АЛГОРИТМ ОЦЕНКИ ГРАФИЧЕСКИХ ПОЛЬЗОВАТЕЛЬСКИХ ИНТЕРФЕЙСОВ ПО ВРЕМЕНИ ПОИСКА ИНФОРМАЦИОННО-ФУНКЦИОНАЛЬНЫХ ОБЪЕКТОВ

The relevance of the research problem is determined by the active development of the digital society, which requires the formation of new forms of civic participation of the generation of the digital age, and Scientific libraries as leading information and communication institutions of society play a significant role in forming the value foundations of the worldview of modern youth as citizens of the digital age. The purpose of the article was to determine the prospects for the implementation of the concept of digital citizenship in scientific libraries. The research methodology was based on a combination of source analysis, comparative analysis, dialectical, systematic and logical methods of scientific research. As a result of the conducted research, it was stated that the representatives of the digital generation in the socio-communication aspect are an independent, critical, socially active community, formed thanks to the active use of the Internet and social media for obtaining information and communication. The characteristic features of the worldview of a “digital person” include fluency in digital and information-analytical technologies, speed of search and perception of new information, short attention span, giving preference to visualized information, brevity of presentation, “clip-like” perception. It is the character of the “digital person” that defines the reader not of the future, but of the present, because the generation of 20-30-year-olds is the first generation that was born in the digital world and can no longer imagine life without the mobile Internet and various gadgets, which necessitates the global transformation of traditional forms of work of libraries in the direction of active use of digital technologies. The work emphasized that university libraries should provide progressive education based on the expansion and deepening of digital competences of users, taking into account moral and ethical, socio-legal and psychological factors. The implementation of the concept of digital citizenship necessitates active digitization of library funds, expansion of the spectrum of virtual products and services, as well as active positioning of the library's activities in social networks. Prospects for further research consist in the expansion and deepening of practical recommendations for the formation of the concept of digital citizenship by means of library practices

Background Children are very curious. They like to play games but lack reading fluency. Conventional interfaces for information-seeking in digital libraries are unsuitable for children because they are rigid, text-based, and task-oriented. Thus, this study developed a customized interface for a digital library. To facilitate children in online searches, the design concepts included user-friendly target icons, icon recognition, and way-finding in a two-dimensional visualized environment. Methods In this study, the criteria used to develop visualization tools that children can use for information retrieval were based on theories of human perception related to graphical interfaces for information visualization systems, including iconic representations and information density on visualized displays. Databases widely used by children in the National Library of Public Information were linked to the suggested database frame. The database frame was classified into five major subject categories: Natural Sciences, Fine Arts and Music, Health and Athletics, History and Culture, and Treasure of Knowledge. The web interface was designed according to way-finding concepts and map-structure hierarchies. Information searches in the main frame, sub-frames, and linked database were designed to be simple and intuitive for use by children. Results Information search efficiency was measured in 104 children assigned to search the virtual environment for icons (databases) that adequately supported their studies. Statistical analyses showed that, compared to the text-lined interface, the graphical interface improved the success and speed of searches by children. Conclusions Incorporating theories of spatial memory and representative icons in designs for visualized retrieval systems helps children to recognize different categories of knowledge content and improves their efficiency in information retrieval. To improve database search efficiency in children, information recommendation issues require further study. However, further research is also needed to identify factors that affect patterns of information-seeking behavior in children.

In this paper, two new and efficient algorithms for block motion estimation are presented. The proposed algorithms concentrate on improvements to existing block motion estimation algorithms (BMAs) and include four effective steps: prediction of static blocks, prediction of optimal search starting point, motion categorization for the block and motion vector search around the optimal starting point. Two different search patterns are presented that achieve different tradeoffs between search speed and reconstructed picture quality. Extensive simulation results and comparative analysis with the well-known Diamond search show that the proposed algorithms outperform conventional algorithms in terms of computational complexity while maintaining picture quality.

Design paradigms often ignore the diverse goals users bring to the computer interface. Any human-computer interaction can be viewed as a marriage of two systems: The user begins the interaction by formulating an information goal, and the computer software meets that goal with a sometimes complex list of potential topic areas. The user then accesses that topic list through the computer interface. Part of the act of accessing the topic list is selecting a potential topic, and this action is often supported by a menu interface. Although research is pervasive on how best to organize menu items to facilitate learning, search speed, and reduced selection errors, little has been done to examine the impact of different types of user goals or cues on a menu's effectiveness. In a study using three distinct cues-direct match, synonym, and iconic - and two menu organizations - alphabetical and functional-data suggest that (a) the functional menu is more effective than the alphabetical menu for the synonym and iconic cues, (b) learning occurs with both menu designs (i.e., selection speed increases rapidly across the five trial blocks), and (c) users make fewer errors with the functionally organized menu. The results, in general, encourage more rigorous investigation of the interaction between the tasks users bring to menu interfaces and the optimal design of those menus.

The replication of DNA is a complex biological process that is essential for life. Bacterial DNA replication is initiated at genomic loci referred to as replication origins (oriCs). Integrating the Z-curve method, DnaA box distribution, and comparative genomic analysis, we developed a web server to predict bacterial oriCs in 2008 called Ori-Finder, which is helpful to clarify the characteristics of bacterial oriCs. The oriCs of hundreds of sequenced bacterial genomes have been annotated in the genome reports using Ori-Finder and the predicted results have been deposited in DoriC, a manually curated database of oriCs. This has facilitated large-scale data mining of functional elements in oriCs and strand-biased analysis. Here, we describe Ori-Finder 2022 with updated prediction framework, interactive visualization module, new analysis module, and user-friendly interface. More species-specific indicator genes and functional elements of oriCs are integrated into the updated framework, which has also been redesigned to predict oriCs in draft genomes. The interactive visualization module displays more genomic information related to oriCs and their functional elements. The analysis module includes regulatory protein annotation, repeat sequence discovery, homologous oriC search, and strand-biased analyses. The redesigned interface provides additional customization options for oriC prediction. Ori-Finder 2022 is freely available at http://tubic.tju.edu.cn/Ori-Finder/ and https://tubic.org/Ori-Finder/.

Breast cosmesis is an important endpoint of breast conserving therapy (BCT), but a gold standard method of its evaluation is not yet established. The St. George and Wollongong Randomised Breast Boost trial used five different methods of cosmetic assessment, including both subjective and objective, to comprehensively evaluate the cosmetic outcome of the trial patients. This current study analyses the level of concordance between these methods in an attempt to determine a possible standard in the evaluation of breast cosmesis. Patients attending follow-up clinic reviews at 5years post breast radiotherapy were evaluated. Patients completed a cosmesis and functional assessment questionnaire, assessing clinicians completed an EORTC (European Organization for Research and Treatment of Cancer) cosmetic rating questionnaire and photographs were obtained. The photographs were later assessed by a panel of five experts, as well as analysed using the objective pBRA (relative Breast Retraction Assessment) and the BCCT.core (Breast Cancer Conservative Treatment.cosmetic results) computer software. Scores were dichotomised to excellent/good and fair/poor. Pairwise comparisons between all methods, except pBRA, were carried out using overall agreement calculations and kappa scores. pBRA scores were compared on a continuous scale with each of the other dichotomised scores obtained by the other four methods. Of 513 St George patients alive at 5years, 385 (75%) attended St George for follow-up and consented to photography. Results showed that assessment by physicians in clinic and patient self-assessment were more favourable regarding overall cosmetic outcome than evaluation of photographs by the panel or the BCCT.core software. Excellent/good scores by clinician-live and patient self-assessments were 93% and 94% respectively (agreement 89%), as compared to 75% and 74% only by BCCT.core and panel assessments respectively (agreement 83%, kappa 0.57). For the pBRA measurements, there was a statistically significant difference (P <0.001) between scores for excellent/good versus fair/poor cosmesis by all four methods. The range of median pBRA measurements for fair/poor scores was 13.4-14.8 and for excellent/good scores was 8.0-9.4. Incorporating both BCCT.core assessment and patient self-assessment could potentially provide the basis of a gold standard method of breast cosmetic evaluation. BCCT.core represents an easy, time efficient, reproducible, cost effective and reliable method, however, it lacks the functional and psychosocial elements of cosmesis that only patient self-reported outcomes can provide.

The 35S ribosomal DNA (rDNA) units, repeated in tandem at one or more chromosomal loci, are separated by an intergenic spacer (IGS) containing functional elements involved in the regulation of transcription of downstream rRNA genes. In the present work, we have compared the IGS molecular organizations in two divergent species of Fagaceae, Fagus sylvatica and Quercus suber, aiming to comprehend the evolution of the IGS sequences within the family. Self- and cross-hybridization FISH was done on representative species of the Fagaceae. The IGS length variability and the methylation level of 18 and 25S rRNA genes were assessed in representatives of three genera of this family: Fagus, Quercus and Castanea. The intergenic spacers in Beech and Cork Oak showed similar overall organizations comprising putative functional elements needed for rRNA gene activity and containing a non-transcribed spacer (NTS), a promoter region, and a 5′-external transcribed spacer. In the NTS: the sub-repeats structure in Beech is more organized than in Cork Oak, sharing some short motifs which results in the lowest sequence similarity of the entire IGS; the AT-rich region differed in both spacers by a GC-rich block inserted in Cork Oak. The 5′-ETS is the region with the higher similarity, having nonetheless different lengths. FISH with the NTS-5′-ETS revealed fainter signals in cross-hybridization in agreement with the divergence between genera. The diversity of IGS lengths revealed variants from ∼2 kb in Fagus, and Quercus up to 5.3 kb in Castanea, and a lack of correlation between the number of variants and the number of rDNA loci in several species. Methylation of 25S Bam HI site was confirmed in all species and detected for the first time in the 18S of Q. suber and Q. faginea. These results provide important clues for the evolutionary trends of the rDNA 25S-18S IGS in the Fagaceae family.

Analyses of genome reduction in obligate bacterial symbionts typically focus on the removal and retention of protein-coding regions, which are subject to ongoing inactivation and deletion. However, these same forces operate on intergenic spacers (IGSs) and affect their contents, maintenance, and rates of evolution. IGSs comprise both non-coding, non-functional regions, including decaying pseudogenes at varying stages of recognizability, as well as functional elements, such as genes for sRNAs and regulatory control elements. The genomes of Buchnera and other small genome symbionts display biased nucleotide compositions and high rates of sequence evolution and contain few recognizable regulatory elements. However, IGS lengths are highly correlated across divergent Buchnera genomes, suggesting the presence of functional elements. To identify functional regions within the IGSs, we sequenced two Buchnera genomes (from aphid species Uroleucon ambrosiae and Acyrthosiphon kondoi) and applied a phylogenetic footprinting approach to alignments of orthologous IGSs from a total of eight Buchnera genomes corresponding to six aphid species. Inclusion of these new genomes allowed comparative analyses at intermediate levels of divergence, enabling the detection of both conserved elements and previously unrecognized pseudogenes. Analyses of these genomes revealed that 232 of 336 IGS alignments over 50 nucleotides in length displayed substantial sequence conservation. Conserved alignment blocks within these IGSs encompassed 88 Shine-Dalgarno sequences, 55 transcriptional terminators, 5 Sigma-32 binding sites, and 12 novel small RNAs. Although pseudogene formation, and thus IGS formation, are ongoing processes in these genomes, a large proportion of intergenic spacers contain functional sequences.

The Relationship between Users Cognitive Style and Information Seeking Behavior among Postgraduate Engineering Students

The identification of common variants that contribute to the genesis of human inherited disorders remains a significant challenge. Hirschsprung disease (HSCR) is a multifactorial, non-mendelian disorder in which rare high-penetrance coding sequence mutations in the receptor tyrosine kinase RET contribute to risk in combination with mutations at other genes. We have used family-based association studies to identify a disease interval, and integrated this with comparative and functional genomic analysis to prioritize conserved and functional elements within which mutations can be sought. We now show that a common non-coding RET variant within a conserved enhancer-like sequence in intron 1 is significantly associated with HSCR susceptibility and makes a 20-fold greater contribution to risk than rare alleles do. This mutation reduces in vitro enhancer activity markedly, has low penetrance, has different genetic effects in males and females, and explains several features of the complex inheritance pattern of HSCR. Thus, common low-penetrance variants, identified by association studies, can underlie both common and rare diseases.

Biogas production from renewable resources is attracting increased attention as an alternative energy source due to the limited availability of traditional fossil fuels. Many countries are promoting the use of alternative energy sources for sustainable energy production. In this study, a metagenome from a production-scale biogas fermenter was analysed employing Roche's GS FLX Titanium technology and compared to a previous dataset obtained from the same community DNA sample that was sequenced on the GS FLX platform. Taxonomic profiling based on 16S rRNA-specific sequences and an Environmental Gene Tag (EGT) analysis employing CARMA demonstrated that both approaches benefit from the longer read lengths obtained on the Titanium platform. Results confirmed Clostridia as the most prevalent taxonomic class, whereas species of the order Methanomicrobiales are dominant among methanogenic Archaea. However, the analyses also identified additional taxa that were missed by the previous study, including members of the genera Streptococcus, Acetivibrio, Garciella, Tissierella, and Gelria, which might also play a role in the fermentation process leading to the formation of methane. Taking advantage of the CARMA feature to correlate taxonomic information of sequences with their assigned functions, it appeared that Firmicutes, followed by Bacteroidetes and Proteobacteria, dominate within the functional context of polysaccharide degradation whereas Methanomicrobiales represent the most abundant taxonomic group responsible for methane production. Clostridia is the most important class involved in the reductive CoA pathway (Wood-Ljungdahl pathway) that is characteristic for acetogenesis. Based on binning of 16S rRNA-specific sequences allocated to the dominant genus Methanoculleus, it could be shown that this genus is represented by several different species. Phylogenetic analysis of these sequences placed them in close proximity to the hydrogenotrophic methanogen Methanoculleus bourgensis. While rarefaction analyses still indicate incomplete coverage, examination of the GS FLX Titanium dataset resulted in the identification of additional genera and functional elements, providing a far more complete coverage of the community involved in anaerobic fermentative pathways leading to methane formation.

With the completion of Human Genome Project (HGP), understanding the complex interaction between trans- and cis-regulatory elements comprehensively and identifying these potential functional elements are fundamental problems in functional genomics. Although many computational approaches have been developed for lower eukaryotes and prokaryotes, most of them often do not generalize to vertebrates. Here, we use a decay function to characterize transcriptional behavior, and analyze correlations on gene expression profiles of human and mouse to construct coregulated gene groups. Using these two closely related species, we perform comparative genome analysis and identify target genes and conserved functional cis-regulatory elements by motif overrepresentation. Moreover, we presented experimental evidences (ChIP-Chip) for E2F to assert our findings.

PurposeThe purpose of this paper is to report on the experiences of 65 five library students who visited one of three selected libraries on the campus of the University of Ghana to find out the extent to which the principles of reference services were being adhered to.Design/methodology/approachThe unobstrusive method for evaluating reference services was used. Students were specifically asked to pose two questions (specific‐search or research questions) of their choice to two different library staff at the reference desk of the selected library (The library staff were unaware of this evaluation and exhibited their real behaviors to the clients.). The choice of these types of questions was to allow the library staff to engage them in a reference interview. Their observations were to be summarized in a two page report.FindingsStudents were not only concerned with correct answers to their questions, but were influenced to return to the same library staff by certain factors such as approachability of the library staff, effective assessment of their information need, good communication skills and the attentiveness of the library staff.Practical implicationsThis exercise gave students a first‐hand experience of being information seekers and directly experiencing what ordinary users are subjected to whenever they visited libraries. This experience it is hoped would guide them when they are later employed in libraries to conform to behaviors that enhance the quality of reference interview and user satisfaction.Originality/valueThis is a modest contribution to the literature on reference services from a developing country's perspective as very few empirical studies are carried out in this area.

The comparative analysis of the basic characteristics of acousto-optical instruments of different levels of integration and complexity: functional elements, devices and systems. The basic characteristics and their interrelations are determined. It is shown that each class requires individual approach to design. We indicate a novel trend of development of special class of instruments – modules, which are easy-to-integrate into existant devices or systems extending their functionality. The principle requirements for this kind of instruments are their connectivity with the device in each channel, port, protocol, so that the module adding and removing need not the device retuning or adjustment. This tendency has universal nature. The presented analysis is convinient for prognosing prospects and basic directions of acousto-optical instruments development.

Accelerated Evolution in Distinctive Species Reveals Candidate Elements for Clinically Relevant Traits, Including Mutation and Cancer Resistance