terdapat hubungan antara ketepatan antibiotik empirik dengan luaran klinik pasien dengan nilai p= 0,039 (p= <0,05) sehingga dapat disimpulkan terdapat pengaruh ketepatan penggunaan antibiotik empirik terhadap luaran klinik pasien

Strawberries contain antioxidants, including quercetin-3-β-D-glucoside, coenzyme Q10, anthocyanins pelargonidin-3-O-glucoside, and cyanidin-3-glucoside. The amount of anthocyanins and flavonoids in fruit tends to decrease by 40-50% after the formulation process, so it is necessary to develop a formula to be applied as a topical preparation. The combination of emulsifiers can stabilize the active substance and increase the permeation of the preparation. This study aims to determine the total flavonoid permeation profile of strawberry fruit emulgel preparations with a combination of emulsifiers. This study used a total flavonoid model from strawberry emulgel. Emulgel was prepared with a combination of emulsifier span 80: croduret 50: propylene glycol at a ratio of 2: 4: 2. The permeation test was carried out using a modified vertical Franz diffusion cell equipped with Shed Snakeskin membrane. The permeation test results showed that the cumulative amount of total flavonoids transported across the shed snakeskin membrane was 117.14 g/cm2 out of a total of 2.24 mg of strawberries in the emulgel formula for 5 hours. The permeability of the snakeskin shed membrane was 2.84x10-5 g/cm2, and the flux value was 6.6x10-5 g/sec. The release kinetics follow the Higuchi kinetic model with the diffusion mechanism.

Abstract Many challenges of today's science are parametric optimization problems that are extremely complex and computationally intensive to calculate. At the same time, the hardware for high-performance computing is becoming increasingly powerful. Geneva is a framework for parallel optimization of large-scale problems with highly nonlinear quality surfaces in grid and cloud environments. To harness the immense computing power of high-performance computing clusters, we have developed a new networking component for Geneva, the so-called MPI Consumer, which makes Geneva suitable for HPC. Geneva is most prominent for its evolutionary algorithm, which requires repeatedly evaluating a user-defined cost function. The MPI Consumer parallelizes the computation of the candidate solutions' cost functions by sending them to remote cluster nodes. By using an advanced multithreading mechanism on the master node and by using asynchronous requests on the worker nodes, the MPI Consumer is highly scalable. Additionally, it provides fault tolerance, which is usually not the case for MPI programs but becomes increasingly important for HPC. Moreover, the MPI Consumer provides a framework for the intuitive implementation of fine-grained parallelization of the cost function. Since the MPI Consumer conforms to the standard paradigm of HPC programs, it vastly improves Geneva's user-friendliness on HPC clusters. This article gives insight into Geneva's general system architecture and the system design of the MPI Consumer as well as the underlying concepts. Geneva, including the novel MPI Consumer, is publicly available as an open source project on GitHub and is currently used for fundamental physics research at GSI in Darmstadt, Germany.

As chemotherapeutic backbone for breast cancer therapy, doxorubicin showed various side effects and induced resistancy of breast cancer cells. Development of targeted therapy on breast cancer focused on combinatorial therapy of doxorubicin and molecular targeted agents. PGV-0 and PGV-1, a curcumin analogue showed potency as co-chemotherapeutic agent with doxorubicin. Our previous study of PGV-0 and PGV-1 showed cytotoxic activity in T47D cells. Therefore, the aim of this research is to examine the synergistic effect of PGV-0, PGV-1 on the cytotoxic activity of doxorubicin through cell cycle modulation and apoptotic induction on MCF-7 breast cancer cell lines. The cytotoxic assay of PGV-0, PGV-1, doxorubicin, and their combination were carried out by using MTT assay. Cell cycle distribution and apoptosis were determined by flowcytometer FACS-Calibur and the flowcytometry data was analyzed using Cell Quest program. Single treatment of PGV-0, PGV-1 and doxorubicin showed cytotoxic effect on MCF-7 with cell viability IC50 value 50 µM, 6 µM and 350 nM respectively. Single treatment of Doxorubicin 175 nM induced G2/M arrest. Single treatment of PGV-0 5 µM induced G2/M arrest while in higher dose 12.5 µM, PGV-0 induced apoptosis. Combination of doxorubicin 175 nM and PGV-0 5 µM induced apoptosis. Combination of doxorubicin 175 nM and PGV-0 12.5 µM also increased apoptosis induction. Single treatment of PGV-1 0.6 µM induced G1 arrest while in higher dose 1.5 µM, PGV-1 induced apoptosis. Combination of doxorubicin 175 nM and PGV-1 0.6 µM induced apoptosis. Combination of doxorubicin 175 nM and PGV-0 1.5 µM also increased apoptosis induction. PGV-0 and PGV-1 are potential to be delevoped as co-chemotherapeutic agent for breast cancer by inducing apoptosis and cell cycle modulation, but the molecular mechanism need to be explored detail. Key words: PGV-0, PGV-1, doxorubicin, co-chemotherapy, breast cancer, cell cycle arrest, apoptosis

ABSTRAK Tikus sawah (Rattus argentivenler Robinson dan Kloss) merupakan jenis hewan mammal yang menimbulkan kerugian besar di sektor pertanian. Selain daya adaptasi yang tinggi terhadap lingkungan, tikus sawah memiliki kemampuan reproduksi yang tinggi dalam waktu singkat, sehingga populasi tikus sawah dapat meningkat dengan cepat Untuk mengatasi hal ini, diperlukan suatu teknik pengendalian, dengan mempengaruhi tingkat fertilitasnya. Biji jarak (Ricinus communis L.) diduga mempunyai efek antifertilitas yang diharapkan dapat mengurangi jumlah populasi tikus sawah. Tujuan penelitian ini adalah untuk mempelajari pengaruh ekstrak biji jarak terhadap struktur mikroskopis testis tikus sawah. Tiga puluh ekor tikus sawah jantan dengan rata-rata berat badan 99,4 g dibagi menjadi 6 kelompok, masing-masing 5 ekor. Satu kelompok kontrol diperlakukan dengan akuabides, 5 kelompok lainnya masing-masing diperlakukan dengan ekstrak etanol biji jarak secara oral dengan metode one way single dose 0.2 ml: 0.4 ml: 0.6 ml: 0.8 ml dan 1.0 ml/100 g bb. Setelah perlakuan tikus sawah dipelihara selama 12 hari. Kemudian tikus dikorbankan dan diambil testisnya untuk dibuat preparat dengan metode parafin, fiksasi dalam larutan Bouin, tebal sayatan 6 um, pewarnaan dengan hematoksilin dan Eosin (HE). Hasil penelitian menunjukkan struktur mikroskopis testis mengalami degenerasi. Uji statistik menunjukkan adanya penurunan secara nyata jumlah spermatogonia, spermatosit dan spermatid testis tikus sawah antara kelompok kontrol dan perlakuan. Makin tinggi dosis, makin tinggi tingkat kerusakan struktur mikroskopis dan jumlah spermatogonia, spermatosit dan spermatid makin sedikit. ABSTRACT Rice-field rat (Rattus argentiventer Robinson & Kloss) is a mammal pest that causes serious lost on agriculture crop. Beside high adaptation, rice-field rats also have high reproduction ability in a short time, so that the population of the rats increase rapidly. To control population of rice-field rats, an operation technology was required by influencing their fertility level. Castor-bean (Ricinus communis L.) seed have antifertility effect expected to reduce the number of rice-field rats population. This research was to learn the effect of castor-bean seeds ethanol extract on microscopic structure of testis rice-field rat. Thirty male rice-field rats with body weight average 99.4 g, were devided into six groups of five. The first group as control was treated with aquabidest and the other five group were treated with 0.2 ml; 0.4 ml; 0.6 ml; 0.8 ml and 1.0 ml per 100 g body weight castor-bean ethanol extract orally respectively with the method of one way single dose. After treatment, rats were kept during 12 days. Thereafter, the experimental rats were sacrificed and incised to remove the testis for routine histological process. The result revealed that testis shown degenerative structure. Statistically, the number of spermatogonia, spermatocytes and spermatids of rice-field rat were decrease compared to control. The higher dose, the higher damage of microscopic structure of testis and the number of spermatogonia, spermatocytes and spermatids were decrease.