К оценке потенциального риска дозозависимых эффектов гепатотоксичности наночастиц оксида селена

The bio-reduction of selenite (Se (IV)) generates nanoparticles with sizes ranging between 30 and 300 nm. Biological properties of Se nanoparticles are dependent on the nanoparticle size; smaller particles have greater activity. The green SeNPs (selenium nanoparticles) are prepared by mixing of plant leaves methanolic extracts of Centella asiatica, Azadirachta indica, Ocimum tenuiflorum, Murraya koenigii and Catharanthus roseus with sodium selenite. This mixture was stirred which gave a dispersion of SeNPs conjugated with plants phytoconstituents. Among these plants, C. roseus showed high yield of SeNPs and purification of the nanoparticles was performed by alkaline lysis. The visualization and characterization of nanoparticles were performed by UV-Visible spectroscopy, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). The antioxidant activity of nanoparticles was determined by ABTS, DPPH and FRAP assays. The anti-bacterial activity was carried out for Streptococcus mutans, Staphylococcus aureus and Escherichia coli and anti-fungal activity was carried out for Candida albicans, Aspergillus niger and Aspergillus flavus using well diffusion method. The suspension solution confirms the formation of SeNPs showed (285 nm) by UV analysis. Screening analysis showed enormous phytoconstituents in leaves methanolic extract and simultaneously, the synthesized SeNPs by FT-IR spectrum confirmed the presence of functional groups which were associated with bioactive molecules. XRD study exhibits the nano-crystalline nature of SeNPs. SEM analysis revealed that the biosynthesized selenium nanoparticles were oval with size range of 195 nm. Green synthesized SeNPs were found to possess significant antioxidant activity (IC50 403.571, 477.48 and 60.090 µg/mL) and SeNPs showed strong antimicrobial action, with A. flavus showing the lowest zone of inhibition (9 mm) and S. mutans showing the highest zone of inhibition (35 mm). The synthesis of thermodynamically stable aqueous SeNPs has been aided by the active phytoconstituents found in C. roseus leaves extract, which are effective reducing agents. The present results support the advantages of green method to produce Se NPs having potential activities.

The pesticide chlorpyrifos (CPF) poses significant environmental and health risks due to its toxicity. Selenium nanoparticles (Se NPs) exhibit promising therapeutic properties. This study evaluated the effects of Se NPs against CPF hepatotoxicity, focusing on oxidative and inflammatory responses, and the SIRT1/FXR/Nrf2 pathway. Rats were exposed to CPF (5.4 mg/kg body weight), with or without Se NPs (0.5 mg/kg body weight), for 28 days, followed by biochemical, histopathological, and molecular analyses. CPF administration significantly increased serum ALT and AST, reduced albumin, and induced histopathological alterations. Se NPs effectively ameliorated liver function biomarkers and mitigated histopathological changes. CPF also elevated malondialdehyde and nitric oxide, and depleted enzymatic antioxidants and GSH, which were mitigated by Se NPs. CPF upregulated NF-κB p65, TNF-α, IL-6, iNOS, Bax and caspase-3, and downregulated Bcl-2. Se NPs suppressed inflammation and apoptosis by downregulating NF-κB p65, pro-inflammatory cytokines and pro-apoptosis markers. These effects were linked to upregulation of SIRT1, FXR, Nrf2 and HO-1 and suppression of Keap1. In conclusion, Se NPs protect against CPF-induced liver injury by attenuating OS, inflammation, and apoptosis, and by upregulating SIRT1//FXR/Nrf2 signaling. These findings highlight the therapeutic potential of Se NPs in mitigating hepatotoxicity induced by exposure to CPF.

A novel, very simple, one-way, economical, and convenient reaction method for the simultaneous synthesis of dialkyl phosphoric acids and selenium nanoparticles has been developed. This communication describes the synthesis and characterization of selenium nanoparticles through the reactions of dialkyl phosphites and selenium dioxide. Dialkyl phosphites serve as reducing agents for converting selenium dioxide (a hazardous selenium source) to selenium nanoparticles (Se NPs), and conversely, selenium dioxide oxidizes dialkyl phosphites to dialkyl phosphoric acids. The impact of solvents, temperature, and ratio of materials is included in this study. Most of the prepared Se NPs tend to agglomerate, and their stability improved by the addition of β-cyclodextrin. In addition to enhancing the stability of Se NPs by cyclodextrin, the anticancer properties of the as-prepared Se NPs stabilized by β-cyclodextrin are studied. The reported method presents the conversion of selenium dioxide (a hazardous selenium source) to valuable Se NPs (hemispherical Se crystals) and industrially applicable dialkyl phosphoric acids from inexpensive materials with zero E factor and one atom economy.

Antioxidant and cytotoxic effect of biologically synthesized selenium nanoparticles in comparison to selenium dioxide

In vitro study on the individual and synergistic cytotoxicity of adriamycin and selenium nanoparticles against Bel7402 cells with a quartz crystal microbalance

Mitigating effect of selenium nanoparticles modified with Lycium barbarum polysaccharide on acute liver injury.

Current investigation was focused to determine the biological effects of Selenium nanoparticles (Se NPs) in mice. Se NPs (50mg/ml saline/Kg body weight) were intraperitoneally injected to 5 week old albino mice (N = 22) for 14 days. Control group was intraperitoneally injected with saline water (N = 22). In all subjects, a series of neurological tests, hematological parameters and markers of oxidative stress in vital organs were determined. We are reporting that rota rod and open field test performance remained unaffected in Se NPs injected mice when compared with saline treated controls. Male mice injected with Se NPs had significantly less line crossing (P = 0.02) while performing light dark box. They approached object A less frequently (P = 0.02) and spent lesser time with it (P = 0.001) during novel object recognition test (trial 1). % lymphocytes were significantly reduced (P = 0.03) in these mice while % monocytes were higher than control (P = 0.03). Concentration of cholesterol (P = 0.02) and LDL (P = 0.003) was significantly decreased in male mice. Female Se NPs treated mice spent less time (P = 0.05) with B object in trial 1 and 2 (P = 0.04) of novel object recognition test. They had significantly reduced cholesterol level (P = 0.02) and significantly increased catalase activity in the liver (P = 0.01) than control. Remaining parameters of behavior, blood chemistry and markers of oxidative stress from vital organs were non-significantly different upon their comparison between Se NPs and saline injected mice.

Selenium as an essential trace element for the health of the humans was used to hydrothermally synthesis of selenium nanoparticle (Se NPs) using Aloe vera leaf extract (ALE). Effects of synthesis parameters namely; amount of ALE (1–5 ml) and amount of Na 2 SeO 3 solution (10–30 ml), on the particle size and colour intensity of the solution containing Se NPs were studied using response surface methodology. FT-IR spectroscopy, UV-Vis spectrophotometry, DLS analyzer and TEM were used to determine the specifications of the ALE and synthesized Se NPs. Obtained results indicated that the ALE contained several bioactive compounds, which they had hydroxyl and amide І groups in their structures and these two functional groups had key roles in the reduction of the selenium ions to form Se NPs and stabilizing of them. Furthermore, spherical fabricated Se NPs using obtained optimum synthesis parameters, 4.92 mL of ALE and 13.03 mL of Na 2 SeO 3 solution, had colour intensity, mean particle size, zeta potential and polydispersity index values of 3.0% a.u., 50 nm, -18 mV and 0.344, respectively according to the DLS analysis. The synthesized Se NPs had also high antibacterial and antifungal activities against 4 selected pathogenic bacteria and spoilage fungi strains.

Facile synthesis of Co3O4@SeNPs grafted MWCNTs Nanocomposite for high energy density supercapacitor and antimicrobial applications

Exploring the impact of mycogenic selenium nanoparticles on production of exopolysaccharides from arid zone bacteria

Fusarium species are commonly resistant to many antifungal drugs. The limited therapeutic options available have led to a surge of research efforts aimed at discovering novel antifungal compounds in recent decades. This study aimed to assess the in vitro antifungal activity of plant-based biosynthesized selenium nanoparticles (Se NPs) and six comparators against a set of clinical Fusarium strains. In vitro antifungal activity of Se NPs synthesized using plant extracts of Allium paradoxum, Crocus caspius, Pistacia vera L. hull, Vicia faba L. hull and Heracleum persicum, as well as six common antifungal drugs, namely voriconazole, itraconazole, amphotericin B, posaconazole, natamycin, and caspofungin were evaluated against 94 clinical Fusarium strains using broth microdilution according to Clinical and Laboratory Standards Institute guideline. The obtained results were intriguing since all five types of biosynthesized Se NPs demonstrated significantly higher antifungal activity, compared to antifungal drugs. It was found that Se NPs synthesized by V. faba L. hull extract (0.03 μg/ml) had the lowest geometric mean minimum inhibitory concentration value followed by Se NPs synthesized by P. veraL. hull extract (0.25 μg/ml), A. paradoxum extract (0.39 μg/ml), C. caspius extract (0.55 μg/ml), and H. persicum extract (0.9 μg/ml). Plant-based Se NPs demonstrated supreme antifungal activity and could be considered promising antifungal agents for Fusarium infections. However, tests, such as toxicity and in vivo tests are needed before the product can be used in clinical settings.

Influence of dispersion agents on particle size and concentration determined by laser-induced breakdown detection

A new insight into the mechanism of cadmium-induced nephrotoxicity and antagonism of selenium nanoparticles: modulation of MTF1-MT axis dependent metal homeostasis.

Selenium nanoparticles attenuates silver nanoparticles toxicity in mice Sertoli cells via modulation of SOD and Kindlin-2.

Selenium nanoparticles as catalysts for nitric oxide generation.