Ya'an Polytechnic College

This study aims to analyze the risk factors for wound infection in patients after burn plastic surgery and explore corresponding intervention measures to provide evidence for reducing infection rates in clinical practice. A retrospective study was conducted on 320 patients who underwent burn plastic surgery at SanTai County People’s Hospital from January 2022 to December 2023. Patients were divided into an infection group (86 cases) and a noninfection group (234 cases) based on the occurrence of wound infection postoperatively. Data on age, gender, comorbidities (e.g., diabetes, hypertension), burn type, burn area and depth, surgical duration, antibiotic use, postoperative wound healing status, nutritional status, and lifestyle habits were collected and compared between the 2 groups. Univariate analysis was initially used to screen variables associated with infection, followed by a multivariate logistic regression model to identify independent risk factors for wound infection. The overall infection rate was 26.88%. Univariate analysis indicated that diabetes, surgery duration ≥ 200 minutes, third-degree burns, burn area ≥ 40%, use of 2 or more antibiotics, residual wound presence postoperatively, and malnutrition were significantly different between the 2 groups (P < .05). Multivariate analysis further confirmed that the aforementioned factors were independent risk factors for wound infection after burn plastic surgery, with OR values of 3.571, 2.759, 7.092, 6.775, 3.287, 6.125, and 3.229, respectively, all of which were statistically significant (P < .05). The occurrence of postoperative wound infection is closely associated with multiple factors, particularly diabetes, prolonged surgery duration, deep II–III degree burns, extensive tissue damage, use of multiple antibiotics, incomplete wound healing postoperatively, and malnutrition. Comprehensive perioperative assessment and intervention should be strengthened, and individualized preventive strategies targeting high-risk factors should be developed to reduce infection risk and promote wound healing.

Ganoderma lucidum has a long-standing history of use as a medicinal mushroom, with its spore oil (GLSO) extracted from broken cell walls using CO2 supercritical extraction. However, there is a notable scarcity of experimental studies on the protective effects and underlying mechanisms of GLSO on immune function impairment. The present study aims to explore the characteristics that GLSO contributes to protecting immune functions in cyclophosphamide-induced immunocompromised mice through a multi-omics analysis approach. GLSO administration significantly improved serum hemolysin levels, macrophage phagocytosis, and NK cell activity in immunosuppressed mice. Metagenomics, metabolomic, and proteomic analyses revealed that the immune protection mediated by GLSO was associated with structural rearrangements within gut microflora and shifts in microbial diversity. Specifically, there was an increase in beneficial microorganisms and a decrease in pathogenic organisms, accompanied by various alterations in metabolites and protein expressions. The identified 5 metabolites (propionic acid, beta-glycyrrhetinic acid, 3-aminosalicylic acid, creatine, and 2-phenylacetamide) and 5 proteins (Slc9a9, Blm, Hk3, AP1M2, and J chain) might serve as potential mediators of GLSO to alleviate immune dysfunction collectively caused by CYP in immunosuppressed mice.

ABSTRACT This study presents a comprehensive characterization of the low‐efficiency reservoirs within the Chang 6 member of the Ordos Basin, elucidating their formation mechanisms and establishing quantitative identification standards. These reservoirs typically develop in low‐energy sedimentary microfacies, specifically the margins of underwater distributary channels and inter‐distributary bays. Petrological analysis reveals that the reservoirs consist of fine‐grained feldspar sandstones rich in plastic interstitial materials and carbonate cements (&gt;5%), exhibiting ultralow physical properties (average porosity 7.12%, average permeability 0.27 mD) and a restricted pore structure (average displacement pressure 1.79 MPa, median throat radius 0.03 µm). The genesis of these low‐efficiency zones is governed by a sedimentary‐diagenetic coupling mechanism: Fine‐grained sedimentation facilitated intense mechanical compaction, whereas late‐stage carbonate cementation caused substantial porosity loss, solidifying the tight nature of the formation. Hydrocarbon accumulation follows a “densification before accumulation” model, driven by an overpressure difference of 6–7 MPa generated by the underlying Chang 7 source rocks, which facilitated migration through microfractures into the tight sand bodies. To address the challenge of identifying thin, low‐resistivity oil layers, Walsh inversion and resolution matching techniques were employed to enhance logging signal precision. On the basis of core‐log calibration, a quantitative identification standard was established, defining low‐efficiency oil layers by an acoustic transit time and deep resistivity . Despite their marginal petrophysical properties, the reservoirs exhibit a high brittleness index (&gt;60%) and low horizontal stress difference, indicating that large‐scale volume fracturing is a viable strategy for commercial development.

Endometriosis (EM) is an inflammatory condition that affects approximately 10% of the female-born population. It is characterized by the presence of endometrial tissue outside the uterine cavity, leading to chronic pelvic pain, dysmenorrhea, infertility, and a significant reduction in quality of life. The molecular feature of endometriosis remains poorly understood. Endometriosis-related transcriptomic datasets from the Gene Expression Omnibus (GEO) were normalized and analyzed, and candidate hub genes were identified using two machine learning algorithms. These protein expressions were further validated using proteomic data derived from clinical ectopic and eutopic endometrial tissue samples. Functional assays, including gene silencing and cell migration experiments, were conducted to investigate their biological roles. Glutathione Peroxidase 3 (GPX3) emerged as a candidate diagnostic protein. Silencing GPX3 significantly reduced the migratory capacity of endometriosis cells, likely through modulation of antioxidant activity. In clinical samples, ectopic lesions with high GPX3 expression exhibited increased immune cell infiltration, particularly showing elevated CD68⁺ macrophages and PD-1-positive T cells. Collectively, our findings suggest that GPX3 serves as a potential biomarker for the diagnosis and prognosis of endometriosis, with its expression positively correlating with immune cell infiltration in ectopic endometrial tissue.

High-performance organic solar cells (OSCs) require precise control of the active layer morphology, as it critically governs exciton dissociation and charge transport. Solid additives provide an effective strategy to optimize...

Staphylococcus aureus is a key pathogen in bovine mastitis, and antibiotic therapy is challenged by resistance and residue concerns. Milk-derived extracellular vesicles emerge as promising natural antimicrobials. This study aimed to evaluate the antimicrobial activity and explore potential associated mechanisms of milk-derived extracellular vesicles against S. aureus. Milk-derived EV-enriched fractions (mEVs) from healthy (HmEVs) and mastitic (MmEVs) bovine milk suppressed S. aureus growth in vitro and were associated with oxidative imbalance, with MmEVs showing stronger inhibition. In addition, MmEVs significantly reduced biofilm biomass, extracellular matrix production, and the expression of key biofilm-associated genes (sarA, icaB, fnbA, clfB, cidA). Small RNA sequencing revealed distinct miRNA profiles between HmEVs and MmEVs; in particular, MmEVs were enriched in miRNAs predicted to target the S. aureus biofilm-associated gene clfB. Although we did not directly demonstrate uptake of mEV-derived miRNAs by bacteria or their regulation of bacterial gene expression in this study, our small RNA sequencing data together with subsequent bioinformatic predictions suggest that vesicular miRNAs should be regarded as candidate contributors, rather than demonstrated mediators, of the observed antibacterial and antibiofilm effects. Taken together, these findings indicate the potential of mEVs as residue-free adjuncts for controlling bovine mastitis, while recognizing that the present conclusions are mainly derived from in vitro experiments with S. aureus and bioinformatic analyses. Therefore, functional validation of candidate miRNAs, in vivo studies, and evaluation of activity against other mastitis-associated pathogens are still required to clarify the underlying mechanisms, therapeutic potential, and spectrum of activity of mEVs.