Universidad del Sureste

Meningioma is common intracranial benign tumour, but may be diagnosed only at autopsy in developing countries. We present a 38-year-old woman with progressively worsening headache of 7 years duration, who had sudden onset of inability to walk, vomiting and loss of consciousness. She died shortly on admission at the Tamale Teaching Hospital emergency unit. Autopsy conducted found an encapsulated left cerebral hemisphere convexity tumour that had eroded the skull with extension onto the scalp as a swelling. Histological examination confined the tumour to WHO grade I, meningioma.

Integration of the HIV-1 cDNA into the human genome is catalyzed by the viral integrase (IN) protein. Several studies have shown the importance of cellular cofactors that interact with integrase and affect viral integration and infectivity. In this study, we produced a stable complex between HIV-1 integrase, viral U5 DNA, the cellular cofactor LEDGF/p75 and the integrase binding domain of INI1 (INI1-IBD), a subunit of the SWI/SNF chromatin remodeling factor. The stoichiometry of the IN/LEDGF/INI1-IBD/DNA complex components was found to be 4/2/2/2 by mass spectrometry and Fluorescence Correlation Spectroscopy. Functional assays showed that INI1-IBD inhibits the 3′ processing reaction but does not interfere with specific viral DNA binding. Integration assays demonstrate that INI1-IBD decreases the amount of integration events but inhibits by-product formation such as donor/donor or linear full site integration molecules. Cryo-electron microscopy locates INI1-IBD within the cellular DNA binding site of the IN/LEDGF complex, constraining the highly flexible integrase in a stable conformation. Taken together, our results suggest that INI1 could stabilize the PIC in the host cell, by maintaining integrase in a stable constrained conformation which prevents non-specific interactions and auto integration on the route to its integration site within nucleosomes, while LEDGF organizes and stabilizes an active integrase tetramer suitable for specific vDNA integration. Moreover, our results provide the basis for a novel type of integrase inhibitor (conformational inhibitor) representing a potential new strategy for use in human therapy.

The current article criticizes the medical diagnostic construction of the Attention-Deficit/Hyperactivity Disorder, pointing out that this discourse relies on an ideal deficit presented nowadays as a hegemonic point of view. Due to it, the present article proposes a return to the main clinical characteristics arisen by the above mentioned field with the aim to discuss it with the regard of the psychoanalyses' conceptual theory. A child's diagnostic evaluation is presented. Our hypothesis that the difference in children with a diagnosis of Attention-Deficit/Hyperactivity Disorder, goes in the direction of a non-decided structural organization; which surrounds the clinical structures - neuroses, psychoses and perversion - ; indicating a bordering subjective position, on the limit of such structures.