University of Tübingen

There are livestock species such as rabbit, guinea fowl, and grasscutter in Sub-Saharan Africa which are traditionally consumed but have not received as much attention and support from policy and research compared to conventional livestock species such as cattle, sheep, goat, pig and poultry. Despite the growing interest in such neglected livestock species (NLS), evidence on their integration, production, and drivers of adoption remain sparse. This comparative study investigates the production characteristics, profitability, and determinants of farmers' choices between conventional and NLS production in Ogun and Oyo States, Nigeria. We conducted a quantitative survey of 183 smallholder livestock producers, selected using simple random and snowball sampling procedures across Ogun and Oyo States. Data were collected through questionnaires and analysed using descriptive statistics, logistic regression, and cost-benefit analysis. The findings show that women were disproportionately engaged in NLS production. Intensive and semi-intensive production systems were the most prevalent, driven by land scarcity and the adaptability of NLS to confined production environments. Feed emerged as the dominant cost component across both species, significantly impacting profitability. Logistic regression analysis showed that the choice of NLS production was positively and significantly associated with mode of land acquisition, profit, and access to credit. Profitability analysis revealed that NLS systems achieved a higher return on investment than conventional systems, offering a viable pathway for enhancing income, food security, and resilience among smallholders in the study areas. Conclusively, with an appropriate policy framework, neglected livestock species could contribute to a transformative shift in Southwestern Nigeria's livestock landscape.

The central importance of neutrophils for human health is clearly demonstrated by the clinical presentation of patients with severe congenital neutropenia (CN) and cyclic neutropenia (CyN). The majority of patients with CN with neutropenia as their only symptom do not survive the first year of life unless they are treated with recombinant human cytokine granulocyte colony-stimulating factor (rhG-CSF) or undergo allogeneic bone marrow transplantation. Understanding the processes of defective granulopoiesis downstream of CN-associated gene mutations sheds light on the mechanisms that regulate granulopoiesis and granulocyte functions. Furthermore, given that CN is a preleukemia syndrome, studying CN can help to define the role of defective granulopoiesis and deregulated neutrophil functions in leukemogenesis. The development of advanced experimental models of CN and CyN overcomes the limitations of insufficient patient material for research, leading to breakthroughs in the understanding of their pathogenesis and the development of new potential therapies.

Mastering emotion regulation is crucial for social skills and mental health. Hormonal fluctuations, particularly in estradiol (E2) levels across the menstrual cycle, significantly impact emotion processing. E2 is known to influence emotion regulation, mental health, and the plasticity of limbic and striatal regions, which are involved in emotion processing and are rich in E2 receptors. Although research indicates that E2 levels may impact gray matter volume (GMV) of limbic and striatal areas, sufficient causal evidence is missing so far. Furthermore, because of the additional fluctuations of progesterone across the menstrual cycle, the sole impact of E2 on brain volume has been difficult to disentangle. To isolate the effects of E2 from other fluctuating sex hormones, we used a randomized, placebo-controlled, double-blind, crossover design and administered oral E2 to 27 naturally cycling females during their early follicular phase (low endogenous sex hormone levels). We analyzed emotion regulation strategies and E2 levels to assess their impact on regional GMV. Our data showed that a rapid increase of E2 was negatively associated with right striatal GMV. Moreover, greater use of reappraisal was associated with reduced GMV of the right striatum. Rapidly increased E2 did not influence GMV of other limbic regions. These results highlight that rapid increases in E2 and individual differences in emotion regulation dynamically modulate GMV. This offers important implications for female mental and brain health associated with hormonal fluctuations. Considering female endocrine profiles improves hormone-informed health care and therefore supports individualized medicine.

Mesoporous bioactive glasses have emerged as advanced biomaterials due to their highly ordered mesoporous structure, large surface area, and enhanced biological reactivity. These properties distinguish them from conventional bioactive glasses and underpin their growing interest in regenerative medicine and dentistry. This review aims to summarise the development, synthesis, structural characteristics, and applications of mesoporous bioactive glasses, with a focus on their translational potential. A narrative review of the literature was conducted using major scientific databases to identify peer-reviewed studies related to the preparation, characterisation, and biomedical and dental applications of mesoporous bioactive glasses. Relevant in vitro and in vivo studies were critically analysed to synthesise current evidence and emerging trends. Mesoporous bioactive glasses exhibit tunable pore sizes, high surface area, and enhanced ion and drug release capabilities, resulting in rapid hydroxyapatite formation and improved cell adhesion and proliferation. Various synthesis approaches, including sol-gel and templating techniques, allow precise control over composition and mesostructure. Reported applications include bone and soft tissue regeneration, drug delivery, wound healing, and antimicrobial therapies. In dentistry, mesoporous bioactive glasses have been explored for enamel remineralisation, periodontal regeneration, dentin hypersensitivity management, orthodontic applications, endodontic therapy, and implant surface modification. Most evidence remains pre-clinical. Mesoporous bioactive glasses have emerged as a promising class of biomaterials for regenerative and dental applications. However, limitations related to mechanical strength, manufacturing scalability, and the lack of clinical studies currently restrict widespread clinical applications. Mesoporous bioactive glasses offer enhanced bioactivity and therapeutic delivery compared with conventional bioactive glasses, highlighting their potential to improve regenerative and restorative dental outcomes once translational challenges are addressed.

Rhodopsin-mediated autosomal dominant retinitis pigmentosa (RHO-adRP) is a progressive inherited retinal degenerative disorder currently lacking effective treatments. A recurrent 3-base pair deletion in the RHO gene, resulting in the loss of isoleucine at codon 255 or 256 (RHO∆I255 or RHO∆I256), has been identified in patients from the United Kingdom, Germany, Belgium, China, and Korea, suggesting a broad geographic distribution. This mutation leads to rhodopsin (RHO) misfolding, its retention in the endoplasmic reticulum (ER), and aggregation with wild-type (WT) RHO, ultimately triggering ER stress and photoreceptor degeneration. These aggregates are primarily cleared via the ER-associated degradation (ERAD) pathway, with valosin-containing protein (VCP) playing a key role in their retrotranslocation and proteasomal degradation. Pharmacological or genetic inhibition of VCP has shown neuroprotective effects in other models of adRP, but the poor aqueous solubility of VCP inhibitors and challenges in retinal drug delivery hinder clinical translation. To overcome these limitations, we evaluated and compared three VCP-targeted therapeutic strategies in Rho∆I255 knock-in mouse retinae: (1) small-molecule inhibitors (ML240, NMS-873) solubilized in DMSO, (2) ML240 encapsulated in monomethoxy-polyethylene glycol (mPEG)-cholane nanoparticles, and (3) small interfering RNA (siRNA) targeting VCP, delivered via magnetic nanoparticles. Neuroprotective effects were assessed in vitro in retinal explants and in vivo following intravitreal injection. Our findings provide the first evidence that VCP inhibition restores RHO trafficking to the outer segments and prevents photoreceptor cell death in the Rho∆I255 model. Among the three approaches, nanocarrier-encapsulated ML240 exhibited superior efficacy, enabling sustained drug delivery and enhanced photoreceptor protection. These results establish a preclinical proof-of-concept for nanocarrier-mediated VCP inhibition as a promising therapeutic strategy for RHO-adRP and potentially other ER-stress-related retinal degenerations.

Liver cirrhosis profoundly alters pharmacokinetics and is frequently associated with medication-related problems. Stage-specific dosing guidance remains inconsistent, and prescribing information often lacks clarity. As a result, treatment decisions rely on individual clinical judgement and may differ between professional groups. This study assessed drug-selection and dose-adjustment practices among hepatologists, clinical pharmacologists, and clinical pharmacists, quantified consensus across Child-Pugh stages, and evaluated alignment with prescribing information as a basis for harmonised dosing guidance. Twelve experts from hepatology, clinical pharmacology, and clinical pharmacy evaluated prioritised drugs for patients with liver cirrhosis across Child-Pugh stages A-C in a structured survey. For each drug, experts indicated whether they would apply no dose adjustment, modify the dose, or avoid administration. Consensus was defined as at least 75% agreement, and interrater agreement was assessed by pairwise percentage agreement. Consensus recommendations were compared with the dosing information provided in each drug's Summary of Product Characteristics. Consensus or strong consensus was achieved for only 20% of 177 recommendations, mainly for no dose adjustment or avoidance. Agreement decreased with disease severity (Child-Pugh A 83%; B 47%; C 41%). Of 36 consensus recommendations, 18 matched the summary of product characteristics, while six diverged from it. Pharmacists and clinical pharmacologists more often recommended dose adjustments, especially in Child-Pugh B and C, whereas hepatologists preferred standard dosing or avoidance. Expert recommendations for dosing in cirrhosis showed marked variability and limited concordance with prescribing information, underscoring the need for harmonised interdisciplinary guidance integrating pharmacokinetic evidence and clinical feasibility.

Inflammation is a key driver of hematopoietic dysfunction in myeloid malignancies, but its role in the context of hypomethylating therapy remains incompletely understood. Although 5-Azacytidine is used posttransplant in high-risk myelodysplastic syndrome (MDS), only 50% of patients show a clinical response. We provide evidence that inherent inflammatory properties of healthy donor CD34+ stem cells exist that are likely to contribute to the "response" seen in MDS patients. These are linked to epigenetic priming of the myeloid niche, resulting in an S100A8/A9-driven inflammatory program that promotes functionality of immature NK cells. Using in vitro differentiation systems, multi-omic profiling, and an S100A9-/- mouse model, we find that 5-AzaC modulates inflammatory transcriptional programs through epigenetic rewiring of upstream regulatory elements. Loss of S100A9 disrupts myeloid differentiation, impairs NK cell maturation, and alters key developmental regulators including CEBPB, JUN, and NFIL3. In vivo, 5-AzaC restores these defects and primes NK cells in a time- and context-dependent manner. Re-analysis of the published Australian MDS/CMML cohort shows that "responders" display increased S100A8/A9 expression together with enhanced IFN-gamma, IL6-JAK-STAT3, and TNF signaling. These findings suggest that inflammatory myeloid programs may serve as predictive biomarkers and therapeutic targets to enhance NK cell-mediated graft-versus-leukemia activity posttransplant.