참깻묵이 거세 한우의 비육성적과 등심의 지방산 조성, 유리 아미노산 함량 및 관능평가에 미치는 영향

Obstructive jaundice is associated with high morbidity and mortality. Major complications such as pulmonary dysfunction, renal failure and sepsis are frequently encountered. Recent studies and observations suggest that the free oxygen radicals (FORs) produced in obstructive jaundice may play a significant role in the etiopathogenesis of acute renal failure (ARF). Thirty rats were divided into three groups, as sham, control and treatment groups containing 10 rats each. Laparatomy was performed on each animal in the control and treatment groups and common bile ducts were ligated. Common bile duct was observed but was not ligated for the rats in the sham group. Saline solution injection was begun on the first day of surgical procedure and repeated once a day during the following 5 days. The same procedure was performed with oxygen radical scavenger dimethyl sulfoxide (1.5 mg/kg/day i.p.) instead of saline in the treatment group. The rats were sacrificed on the 7th postoperative day. On the 7th postoperative day, the bilirubin, urea and creatinine levels of the control and treatment groups were significantly higher in comparison with the sham group (p < 0.01). Although there was no statistically significant difference between the bilirubin levels of the control and treatment groups (p > 0.05), the urea and creatinine levels in the treatment group were significantly lower (p < 0.01). On the 7th postoperative day, the erythrocyte superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) levels of the control and treatment groups were significantly lower than those of the sham group (p < 0.01), whereas renal and erythrocyte malondialdehyde (MDA) levels were significantly higher (p < 0.01). Although SOD and GSH-Px levels did not differ significantly between the treatment and control groups (p > 0.05), renal and erythrocyte MDA levels of the treatment group were significantly lower than those of the control group (p < 0.01). The histopathological scores were significantly higher in the control and treatment groups (p < 0.01); there was no significant difference between the control and treatment groups (p > 0.05). FORs seem to play a significant role in the etiopathogenesis of renal failure in obstructive jaundice. Antioxidant treatment may decrease oxidative damage due to FORs and may prevent renal failure.

본 연구는 비육한우 32두(거세우 16두, 암소 16두)를 대상으로(C-1구 거세우에게 일반사료 : C-2구 암소에게 일반사료 : T-1구 거세우에게 쑥 2% 첨가 : T-2구 암소에게 쑥 2% 첨가) 4개 처리구를 두고 14개월간(생후 14개월-27개월) 비육시험한 결과는 다음과 같다. 등심부위의 전단력과 보수성은 T-1구(<TEX>$3.20kg/0.5inch^2$</TEX>, 54.32%)가, 가열감량은 C-2구(22.22%)가 다른 처리구보다 크게 높았다(p<0.05). 관능검사의 다즙성, 연도 및 향미는 처리구가 대조구보다 높은 경향을 보였고, 그 중에서도 T-1구(5.50점-5.20점)가 가장 높게 나타났다. Catechin 성분에서 총 카테킨 및 에피카테킨 성분은 처리구(0.43-0.46 mg/kg, 0.056-0.065 mg/kg)가 대조구(0.23-0.25 mg/kg, 0.031-0.032 mg/kg)보다 더 높게 나타났다. 지방산 조성에서 oleic acid은 47.56-48.45% 정도, palmitic acid은 28.86-29.67%, stearic acid는 9.92-10.82%, palmitoleic acid 및 myristic acid는 4.68-5.49%와 2.48-3.89%의 범위로서 처리간에 유의차가 없었다. 이상의 시험결과로 볼 때, 거세한우에 대한 쑥첨가는 육질의 관능검사(다즙성, 연도, 향미) 및 이화학적 특성(전단력, 보수성)에서 크게 향상되었다. This study was conducted to determine the effect of feeding dietary mugwort on the beef quality during 14 month(from 14 month to 27 month) with 32 heads of fattening Hanwoo. Fattening Hanwoos were randomly allotted 4 treatments(8 heads/treatment): C-1[(control(0%) of Hanwoo steers)], C-2[(control(0%) of Hanwoo heifers)], T-1(supplemented with mugwort powder 2.0% of Hanwoo steers), T-2(supplemented with mugwort powder 2.0% of Hanwoo heifers). The shear force value and water holding capacity(WHC) of longissimus muscle were the highest in T-1(<TEX>$3.20kg/0.5inch^2$</TEX>, 54.32%) and cookingloss was in C-2(22.22%) than the others(p<0.05). However, panel test scores of juiciness, tenderness and flavor of longissimus muscle were the highest for T-1(5.20-5.50 point) between the treatment group. The total catechin and epicatechen in longissimus muscle were higher in treatment group(0.43-046 mg/kg, 0.056-0.065 mg/kg) than control group(0.23-0.25 mg/kg, 0.031-0.032 mg/kg)(p<0.05). The content of total fatty acid in longissimus muscle were oleic acid(48.45-47.56%), palmitic acid(29.67-28.86%), stearic acid(9.92-10.82%), palmitoleic acid and myristic acid(4.68-5.49%, 2.48-3.89%) of range. In conclusion, the effects of feeding dietary mugwort of Hanwoo steer were improved the quality beef with highly panel test scores(juiciness, tenderness, flavor) and physical-chemical characteristics(shear force value, WHC).

Objective This study aimed to evaluate the effectiveness and safety of Fuzheng Xiaoji granule in patients with stage IIIC colorectal cancer. Methods A total of 150 patients with stage IIIC colorectal cancer treated in Shanghai Ruijin Hospital from January 2019 to January 2022 were selected. They were divided into treatment and control groups according to a 2 : 1 random number table. There were 100 cases in the treatment group and 50 cases in the control group. The treatment group was administered Fuzheng Xiaoji (FZXJ) granule, and the control group was administered the placebo orally. The primary endpoint was disease-free survival (DFS). In addition, after 6 months, the changes in Traditional Chinese Medicine (TCM) symptom score (fatigue, emotional depression, chest tightness, insomnia, anorexia, abdominal distension, abdominal pain, soreness and weakness in the waist and legs, chills, and dysphoria in the chest, palm, and soles) were compared. Results The DFS was 34.37 ± 2.91 months in the control group and 37.0 ± 1.08 months in the treatment group (p < 0.05). Compared with the control group, the treatment group showed less fatigue, abdominal distension, and soreness and weakness in the waist and legs (p < 0.05), significantly. The scores of emotional depression and anorexia decreased obviously, with a significant difference between the control and treatment groups (p < 0.01). There were no significant differences between the control and treatment groups in the incidence of chest tightness, insomnia, abdominal pain, chills, and dysphoria in the chest, palm, and soles (p > 0.05). Conclusion Fuzheng Xiaoji granule can improve patients' symptoms and prolong the DFS.

The effects of probiotic administration were studied in an experiment, using 120 newborn calves. Calves were randomly assigned to two experimental groups (sixty calves for each group) and probiotic (0.25 g h(-1) day(-1)) was added in their daily milk intake until 90 days of age. After the first week, all calves (including control group) received starter ration containing 21.0% crude protein and 3.0% crude fat. Body weight gain, body height and general health condition of all calves were observed at day 30, 60 and 90. Also condition of feces was examined daily and the occurrence of diarrhea was recorded throughout the experiment. Mean values of weight gain during three successive months for treatment and control groups were 57.52 and 50.58 kg, respectively. Body weight gained was not significantly different for first and second mo between treatment and control groups (16.9 and 33.87 vs. 14.49 and 33.07 for fist and second months in treatment and control groups, respectively). However, these values were significantly different (p < 0.001) between treatment (57.52) and control (50.58) groups third months of age. Diarrhea was observed in 35 calves of control group, which was higher than 11 cases in calves treated with probiotic (p < 0.001). The body height values of control and treatment groups in three successive months were 5.49, 10.82 and 15.00 cm for control and 5.44, 9.25 and 15.75 cm for treatment groups in first, second and third mo, respectively, which showed no significant difference between two groups during this study. The results of this study indicated that present probiotic compound have beneficial effects, especially on the third month of age in rearing calves.

Laparoscopy approach to cholecystectomy has shortened the recovery period, reducing discharge times from 1 to 3 days to same-day discharge. We hypothesize that the use of more than one modality to prevent postoperative pain may be more efficacious than single modality. Patients were randomized to a treatment (n = 24) or control (n = 25) group and studied using a prospective, double-blind design. Preoperatively, at 45 min before induction of anesthesia, the treatment group received an intramuscular (IM) bolus injection of meperidine 0.6 mg/kg and ketorolac 0.5 mg/kg. The control group received two bolus IM injections of placebo (normal saline). Ten minutes before incision, local anesthesia (treatment group) or saline (control group) was infiltrated into the skin of each patient. Anesthetic management, postoperative pain, and nausea treatment were standardized. Pain and nausea assessment were done 1 h preoperatively, 0, 0.5, 1, 2, 3, and 4 h postoperatively, at discharge, and 10, 24, and 48 h postoperatively. Patients were discharged by scoring criteria. Postoperatively, significantly more patients in the treatment group were without pain on arrival in the postanesthesia care unit (PACU), 12/21 (57.1%) vs 1/24 (4.2%) in the control group (P < 0.001). Similarly, the severity of pain was sixfold less in the treatment group than in the control group. The incidence of nausea in the PACU was significantly less in the treatment group; 4.7% vs 29.5% in the control group (P < 0.05). Patients from the treatment group satisfied Postanesthesia Discharge Score significantly earlier than those in the control group (281 +/- 12 min vs 375 +/- 19 min; P < 005). The concomitant use of local anesthetic and nonsteroidal antiinflammatory and opioid drugs proved to be highly effective in our patients, resulting in faster recovery and discharge. (Anesth Analg 1996;82:44-51)

To find out the effect of heat stress alleviation on physiological response, metabolic hormone profiles, milk production and composition in lactating buffaloes during hot-dry (HD) and hot-humid (HH) seasons, 42 lactating Nili-Ravi buffaloes were uniformly divided into 2 groups of 21 each. The treatment (T1) group was supplemented with niacin, yeast, edible oil and provided with curtains, additional ceiling fans and mist fans in the shed. The feeding time, frequency and method for providing concentrate were altered. The control (T0) group buffaloes were kept in separate shed without any nutrient supplementation, modification in microclimate and management. The rectal temperatures, recorded at 10 AM and 3 PM, were significantly lower in treatment group buffaloes than control group under both seasons. Similar trends in pulse rate and respiration rate were recorded among treatment and control group buffaloes in both seasons. Plasma cortisol, T3 and T4 values were almost similar in both control and treatment groups. The average daily milk production was significantly higher in treatment group than control group from starting to the end of the experiment. The average total fat and SNF production in treatment group were significantly higher compared to control group. The overall fat per cent in milk of control group buffaloes was recorded to be 7.71% which was significantly higher than treatment group. It was concluded that nutrient supplementations, microclimate modifications and management alterations together in the form of one package help reducing heat stress.It has beneficial effect on physiological responses, total milk production and composition in lactating buffaloes during hot dry and hot humid summer without affecting cortisol, T3 and T4 profile.

The aims of the present study were to investigate the effect of altrenogest treatment from 109 to 112 days of gestation in combination with double intramuscularly�of PGF2alpha administrations�at 113 days of gestation on the variation of farrowing in sows and its side effects on the sow colostrum yield, colostrum IgG, newborn piglet characteristics and piglet survival rate until seven days of postnatal life. In total, 193�sows were randomly allocated according to parity number into two groups, i.e. control (n = 95) and treatment (n = 98). The control sows were allowed to farrow naturally. The treatment sows were orally administered 20 mg per day of altrenogest for 4 days from 109 to 112 days of gestation and were administered PGF2alpha twice on day 113 of gestation. Individual body weight at birth and at 24 h after birth of piglets in all the litters were determined in both control (n = 1,609) and treatment (n = 1,707) groups. Colostrum consumption of all the piglets and colostrum yield of sows were estimated. Colostrum IgG at 0, 6 and 24 h postpartum and serum progesterone before and after farrowing were determined. Piglet survival rate until 3 days and 7 days of postnatal life were evaluated. On average, total number of piglets born and number of piglets born alive per litter were 17.0 ? 3.1 and 15.4 ? 3.0, respectively. The proportion of sows farrowed before 114 days of gestation in control was higher than treatment groups (8.4% and 2.0%, respectively, P = 0.05) and 92.8% of sows in the treatment group farrow on day 114 of gestation. The proportion of sows farrowed during working hours in control was lower than treatment groups (50.5% and 65.3%, respectively, P = 0.038). The percentage of stillborn piglets per litter did not differ significantly between in control and treatment groups (4.5% and 4.6%, respectively). Colostrum yield of sows did not differ between treatment and control group (5.28 ? 0.12 kg�and 5.52 ? 0.13 kg, respectively, P = 0.174). However, colostrum intake of piglets in the treatment was lower than control groups (357.0�? 6.6 g and 381.2 ? 7.0 g, respectively, P = 0.012). Colostrum IgG in the control was higher than treatment groups (41.2 ? 1.1 and 37.3 ? 1.0 mg per ml, respectively, P = 0.013). In conclusion, altrenogest treatment from 109 to 112 days and double PGF2alpha administrations on day 113 of gestation can reduce early parturition and can control gestation length in sows. No deleterious effect of this protocol on either the incidence of stillbirths or sow colostrum yield were detected. However, the piglet colostrum intake and colostrum IgG in the treatment groups were lower than control. Thus, intensive care of newborn piglets in the treatment group should be considered.

Abomasal hypomotility and a decreased rate of abomasal emptying are believed to play important roles in the etiopathogenesis of abomasal disorders in ruminants. The abomasal emptying rate is potentially influenced by several factors. Effect of endotoxins on contractility of smooth-muscle preparations from the bovine abomasal antrum has been evaluated, and the results showed that endotoxemia has an inhibitory effect on contractility of abomasal smooth-muscle fibers. To evaluate the effect of the endotoxemia on the abomasal emptying rate in suckling lambs, this study was conducted. This study was done on five suckling lambs in two phases. In phase 1 (control group), the first blood sample was obtained in 7 a.m. (time zero). Then a mixture of milk, barium sulfate 70%, and acetaminophen was administered orally to each lamb. Afterwards, blood samples were obtained each 15 min up to 4 h later. Phase 2 (treatment group) was similar to phase 1, but after the first blood sampling in time zero in 7 a.m., endotoxemia was induced by administration of commercial lipopolysaccharide at dose rate of 2 μg/kg. The rest of the processes of phase 2 were similar to phase 1. Cmax, Tmax, and area under concentration curve (AUC) of acetaminophen were measured in the control and treatment groups. The abomasal emptying rate was assessed by comparing Cmax, Tmax, and AUC of acetaminophen between the control and treatment groups. The data from the control and treatment groups were analyzed statistically by SPSS program, using one-way ANOVA. Results indicated that Cmax, Tmax, and AUC in the endotoxemic (treatment) group were significantly different compared with those in the control group, which means endotoxemia decreased abomasal emptying rate. Results of present study indicated that endotoxemia profoundly decreases the abomasal emptying rate in suckling lambs. Thus, administration of drugs which attenuate endotoxemia could enhance the abomasal emptying rate in endotoxemic animals.

Objective To evaluate the therapeutic effect of Kangbingdu oral liquid in combination with ribavirin aerosol for hand, foot, and mouth disease （HFMD）. Methods A total of 135 HFMD children were enrolled and assigned to receive ribavirin aerosol alone （ontrol group, 67 patients） or ribavirin aerosol plus Kangbingdu oral liquid group （treatment group, 68 patients） for 7 days. Therapeutic effect was evaluated as the time to fever resolution, subsidence of skin rash and oral ulcers. All throat swab samples were tested using a fluorescence quantitative RT-PCR assay for panenteroviruses, enterovirus 71（EV71）and Coxsackievirus A16 （CA16）. Results The time to fever resolution（2.51±0.42 d vs. 3.92±0.61 d;t=15.621, P<0.01）, subsidence of skin rash（4.22±1.34 d vs. 6.33±1.41 d;t=8.914, P<0.01）and oral ulcers（3.01±0.32 d vs. 4.52±1.21 d;t=9.880, P<0.01）in the treatment group were significantly shorter than those in the control group. The healing rate in the treatment group were significantly higher than that in the control group（88.2%vs. 44.8%;χ^226.761, P<0.01）, and total effective rate was no different in treatment and control groups（94.1%vs. 83.6%;χ^22.801, P=0.094）. The rates of conversion from positive to negative for panenteroviruses（90.9% vs. 52.6%;χ^213.331, P<0.01）and EV71（83.3%vs. 52.4%;χ^24.307, P=0.038）in the treatment group were significantly higher than those in the control group, and the rate of conversion from positive to negative for CA16 was no different in treatment and control groups（93.3%vs. 81.8%;χ^20.082, P=0.774）. Conclusion Kangbingdu oral liquid in combination with ribavirin aerosol may improve the rate of conversion from positive to negative for enteroviruses, reduced the time to fever resolution, subsidence of skin rash and oral ulcers in HFMD children. Key words: Hand,foot and mouth disease; Ribavirin; Kangbingdu oral liquid; Child

Suppression of Neovascularization by Topical and Subconjunctival Bevacizumab After High-Risk Corneal Transplantation

Efficacy and safety of Qingfei Huatan formula in the treatment of acute exacerbation of chronic obstructive pulmonary disease: A multi-centre, randomised, double-blind, placebo-controlled trial

To determine effectiveness of advice from general practitioners to heavy drinkers to reduce their excessive alcohol consumption (35 U or more a week for men, 21 U or more for women). Randomised, controlled double blind trial over 12 months with interim assessment at six months. Group practices (n = 47; list size averaging 10,000) recruited from Medical Research Council's general practice research framework, mostly in rural or small urban settings. Patients recruited after questionnaire survey. Of total of 2571 (61.2%) of 4203 patients invited for interview who attended, 909 (35.4%) stated that in past seven days they had drunk above the limits set for study and had not received advice; they were randomised to control and treatment groups. Patients in treatment group were interviewed by general practitioner (who had had a training session) and received advice and information about how to reduce consumption and also given a drinking diary. Study aimed at detecting a reduction in proportion of men with excessive alcohol consumption of 30% in treatment group and 20% in control group (for women 40% and 20%, respectively) with a power of 90% at 5% level of significance. In addition, corroborative measures such as estimation of gamma-glutamyltransferase activity were included. At one year a mean reduction in consumption of alcohol of 18.2 (SE 1.5) U/week had occurred in treated men compared with a reduction of 8.1 (1.6) U/week in controls (p less than 0.001). The proportion of men with excessive consumption at interview had dropped by 43.7% in the treatment group compared with 25.5% in controls (p less than 0.001). A mean reduction in weekly consumption of 11.5 (1.6) U occurred in treated women compared with 6.3 (2.0) U in controls (p less than 0.05), with proportionate reductions of excessive drinkers in treatment and control groups of 47.7% and 29.2% respectively. Reduction in consumption increased significantly with number of general practitioner interventions. At one year the mean value for gamma-glutamyltransferase activity had dropped significantly more in treated men (-2.4 (0.9)IU/l) than in controls (+1.1(1.0)IU/l; t = 2.7, p less than 0.01). Reduction in gamma-glutamyltransferase activity tended to increase with number of intervention sessions in men. Changes in gamma-glutamyltransferase activity in women and changes in other indicators in both sexes did not differ significantly between treatment and control groups. If the results of this study were applied to the United Kingdom intervention by general practitioners could each year reduce to moderate levels the alcohol consumption of some 250000 men and 67500 women who currently drink to excess. General practitioners and other members of the primary health care team should therefore be encouraged to include counselling about alcohol consumption in their preventive activities.

Objective The purposes of the present study are to investigate the effects and mechanisms of Bu Shen Ning Xin Yi Jing Pill on premature ovarian failure (POF) through a clinical study. Methods Sixty women with POF were administered and randomized into the treatment group and the control group. Every group had thirty cases. Patients in the treatment and control groups were requested to have the Bu Shen Ning Xin Yi Jing Pill and the Liu Wei Di Huang Pill, respectively. Six-month duration was considered as a therapeutic course. The serum levels of follicle stimulating hormone (FSH) and estradiol (E2) were determined by the chemistry luminescence method. Differences between the treatment and control groups were investigated.Results Improved menstruation were revealed in both groups and the treatment group showed significant improvement than the control group (P<0.01). In the treatment group, serum FSH levels sharply dropped after treatment, while the serum E2 levels significantly elevated compared with those of prior-treatment (P<0.01) . The total effective rate in the treatment group was 70% (21 cases), whereas 40% in the control group (12 cases). The difference had statistical significance (P<0.01) .Conclusion The BuShen NingXin YiJing Pill is effective in the treatment of premature ovarian failure via significant improvements in the clinical symptoms and balance of the endocrine hormones. Key words: Premature ovarian failure: Herbal medicine: BuShen NingXin YiJing Pill

This study was conducted to determine if the use of the technology known as Classroom Performance System (CPS), specifically referred to as “Clickers”, improves the learning gains of students enrolled in a biology course for science majors. CPS is one of a group of developing technologies adapted for providing feedback in the classroom using a learner-centered approach. It supports and facilitates discussion among students and between them and teachers, and provides for participation by passive students. Advocates, influenced by constructivist theories, claim increased academic achievement. In science teaching, the results have been mixed, but there is some evidence of improvements in conceptual understanding. The study employed a pretest-posttest, non-equivalent groups experimental design. The sample consisted of 226 participants in six sections of a college biology course at a large community college in South Florida with two instructors trained in the use of clickers. Each instructor randomly selected their sections into CPS (treatment) and non-CPS (control) groups. All participants filled out a survey that included demographic data at the beginning of the semester. The treatment group used clicker questions throughout, with discussions as necessary, whereas the control groups answered the same questions as quizzes, similarly engaging in discussion where necessary. The learning gains were assessed on a pre/post-test basis. The average learning gains, defined as the actual gain divided by the possible gain, were slightly better in the treatment group than in the control group, but the difference was statistically non-significant. An Analysis of Covariance (ANCOVA) statistic with pretest scores as the covariate was conducted to test for significant differences between the treatment and control groups on the posttest. A second ANCOVA was used to determine the significance of differences between the treatment and control groups on the posttest scores, after controlling for sex, GPA, academic status, experience with clickers, and instructional style. The results indicated a small increase in learning gains but these were not statistically significant. The data did not support an increase in learning based on the use of the CPS technology. This study adds to the body of research that questions whether CPS technology merits classroom adaptation.

Objective To examine the inhibitory effect of IL-2-PE40 on the mouse corneal allograft rejection. Methods A mouse corneal graft model was set up by using C57BL/6 mice as donors and Balb/c mice as recipients. In the treatment group, IL-2-PE40 (0.6 μg/g body weight) was intraperitoneally injected from the day of surgery every 12 h until rejection happened. In the control group, the equal volume of PS was injected intraperitoneally at the corresponding time points. The transplanted cornea was observed under slit-lamp twice a week and the transplanted corneal opacity and neovascularization were rated according to Horis grading standards. It led to the determination of rejection response. The survival of transplanted cornea was regarded to be stopped when the rejection occurred. The operated eyes were observed historically on the 10th, 15th, 25th and 35th day after the surgery, and the peripheral blood was collected for measurement of T cell subgroups and T lymphocyte colonies. Results The survival time of cornea in the treatment and control groups was (30.2±2.9) days and (15.1±2.1) days respectively. In the control group, rejection occurred on the 15th day after the surgery, CD4~+ cells started rise right after the surgery and increased most obviously on the 15th day [(63.9±4.0)%] and decreased afterwards. CD4~+ cells in the treatment group were increased slightly [(42.6±4.0)%] on the 15th day. The number of CD4~+ cells in the treatment group was obviously less than in the control group (P<0.01). No significant changes in CD8~+ cells were observed in both groups. The number of T lymphocyte colonies in the control group was increased at the beginning and dropped then. No obvious change was found in the treatment group. The number of T lymphocyte colonies in the treatment group was significantly less than in the control group (P＜0.01). Conclusion IL-2-PE40 is a highly specific immunosuppressive agent. It can delay the development of corneal graft rejection and reduce the percentage of T-helper cells significantly. It also works to weaken the forming capacity of the peripheral T lymphocyte colonies. Key words: Corneal transplantation; immunotoxins; Graft rejection; Mice